Expression and antigenic characterization of recombinant Mycoplasma agalactiae P48 major surface protein

ABSTRACT Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. Although serologic tests have identified American bison, Bison bison, as being infected with A. marginale, the present study was undertaken to confirm A. marginale infection and to characterize isolates obtained from naturally infected bison in the United States and Canada. Major surface protein (MSP1a and MSP4) sequences of bison isolates were characterized in comparison with New World cattle isolates. Blood from one U.S. bison was inoculated into a susceptible, splenectomized calf, which developed acute anaplasmosis, demonstrating infectivity of this A. marginale bison isolate for cattle. The results of this study showed that these A. marginale isolates obtained from bison were similar to ones from naturally infected cattle.

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Mapping of B-cell epitopes in the N-terminal repeated peptides of Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigens

Veterinary Immunology and Immunopathology ◽

10.1016/j.vetimm.2003.11.003 ◽

2004 ◽

Vol 98 (3-4) ◽

pp. 137-151 ◽

Cited By ~ 34

Author(s):

Jose C. Garcia-Garcia ◽

José de la Fuente ◽

Katherine M. Kocan ◽

Edmour F. Blouin ◽

Thomas Halbur ◽

...

Keyword(s):

Immune Response ◽

B Cell ◽

Humoral Immune Response ◽

Surface Protein ◽

Anaplasma Marginale ◽

B Cell Epitopes ◽

Major Surface Protein ◽

Humoral Immune ◽

Major Surface

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Isolation and characterization of monoclonal antibodies specific for antigen P1, a major surface protein of mutans streptococci.

Infection and Immunity ◽

10.1128/iai.55.11.2759-2767.1987 ◽

1987 ◽

Vol 55 (11) ◽

pp. 2759-2767 ◽

Cited By ~ 50

Author(s):

G Y Ayakawa ◽

L W Boushell ◽

P J Crowley ◽

G W Erdos ◽

W P McArthur ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Surface Protein ◽

Mutans Streptococci ◽

Major Surface Protein ◽

Isolation And Characterization ◽

Major Surface

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Cloning and characterization of a major surface protein (MspTL) ofTreponema lecithinolyticumassociated with rapidly progressive periodontitis

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.2002.tb11049.x ◽

2002 ◽

Vol 207 (2) ◽

pp. 185-192 ◽

Cited By ~ 7

Author(s):

Kwang-Kyun Park ◽

Klaus Heuner ◽

Ulf B. GÃ¶bel ◽

Yun-Jung Yoo ◽

Chong-Kwan Kim ◽

...

Keyword(s):

Surface Protein ◽

Major Surface Protein ◽

Major Surface

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Sequence and Antigenic Variability of theHelicobacter mustelae Surface Ring Protein Hsr

Infection and Immunity ◽

10.1128/iai.69.5.3447-3450.2001 ◽

2001 ◽

Vol 69 (5) ◽

pp. 3447-3450 ◽

Cited By ~ 2

Author(s):

Natasha Forester ◽

John S. Lumsden ◽

Tadhg O'Croinin ◽

Paul W. O'Toole

Keyword(s):

Repetitive Sequences ◽

Surface Protein ◽

Major Surface Protein ◽

Antigenic Variability ◽

Amino Terminal ◽

Major Surface

ABSTRACT We have identified an array of more than 500 repetitive sequences flanking the hsr gene, which encodes the major surface protein of the ferret pathogen Helicobacter mustelae. The repeats show identity exclusively to the amino-terminal half of Hsr. Analysis of Hsr from three strains indicated variability of exposed epitopes. Characterization of an hsr mutant showed that Hsr is not an adhesin.

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CHARACTERIZATION OF HUMAN GRANULOCYTIC EHRLICHIOSIS IN EXPERIMENTAL INFECTED HL-60 CELLS WITH ANAPLASMA PHAGOCYTOPHILUM USING NESTED PCR AND A. PHAGOCYTOPHILUM MAJOR SURFACE PROTEIN-2 MONOCLONAL ANTIBODY

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v3i2.11400 ◽

2012 ◽

Vol 3 (2) ◽

pp. 160-165

Author(s):

MS Rahman ◽

JH Park ◽

JS Chae

Keyword(s):

Monoclonal Antibody ◽

Nested Pcr ◽

Anaplasma Phagocytophilum ◽

Surface Protein ◽

Intense Band ◽

Major Surface Protein ◽

Granulocytic Ehrlichiosis ◽

Human Granulocytic Ehrlichiosis ◽

Major Surface

The study was carried out to characterize the human granulocytic ehrlichiosis in experimental infected HL-60 cells with Anaplasma phagocytophilum using nested PCR and A. phagocytophilum major surface protein-2 monoclonal antibody. The nested PCR revealed only one band of 926 base pair DNA from A. phagocytophilum infected HL-60 cells. The western blot revealed several bands with a dominant 44-kDa. There were intense band of 100- and 160-kDa bands. The 44-kDa component was at least 10 times more abundant than the 100- and 160-kDa bands. In conclusion, the nested PCR would be a valuable tool for the characterization of human granulocytic ehrlichiosis.

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