Electrochemical determination of 2,6-diisopropylphenol after high-performance liquid chromatography of extracts from serum

1990 ◽  
Vol 526 ◽  
pp. 293-295 ◽  
Author(s):  
R.A. Eubel ◽  
C.A. Wium ◽  
A.O. Hawtrey ◽  
J. Coetzee
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Electrochemical Determination

Liquid-chromatographic determination of 5-S-L-cysteinyl-L-dopa with electrochemical detection in urine prepurified with a phenylboronate affinity gel.

1983 ◽  
Vol 29 (12) ◽  
pp. 2031-2034 ◽  
Author(s):  
B Kågedal ◽  
A Pettersson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Electrochemical Determination ◽  
Chromatographic Separations ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A phenylboronate affinity gel has been investigated for use in the prepurification of urine before "high-performance" liquid chromatography and electrochemical determination of 5-S-L-cysteinyl-L-dopa. At pH 5.6 this naturally occurring 5-S-cysteinyldopa was adsorbed on a phenylboronate column and was quantitatively eluted with trichloroacetate, pH 3.0. This pretreatment of urine before "high-performance" liquid chromatography produced satisfactory chromatographic separations, and the results were further improved when the purification procedure also included treatment on a cation exchanger. By using 5-S-D-cysteinyl-L-dopa--a diastereomer to 5-S-L-cysteinyl-L-dopa--as an internal standard, we have developed a practical routine method for the quantitative determination of urinary 5-S-L-cysteinyl-L-dopa. The precision (CV = 2.4%) and analytical recovery (96.9%, SD 5.3%) were satisfactory and the results obtained correlated well with a previously described method.

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