The effect of antibiotics on the in vitro growth response of chrysanthemum and tobacco stem transverse thin cell layers (tTCLs)

2003 ◽  
Vol 97 (3-4) ◽  
pp. 397-410 ◽  
Author(s):  
Jaime A Teixeira da Silva ◽  
Duong T Nhut ◽  
Michio Tanaka ◽  
Seiichi Fukai
Keyword(s):  
Growth Response ◽  
In Vitro Growth ◽  
Thin Cell Layers ◽  
Tobacco Stem ◽  
Cell Layers ◽  
Transverse Thin Cell Layers

scholarly journals Filter Paper Type Affects the Morphogenic Programs and Buffers the Phytotoxic Effect of Antibiotics in Chrysanthemum and Tobacco Thin Cell Layers

HortScience ◽  
2003 ◽  
Vol 38 (7) ◽  
pp. 1403-1407 ◽  
Author(s):  
Jaime A.Teixeira da Silva
Keyword(s):  
Filter Paper ◽  
Callus Formation ◽  
Thin Cell Layers ◽  
In Vitro Plantlets ◽  
Tobacco Stem ◽  
Phytotoxic Effect ◽  
Cell Layers ◽  
Development And Differentiation ◽  
Filter Papers

Filter paper types significantly affected the growth, development and differentiation of chrysanthemum and tobacco stem thin cell layers (TCLs) from in vitro plantlets. Three different filter paper types, normally with varied uses in plant biology, showed varying morphogenic-altering and antibiotic-buffering capacities. Advantec #2 and Whatman #1 significantly stimulated root, shoot and callus formation while Whatman #3 inhibited them, as compared to TCLs placed directly on agar. Filter paper buffered the phytotoxic effect of antibiotics kanamycin and cefotaxime, substances commonly used in genetic transformation experiments, up to as much as 50%, independent of species or genotype. In both `Lineker' and `Shuhou-no-chikara' chrysanthemum cultivars, Advantec #2 and Whatman #1 filter papers stimulated embryogenesis but in tobacco all three filter paper types significantly reduced embryogenesis and explant survival.

Morphological and physiological characteristics of rapeseed plants regenerated in vitro from thin cell layers in the presence of zinc

2007 ◽  
Vol 330 (10) ◽  
pp. 728-734 ◽  
Author(s):  
Asma Ben Ghnaya ◽  
Gilbert Charles ◽  
Annick Hourmant ◽  
Jeannette Ben Hamida ◽  
Michel Branchard
Keyword(s):  
Physiological Characteristics ◽  
Thin Cell Layers ◽  
Cell Layers
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