Morphological and physiological characteristics of rapeseed plants regenerated in vitro from thin cell layers in the presence of zinc

Conociendo las propiedades medicinales de la especie vegetal Psychotria ipecacuanha (Brot.) Stokes, su crítico estado de conservación, así como las dificultades que presenta para la propagación efectiva, el presente estudio tuvo como objetivo evaluar su potencial de propagación por los sistemas de regeneración in vitro, organogénesis y embriogénesis somática. Para este propósito, capas delgadas de células (CDCs) de tallos y de hojas, así como segmentos foliares fueron sometidos a diferentes tratamientos con reguladores de crecimiento y condiciones de luz. Además se estableció el efecto de diferentes longitudes de onda vía diodos emisores de luz (LEDs), sobre la regeneración en estos explantes y nudos provenientes de plantas in vitro. Los resultados obtenidos mostraron que los segmentos de hoja y las CDCs de tallo sembrados en el medio de cultivo MS suplementado con las combinaciones de los reguladores de crecimiento IBA + BAP e IBA + TDZ formaron embriones somáticos y brotes. Los cortes histológicos realizados corroboraron estos dos tipos de origen. Se encontró que bajo la condición lumínica 16/8, se alcanzaron los mejores resultados de inducción de brotes y embriones. En cuanto al efecto de las diferentes longitudes de onda de luz, se encontró que las correspondientes al rojo, verde y blanca, favorecieron el crecimiento y desarrollo de brotes y la inducción de embriones somáticos. El desarrollo de los brotes a partir de los nudos no presentó diferencias estadísticas entre los tratamientos con LEDs, por lo que se recomienda el uso de la luz blanca continua y con fotoperiodo durante el proceso de multiplicación y desarrollo de estos.Regeneration Potential of Psychotria Ipecacuanha (Rubiaceae) from Thin Cell Layers Knowing the medicinal properties of the plant specie Psychotria ipecacuanha (Brot.) Stokes, its critical condition and the difficulties for its effective propagation, the present study aimed to assess the potential of propagation of P. ipecacuanha by in vitro systems of regeneration, organogenesis and somatic embryogenesis. For this purpose, thin cell layers (TCL) of stems and leaves, as well as leaf segments were subjected to different treatments of plant growth regulators and light conditions. Furthermore, the effect of different wavelengths via light emitting diodes (LED’s), was established for the regeneration in these explants and nodal explants from in vitro plants. The results showed that leaf segments and stem TCL, cultured in MS medium supplemented with the combinations of growth regulators IBA + BAP and IBA + TDZ, formed somatic embryos and shoots. The histological sections supported the two types of source. It was found that the best results in shoot induction and embryos were achieved under the light condition 16/8-h light/dark. Regarding the effect of different wavelengths, it was found that those corresponding to red, green, and white supported the growth and shoot development as well as somatic embryos induction. The shoots development from the nodal explants did not show statistical differences between LEDs treatments, so the use of a continuous white light and photoperiod is recommended during their multiplication and development.

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Rapid Shoot Regeneration from Thin Cell Layer Explants of an Endangered Medicinal Asclepiad Ceropegia spiralis L.

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v21i1.9564 ◽

2012 ◽

Vol 21 (1) ◽

pp. 63-73 ◽

Cited By ~ 6

Author(s):

K. Sri Rama Murthy ◽

R. Kondamudi

Keyword(s):

Survival Rate ◽

Key Words ◽

Plant Tissue ◽

Cell Layer ◽

Thin Cell Layer ◽

Thin Cell Layers ◽

In Vitro Plantlets ◽

Cell Layers ◽

Extensive Growth

The thin cell layers of nodes and internodes of Ceropegia spiralis L. were cultured on MS supplemented with BAP 13.32 µM/l + NAA 0.537 μM/l induced 17.34 ± 0.55 shoots showing extensive growth. Later on the organogenesis was also induced on MS containing BAP 13.32 µM/l + 2, 4-D 1.130 µM/l, whereas the medium with BAP 13.32 µM/l + 2, 4-D 4.52 µM/l has the highest callus producing ability in recalcitrants as well as in normal explants. Shoots developed were rooted best on 0.5 MS with NAA 10.74 μM/l. Optimum shoot and root multiplication was obtained within eight weeks. In vitro plantlets were successfully weaned and transferred to soil with about 90 per cent survival rate. So far, more than 650 weanlings have been produced successfully and reintroduced into nature for their recovery. Key words: Ceropegia spiralis, Thin cell layer explants, Recalcitrants, Regeneration D. O. I. 10.3329/ptcb.v21i1.9564 Plant Tissue Cult. & Biotech. 21(1): 63-73, 2011 (June)

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Filter Paper Type Affects the Morphogenic Programs and Buffers the Phytotoxic Effect of Antibiotics in Chrysanthemum and Tobacco Thin Cell Layers

HortScience ◽

10.21273/hortsci.38.7.1403 ◽

2003 ◽

Vol 38 (7) ◽

pp. 1403-1407 ◽

Cited By ~ 5

Author(s):

Jaime A.Teixeira da Silva

Keyword(s):

Filter Paper ◽

Callus Formation ◽

Thin Cell Layers ◽

In Vitro Plantlets ◽

Tobacco Stem ◽

Phytotoxic Effect ◽

Cell Layers ◽

Development And Differentiation ◽

Filter Papers

Filter paper types significantly affected the growth, development and differentiation of chrysanthemum and tobacco stem thin cell layers (TCLs) from in vitro plantlets. Three different filter paper types, normally with varied uses in plant biology, showed varying morphogenic-altering and antibiotic-buffering capacities. Advantec #2 and Whatman #1 significantly stimulated root, shoot and callus formation while Whatman #3 inhibited them, as compared to TCLs placed directly on agar. Filter paper buffered the phytotoxic effect of antibiotics kanamycin and cefotaxime, substances commonly used in genetic transformation experiments, up to as much as 50%, independent of species or genotype. In both `Lineker' and `Shuhou-no-chikara' chrysanthemum cultivars, Advantec #2 and Whatman #1 filter papers stimulated embryogenesis but in tobacco all three filter paper types significantly reduced embryogenesis and explant survival.

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Flower bud and root neoformation in thin cell layers of Nicotiana tabacum var. Samsun: a statistical analysis of spatial and temporal changes in mitotic activity

Canadian Journal of Botany ◽

10.1139/b90-314 ◽

1990 ◽

Vol 68 (11) ◽

pp. 2501-2508 ◽

Cited By ~ 2

Author(s):

G. Mersch ◽

C. Nassogne ◽

A. Havelange

Keyword(s):

Statistical Analysis ◽

Nicotiana Tabacum ◽

Mitotic Activity ◽

Temporal Changes ◽

Flower Bud ◽

Time Intervals ◽

Thin Cell Layers ◽

Spatial And Temporal Changes ◽

Cell Layers

Our aim was to analyse comparatively the spatial and temporal changes in mitotic activity in tobacco thin cell layers cultured in vitro during the expression of two organogenetic programs, namely the flower bud and root programs. These biological materials were found to be so complex and heterogeneous that we had to rely on sophisticated statistical models for the planned study. Using these models we could detect differences not only between the two programs, but also within each program between different time intervals and, in the flower bud program, between different longitudinal segments of the thin cell layers. Key words: Nicotiana tabacum, flower bud, root, statistical analysis, mitotic activity.

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