Thiopurine methyltransferase activity: new high-performance liquid chromatographic assay conditions

1997 ◽  
Vol 700 (1-2) ◽  
pp. 275-277 ◽  
Author(s):  
Y. Medard ◽  
S. Nafa ◽  
E. Jacqz-Aigrain
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Thiopurine Methyltransferase ◽  
Methyltransferase Activity ◽  
Liquid Chromatographic ◽  
Assay Conditions

scholarly journals Validated High Performance Liquid Chromatographic Quantification of Pamidronate in Bulk and Dosage Form

2020 ◽  
pp. 1-6
Author(s):  
Sherif A. Abdel- Gawad ◽  
Aymen Khalid Al Suwailem ◽  
Sami Salem AlShowiman
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Dosage Forms ◽  
Match Method ◽  
Mobile Phases ◽  
Validation Parameters ◽  
Chromatographic Detection ◽  
Liquid Chromatographic ◽  
Assay Conditions

Aims: The studied drug is lacking the presence of chromophore so a reaction with NBD-Cl is optimized to facilitate its chromatographic detection, so the main aim of the work is to quantify pamidronate in a sensitive and accurate way either in bulk or dosage forms. Methodology: The quantification of this group of drugs is a challenging task as they lack the presence of chromophore groups in their structure. The proposed method depends on the chromatographic quantification of the studied drug after its derivatization via its reaction with 4-Chloro-7-nitro-2,1,3-benzoxazole and the product is separated on ODS C18 column (5 µm, 15 cm x 5 mm, i.d.) as a stationary phase and methanol : water (8:2, v/v) as a mobile phase. The flow rate was 1 ml/min. Results: The studied drug can be determined in the range of 900 - 3000 ng/mL after optimizing the assay conditions to get optimum stationary – mobile phases match. Method validation is performed according to USP-guidelines and different validation parameters like, linearity, accuracy, precision and robustness are calculated and found to be excellent. Conclusion: The proposed method is accurate, sensitive and can be applied for the routine analysis of pamidronate in quality control laboratories.

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