Disposable, enzymatically modified printed film carbon electrodes for use in the high-performance liquid chromatographic–electrochemical detection of glucose or hydrogen peroxide from immobilized enzyme reactors

1998 ◽  
Vol 707 (1-2) ◽  
pp. 3-8 ◽  
Author(s):  
P.G Osborne ◽  
K Yamamoto
Keyword(s):  
Hydrogen Peroxide ◽  
Electrochemical Detection ◽  
High Performance ◽  
Immobilized Enzyme ◽  
High Performance Liquid Chromatographic ◽  
Carbon Electrodes ◽  
Enzyme Reactors ◽  
Immobilized Enzyme Reactors ◽  
Liquid Chromatographic

Simple, sensitive assay of polyamines by high-performance liquid chromatography with electrochemical detection after post-column reaction with immobilized polyamine oxidase.

1989 ◽  
Vol 35 (8) ◽  
pp. 1694-1696 ◽  
Author(s):  
K Maruta ◽  
R Teradaira ◽  
N Watanabe ◽  
T Nagatsu ◽  
M Asano ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Electrochemical Detection ◽  
High Performance ◽  
Immobilized Enzyme ◽  
Platinum Electrode ◽  
Reversed Phase ◽  
Polyamine Oxidase ◽  
Soybean Seedlings ◽  
Liquid Chromatographic ◽  
Urinary Polyamines

Abstract This simple, rapid liquid-chromatographic assay of urinary polyamines (putrescine, spermidine, spermine, and cadaverine) involves electrochemical detection with a post-column immobilized enzyme, polyamine oxidase (EC 1.4.3.6) from soybean seedlings. Polyamines are separated by isocratic ion-pairing reversed-phase chromatography, then enzymatically converted, with release of hydrogen peroxide, via the post-column reactor with immobilized polyamine oxidase; the hydrogen peroxide is detected by electrochemical oxidation on a platinum electrode. The detection limits for injected putrescine, spermidine, and spermine were 0.3, 0.5, 0.6, and 4 pmol, respectively, with linear ranges of two to three orders of magnitude. Reproducibility was also good, with CV values less than 7%. The efficiency of the immobilized enzyme column was not decreased after analysis of 300 urine samples. Putrescine and spermidine excretion in urine from patients with blood cancers and solid cancers was significantly increased.

Ascorbate in plasma as measured by liquid chromatography and by dichlorophenolindophenol colorimetry.

1986 ◽  
Vol 32 (6) ◽  
pp. 1004-1006 ◽  
Author(s):  
D J VanderJagt ◽  
P J Garry ◽  
W C Hunt
Keyword(s):  
Ascorbic Acid ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Colorimetric Method ◽  
Dehydroascorbic Acid ◽  
High Performance Liquid Chromatographic ◽  
Plasma Samples ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract Ascorbic acid was measured in 125 plasma samples by an automated colorimetric method involving dichlorophenolindophenol and by a "high-performance" liquid-chromatographic procedure with electrochemical detection. The two methods gave comparable results for samples with ascorbate concentrations of 1 to 20 mg/L (r = 0.97). We also measured the amount of total ascorbate (ascorbic acid + dehydroascorbic acid) in the same samples by a liquid-chromatographic procedure with precolumn derivitization of ascorbic acid. We confirmed that plasma contains little dehydroascorbic acid.

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