Rapid liquid chromatographic determination of tryptophan, tyrosine, 5-hydroxyindoleacetic acid and homovanillic acid in cerebrospinal fluid

1979 ◽  
Vol 164 (4) ◽  
pp. 501-505 ◽  
Author(s):  
George M. Anderson ◽  
J.Gerald Young ◽  
Donald J. Cohen
Keyword(s):  
Cerebrospinal Fluid ◽  
Homovanillic Acid ◽  
Chromatographic Determination ◽  
Hydroxyindoleacetic Acid ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

A new derivative for the gas-liquid chromatographic determination of homovanillic acid

1972 ◽  
Vol 47 (2) ◽  
pp. 592-600 ◽  
Author(s):  
Stanley W. Dziedzic ◽  
Laura M. Bertani ◽  
Donald D. Clarke ◽  
Stanley E. Gitlow
Keyword(s):  
Homovanillic Acid ◽  
Chromatographic Determination ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Simultaneous liquid-chromatographic determination of urinary vanillylmandelic acid, homovanillic acid, and 5-hydroxyindoleacetic acid.

1988 ◽  
Vol 34 (12) ◽  
pp. 2504-2506 ◽  
Author(s):  
A Gironi ◽  
G Seghieri ◽  
M Niccolai ◽  
P Mammini
Keyword(s):  
Homovanillic Acid ◽  
Clinical Laboratory ◽  
Chromatographic Determination ◽  
Gradient Elution ◽  
Vanillylmandelic Acid ◽  
Liquid Chromatographic Method ◽  
Analytical Recovery ◽  
Hydroxyindoleacetic Acid ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We describe a liquid-chromatographic method for quantifying, simultaneously by a single procedure, vanillylmandelic acid (VMA), homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5-HIAA) in urine. After solvent extraction of acidified urine, the analytes were chromatographed on a C8 column, with use of a mobile phase of phosphate buffer (20 mmol/L, pH 4.0) and methanol with a variable gradient elution, and detected fluorometrically. We report the analytical recovery, sensitivity, precision, working linear range, and potential for interference from similar molecules or drugs. The results of such tests demonstrate that the proposed method is sensitive and reproducible. It is, furthermore, easy to perform, and thus is suitable for use in the clinical laboratory.

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