Role of Escherichia coli RecA protein in SOS induction and post-replication repair

Biochimie ◽  
1985 ◽  
Vol 67 (3-4) ◽  
pp. 353-356 ◽  
Author(s):  
Patrice L. Moreau
Keyword(s):  
Escherichia Coli ◽  
Reca Protein ◽  
Sos Induction ◽  
Post Replication Repair

scholarly journals Excision and transposition of Tn5 as an SOS activity in Escherichia coli.

Genetics ◽  
1991 ◽  
Vol 128 (1) ◽  
pp. 45-57 ◽  
Author(s):  
C T Kuan ◽  
S K Liu ◽  
I Tessman
Keyword(s):  
Escherichia Coli ◽  
Binding Site ◽  
Reca Protein ◽  
Precursor Molecule ◽  
Functional Integrity ◽  
Lexa Protein ◽  
Lexa Binding ◽  
Additional Role ◽  
Stimulation Of

Abstract Excision and transposition of the Tn5 element in Escherichia coli ordinarily appear to occur by recA-independent mechanisms. However, recA(Prtc) genes, which encode RecA proteins that are constitutively activated to the protease state, greatly enhanced excision and transposition; both events appeared to occur concomitantly and without destruction of the donor DNA. The recombinase function of the RecA protein was not required. Transposition was accompanied by partial, and occasionally full, restoration of the functional integrity of the gene vacated by the excised Tn5. The stimulation of transposition was inhibited by an uncleavable LexA protein and was strongly enhanced by an additional role of the RecA(Prtc) protein besides its mediation of LexA cleavage. To account for the enhanced transposition, we suggest that (i) there may be a LexA binding site within the promoter for the IS50 transposase, (ii) activated RecA may cleave the IS50 transposition inhibitor, and (iii) the transposase may be formed by RecA cleavage of a precursor molecule.

Role of Escherichia coli RecBC enzyme in SOS induction

1985 ◽  
Vol 201 (3) ◽  
pp. 525-528 ◽  
Author(s):  
Abdul M. Chaudhury ◽  
Gerald R. Smith
Keyword(s):  
Escherichia Coli ◽  
Sos Induction

Exposure to sub-inhibitory ciprofloxacin and nitrofurantoin concentrations increases recA gene expression in uropathogenic Escherichia coli: The role of RecA protein as a drug target

2020 ◽  
Vol 146 ◽  
pp. 105268 ◽  
Author(s):  
Ághata Cardoso da Silva Ribeiro ◽  
Willames Marcos Brasileiro da Silva Martins ◽  
Adilson Aderito da Silva ◽  
Ana Cristina Gales ◽  
Daniela Gonçales Galasse Rando ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Drug Target ◽  
Reca Protein ◽  
Uropathogenic Escherichia Coli ◽  
Reca Gene

scholarly journals In vivo role of Escherichia coli single-strand exonucleases in SOS induction by gamma radiation

Mutagenesis ◽  
2008 ◽  
Vol 23 (4) ◽  
pp. 317-323 ◽  
Author(s):  
J. Serment-Guerrero ◽  
M. Brena-Valle ◽  
J. J. Espinosa-Aguirre
Keyword(s):  
Escherichia Coli ◽  
Gamma Radiation ◽  
Single Strand ◽  
Sos Induction

scholarly journals Genetic Evidence for the Requirement of RecA Loading Activity in SOS Induction after UV Irradiation in Escherichia coli

2006 ◽  
Vol 188 (14) ◽  
pp. 5024-5032 ◽  
Author(s):  
Ivana Ivančić-Baće ◽  
Ignacija Vlašić ◽  
Erika Salaj-Šmic ◽  
Krunoslav Brčić-Kostić
Keyword(s):  
Escherichia Coli ◽  
Uv Irradiation ◽  
Constitutive Expression ◽  
Recq Helicase ◽  
Sos Induction ◽  
Dna Damaging Agents ◽  
Lexa Repressor ◽  
Recbcd Enzyme ◽  
Dna Substrate

ABSTRACT The SOS response in Escherichia coli results in the coordinately induced expression of more than 40 genes which occurs when cells are treated with DNA-damaging agents. This response is dependent on RecA (coprotease), LexA (repressor), and the presence of single-stranded DNA (ssDNA). A prerequisite for SOS induction is the formation of a RecA-ssDNA filament. Depending on the DNA substrate, the RecA-ssDNA filament is produced by either RecBCD, RecFOR, or a hybrid recombination mechansim with specific enzyme activities, including helicase, exonuclease, and RecA loading. In this study we examined the role of RecA loading activity in SOS induction after UV irradiation. We performed a genetic analysis of SOS induction in strains with a mutation which eliminates RecA loading activity in the RecBCD enzyme (recB1080 allele). We found that RecA loading activity is essential for SOS induction. In the recB1080 mutant RecQ helicase is not important, whereas RecJ nuclease slightly decreases SOS induction after UV irradiation. In addition, we found that the recB1080 mutant exhibited constitutive expression of the SOS regulon. Surprisingly, this constitutive SOS expression was dependent on the RecJ protein but not on RecFOR, implying that there is a different mechanism of RecA loading for constitutive SOS expression.

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