Expression of capsaicin receptor (VR1) by myelinated primary afferent neurons in rats

2002 ◽  
Vol 319 (2) ◽  
pp. 87-90 ◽  
Author(s):  
Qing-Ping Ma
Keyword(s):  
Afferent Neurons ◽  
Primary Afferent Neurons ◽  
Primary Afferent ◽  
Capsaicin Receptor

PAINFUL NEUROPATHY DECREASES MEMBRANE CALCIUM CURRENT IN MAMMALIAN PRIMARY AFFERENT NEURONS

2000 ◽  
Vol 5 (4) ◽  
pp. 245-245
Author(s):  
Qh Hogan ◽  
Jb McCallum ◽  
C Sarantopoulos ◽  
M Aason ◽  
M Mynlieff ◽  
...  
Keyword(s):  
Calcium Current ◽  
Afferent Neurons ◽  
Painful Neuropathy ◽  
Primary Afferent Neurons ◽  
Primary Afferent

scholarly journals Frequency response properties of primary afferent neurons in the posterior lateral line system of larval zebrafish

2015 ◽  
Vol 113 (2) ◽  
pp. 657-668 ◽  
Author(s):  
Rafael Levi ◽  
Otar Akanyeti ◽  
Aleksander Ballo ◽  
James C. Liao
Keyword(s):  
Lateral Line ◽  
Sine Wave ◽  
Afferent Neuron ◽  
Lateral Line System ◽  
Afferent Neurons ◽  
Primary Afferent Neurons ◽  
Primary Afferent ◽  
Line System ◽  
Response Properties ◽  
Larval Zebrafish

The ability of fishes to detect water flow with the neuromasts of their lateral line system depends on the physiology of afferent neurons as well as the hydrodynamic environment. Using larval zebrafish ( Danio rerio), we measured the basic response properties of primary afferent neurons to mechanical deflections of individual superficial neuromasts. We used two types of stimulation protocols. First, we used sine wave stimulation to characterize the response properties of the afferent neurons. The average frequency-response curve was flat across stimulation frequencies between 0 and 100 Hz, matching the filtering properties of a displacement detector. Spike rate increased asymptotically with frequency, and phase locking was maximal between 10 and 60 Hz. Second, we used pulse train stimulation to analyze the maximum spike rate capabilities. We found that afferent neurons could generate up to 80 spikes/s and could follow a pulse train stimulation rate of up to 40 pulses/s in a reliable and precise manner. Both sine wave and pulse stimulation protocols indicate that an afferent neuron can maintain their evoked activity for longer durations at low stimulation frequencies than at high frequencies. We found one type of afferent neuron based on spontaneous activity patterns and discovered a correlation between the level of spontaneous and evoked activity. Overall, our results establish the baseline response properties of lateral line primary afferent neurons in larval zebrafish, which is a crucial step in understanding how vertebrate mechanoreceptive systems sense and subsequently process information from the environment.

scholarly journals Transient receptor potential ankyrin-1 has a major role in mediating visceral pain in mice

2010 ◽  
Vol 298 (1) ◽  
pp. G81-G91 ◽  
Author(s):  
Fiore Cattaruzza ◽  
Ian Spreadbury ◽  
Marcela Miranda-Morales ◽  
Eileen F. Grady ◽  
Stephen Vanner ◽  
...  
Keyword(s):  
Inflammatory Pain ◽  
Transient Receptor Potential ◽  
Visceral Pain ◽  
Receptor Potential ◽  
Afferent Neurons ◽  
Visceral Hyperalgesia ◽  
Spinal Neurons ◽  
Primary Afferent Neurons ◽  
Primary Afferent ◽  
Transient Receptor

The excitatory ion channel transient receptor potential ankyrin-1 (TRPA1) is prominently expressed by primary afferent neurons and is a mediator of inflammatory pain. Inflammatory agents can directly activate [e.g., hydroxynonenal (HNE), prostaglandin metabolites] or indirectly sensitize [e.g., agonists of protease-activated receptor (PAR2)] TRPA1 to induce somatic pain and hyperalgesia. However, the contribution of TRPA1 to visceral pain is unknown. We investigated the role of TRPA1 in visceral hyperalgesia by measuring abdominal visceromotor responses (VMR) to colorectal distention (CRD) after intracolonic administration of TRPA1 agonists [mustard oil (MO), HNE], sensitizing agents [PAR2 activating peptide (PAR2-AP)], and the inflammatory agent trinitrobenzene sulfonic acid (TNBS) in trpa1+/+ and trpa1−/− mice. Sensory neurons innervating the colon, identified by retrograde tracing, coexpressed immunoreactive TRPA1, calcitonin gene-related peptide, and substance P, expressed TRPA1 mRNA and responded to MO with depolarizing currents. Intracolonic MO and HNE increased VMR to CRD and induced immunoreactive c-fos in spinal neurons in trpa1 +/+ but not in trpa1 −/− mice. Intracolonic PAR2-AP induced mechanical hyperalgesia in trpa1 +/+ but not in trpa1 −/− mice. TNBS-induced colitis increased in VMR to CRD and induced c-fos in spinal neurons in trpa1 +/+ but not in trpa1 −/− mice. Thus TRPA1 is expressed by colonic primary afferent neurons. Direct activation of TRPA1 causes visceral hyperalgesia, and TRPA1 mediates PAR2-induced hyperalgesia. TRPA1 deletion markedly reduces colitis-induced mechanical hyperalgesia in the colon. Our results suggest that TRPA1 has a major role in visceral nociception and may be a therapeutic target for colonic inflammatory pain.

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