Quantitative Analysis of Fructo-oligosaccharides in Gynura divaricata subsp. formosana by High Performance Anion Exchange Chromatography-Pulsed Amperometric Detection
2012 ◽
Vol 7
(8)
◽
pp. 1934578X1200700
Keyword(s):
High performance anion exchange chromatography-pulsed amperometric detection was employed in this study to conduct quantitative analysis of the inulin-related fructo-oligosaccharides present in Gynura divaricata subsp. formosana. Result obtained for the 1-kestose (GF2), nystose (GF3) and 1F-β-fructofuranosyl-nystose (GF4) contents were 146.60 ± 0.04, 24.70 ± 0.75 and 16.60 ± 0.91 μg/g, fresh weight, and 68.90 ± 0.02, 7.60 ± 1.34 and 149.30 ± 0.06 μg/g (mean ± RSD), dry weight, respectively. Using this method, the limit of quantitation was 20 μg/mL and the linear detectability between 0-250 μg/mL. The developed method provides a practical analysis for these low caloric value, prebiotic and non-digestible carbohydrates in the genus Gynura.
1998 ◽
Vol 800
(2)
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pp. 199-206
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1999 ◽
Vol 852
(2)
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pp. 407-416
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2014 ◽
Vol 32
(12)
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pp. 1380
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2011 ◽
Vol 29
(1)
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pp. 75-78
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1995 ◽
Vol 25
(12)
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pp. 2022-2028
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