Genomic organization and characterization of promoter function of the human CDK9 gene

Gene ◽  
2000 ◽  
Vol 252 (1-2) ◽  
pp. 51-59 ◽  
Author(s):  
Hongbing Liu ◽  
Andrew P. Rice
Keyword(s):  
Genomic Organization ◽  
Promoter Function

Genomic organization of the 5′ end of human β-ENaC and preliminary characterization of its promoter

2002 ◽  
Vol 282 (5) ◽  
pp. F898-F909 ◽  
Author(s):  
Christie P. Thomas ◽  
Randy W. Loftus ◽  
Kang Z. Liu ◽  
Omar A. Itani
Keyword(s):  
Genomic Organization ◽  
Human Kidney ◽  
Β Subunit ◽  
Transcription Start ◽  
Transcription Start Sites ◽  
Nuclear Extracts ◽  
Flanking Region ◽  
Rapid Amplification ◽  
Flanking Regions

The mRNA for the β-subunit of the epithelial Na+ channel (β-ENaC) is regulated developmentally and, in some tissues, in response to corticosteroids. To understand the mechanisms of transcriptional regulation of the human β-ENaC gene, we characterized the 5′ end of the gene and its 5′-flanking regions. Adaptor-ligated human kidney and lung cDNA were amplified by 5′ rapid amplification of cDNA ends, and transcription start sites of two 5′ variant transcripts were determined by nuclease protection or primer extension assays. Cosmid clones that contain the 5′ end of the gene were isolated, and analysis of these clones indicated that alternate first exons ∼1.5 kb apart and ∼ 45 kb upstream of a common second exon formed the basis of these transcripts. Genomic fragments that included the proximal 5′-flanking region of either transcript were able to direct expression of a reporter gene in lung epithelia and to bind Sp1 in nuclear extracts, confirming the presence of separate promoters that regulate β-ENaC expression.

Genomic organization and promoter characterization of FcCTL, a C-type lectin-like protein in Fenneropenaeus chinensis

Gene ◽  
2013 ◽  
Vol 518 (2) ◽  
pp. 376-380 ◽  
Author(s):  
XiaoFang Lai ◽  
Huan Gao ◽  
Jie Kong ◽  
QingYin Wang ◽  
XianHong Meng ◽  
...  
Keyword(s):  
Genomic Organization ◽  
Fenneropenaeus Chinensis ◽  
Promoter Characterization

scholarly journals Molecular Characterization of Two Lactate Dehydrogenase Genes with a Novel Structural Organization on the Genome of Lactobacillus sp. Strain MONT4

2004 ◽  
Vol 70 (10) ◽  
pp. 6290-6295 ◽  
Author(s):  
Jennifer Weekes ◽  
Gülhan Ü. Yüksel
Keyword(s):  
Escherichia Coli ◽  
Lactate Dehydrogenase ◽  
Sequence Analysis ◽  
Molecular Characterization ◽  
Northern Blot ◽  
Structural Organization ◽  
Genomic Organization ◽  
The Individual

ABSTRACT Two lactate dehydrogenase (ldh) genes from Lactobacillus sp. strain MONT4 were cloned by complementation in Escherichia coli DC1368 (ldh pfl) and were sequenced. The sequence analysis revealed a novel genomic organization of the ldh genes. Subcloning of the individual ldh genes and their Northern blot analyses indicated that the genes are monocistronic.

scholarly journals Cloning, Genomic Organization, and Characterization of a Human Cholinephosphotransferase

2000 ◽  
Vol 275 (38) ◽  
pp. 29808-29815 ◽  
Author(s):  
Annette L. Henneberry ◽  
Graeme Wistow ◽  
Christopher R. McMaster
Keyword(s):  
Genomic Organization

Fkbp8: novel isoforms, genomic organization, and characterization of a forebrain promoter in transgenic mice

Genomics ◽  
2004 ◽  
Vol 83 (1) ◽  
pp. 181-192 ◽  
Author(s):  
Jakob V Nielsen ◽  
Cathy Mitchelmore ◽  
Karen M Pedersen ◽  
Karen M Kjærulff ◽  
Bente Finsen ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Genomic Organization ◽  
Novel Isoforms

scholarly journals Genomic organization and molecular characterization of porcine cytomegalovirus

Virology ◽  
2014 ◽  
Vol 460-461 ◽  
pp. 165-172 ◽  
Author(s):  
Weiwei Gu ◽  
Nanfang Zeng ◽  
Lei Zhou ◽  
Xinna Ge ◽  
Xin Guo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genomic Organization ◽  
Porcine Cytomegalovirus

Genomic organization and promoter characterization of two human UHS keratin genes

Gene ◽  
1999 ◽  
Vol 227 (2) ◽  
pp. 137-148 ◽  
Author(s):  
C. Perez ◽  
J. Auriol ◽  
C. Gerst ◽  
B.A. Bernard ◽  
J.-M. Egly
Keyword(s):  
Genomic Organization ◽  
Promoter Characterization
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