472 Alkaline elution technique and DNA fluorometric quantification: modifications, evaluation and statistics

2003 ◽  
Vol 144 ◽  
pp. s127
Author(s):  
M. Goumenou ◽  
K. Machera
Keyword(s):  
Alkaline Elution

Measurement of DNA damage in unlabeled mammalian cells analyzed by alkaline elution and a fluorometric DNA assay

1980 ◽  
Vol 106 (1) ◽  
pp. 169-174 ◽  
Author(s):  
Leonard C. Erickson ◽  
Rainhardt Osieka ◽  
Nancy A. Sharkey ◽  
Kurt W. Kohn
Keyword(s):  
Dna Damage ◽  
Mammalian Cells ◽  
Alkaline Elution ◽  
Dna Assay

Genotoxic effects of 2-amino-3,4-dimethylimidazo(4,5-f)quinoline (MeIQ) in rats measured by alkaline elution

1991 ◽  
Vol 251 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Jörn A. Holme ◽  
Gunnar Brunborg ◽  
Jan Alexander ◽  
Bente Trygg ◽  
Erik J. Søderlund
Keyword(s):  
Alkaline Elution ◽  
Genotoxic Effects

scholarly journals DNA ligation in relation to DNA strand breaks in phytohemagglutinin- stimulated blast cells from acute lymphoblastic and nonlymphoblastic leukemia

Blood ◽  
1988 ◽  
Vol 72 (2) ◽  
pp. 648-654 ◽  
Author(s):  
SA Feon ◽  
RM Valerius ◽  
NM Genetet ◽  
I Bernard-Griffiths ◽  
PY Le Prise ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Lymphoblastic Leukemia ◽  
Dna Ligase ◽  
Dna Breaks ◽  
Alkaline Elution ◽  
Exogenous Dna ◽  
Strand Breaks ◽  
Dna Ligation ◽  
Blast Cells ◽  
Dna Strand

DNA ligase activity was determined in the WBCs from 306 cases of acute lymphoblastic leukemia (ALL) and acute nonlymphocytic leukemia (ANLL). In T-ALL cells this activity was either low or absent. DNA analysis by nucleoid, alkaline elution, and alkaline sucrose centrifugation after cells were embedded in agarose inserts has shown more DNA breaks in T- ALL than in ANLL blasts. Phytohemagglutinin stimulation of T-ALL blasts resulted in the apparent joining of the DNA breaks. Apparent identical results can be obtained by the incubation of DNA with exogenous DNA ligase. The authors suggest that this enzyme is a crucially regulated step of replication and subsequent proliferation in this type of leukemia.

Differential inactivation of three bacteriophages by acid and alkaline pH used in the membrane adsorption–elution method of virus recovery

1980 ◽  
Vol 26 (12) ◽  
pp. 1403-1407 ◽  
Author(s):  
Carolyn M. Sabatino ◽  
Siegfried Maier
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Differential Susceptibility ◽  
Alkaline Ph ◽  
Alkaline Elution ◽  
Adsorption Method ◽  
Acid Ph ◽  
Elution Method ◽  
Virus Recovery ◽  
General Method

The study was prompted by our inability to concentrate phages by a membrane adsorption method effective for polioviruses. Consequently two coliphages, WPK and T4, and F116 of Pseudomonas aeruginosa were tested for their resistance to acid (pH 5.2–3.2) and alkaline (pH 10–11.5) exposures. Only T4 proved acid resistant, allowing for acid adsorption, and only WPK was sufficiently alkaline resistant to allow for alkaline elution. Thus, the differential susceptibility of various phages precludes the use of the acid membrane adsorption–alkaline elution method as a general method for the concentration of phages from large volumes of water.

Nonradiometric Detection of DNA Crosslinks in Mussel Hemolymph by Alkaline Elution

1993 ◽  
Vol 212 (2) ◽  
pp. 402-406 ◽  
Author(s):  
R. Batel ◽  
M. Vukmirovic ◽  
N. Bihari ◽  
R.K. Zahn ◽  
W.E.G. Muller
Keyword(s):  
Alkaline Elution ◽  
Dna Crosslinks

The effect of anoxia on anthracycline-induced DNA damage in the RPMI-6410 human lymphoblastoid cell line

1982 ◽  
Vol 60 (9) ◽  
pp. 873-876 ◽  
Author(s):  
L. Brox ◽  
B. Gowans ◽  
R. To ◽  
A. Belch
Keyword(s):  
Cell Line ◽  
Lymphoblastoid Cell Line ◽  
Dna Strand Breaks ◽  
Primary Role ◽  
Alkaline Elution ◽  
Strand Breaks ◽  
Human Lymphoblastoid Cell ◽  
Strand Breakage ◽  
Human Lymphoblastoid Cell Line ◽  
Dna Strand

The alkaline elution procedure developed by Kohn and co-workers was used with the RPMI-6410 cultured human lymphoblastoid cell line to examine the hypothesis that anthracycline-induced DNA strand scission is mediated by oxygen- or superoxide-derived free radicals. Hypoxia was induced by gassing with nitrogen containing 5% carbon dioxide and less than 4 ppm oxygen. Alkaline elution studies showed hypoxia was induced, as the oxygen enhancement ratios for DNA strand breaks was 2.4 and 2.6 for the 250 R ± oxygen and the 500 R ± oxygen (1 R = 2.58 × 10−4 C/kg) experiments, respectively. The pattern of adriamycin-induced DNA strand breaks and cross-linking was not affected by hypoxia with 1-h adriamycin exposures between 0.05 and 1.0 μg/mL. Similarly, 1-h exposures of N-trifluoroacetyladriamycin-14-valerate at 3 or 10 μg/mL gave essentially identical alkaline elution profiles in the presence or absence of oxygen. These results do not support the hypothesis that oxygen-derived radicals play a primary role in anthracycline-induced DNA strand breakage.

Sign in / Sign up

Export Citation Format

Share Document