Guidelines for the characterization of immobilized cells

Author(s):  
R.H. Wijffels ◽  
R.M. Buitelaar ◽  
C. Bucke ◽  
P.B. Poulsen ◽  
M.D. Lilly ◽  
...  
Keyword(s):  
1996 ◽  
Vol 34 (10) ◽  
pp. 51-58 ◽  
Author(s):  
Chen Wen-Chin ◽  
Cheng Sheng-Shung

Because of the presence of refractory and biologically inhibitory organic compounds in ABS resin wastewater, which would retard the bioactivity of the autotrophic nitrifier and make the efficiency of nitrification process unstable, the application of cell immobilization for ABS wastewater treatment has been investigated by immobilizing the enrichment culture of nitrifying bacteria with alginate. The characterization of the immobilized cells in various alignate concentrations was conducted by a series of biodegradability studies with electrolytic respirometry and compared with the differences from the dispersed cells. Results clearly indicated that immobilized cells achieved higher efficiency of COD and TKN removal than that of the dispersed cells during the period of high initial concentration of wastewater. Meanwhile, high concentration of cells were growing near the gel surface and reduced the effects of diffusional restriction. No remarkable difference was found while the concentration of alginate ranged from 3% to 5%. According to mass balance of total nitrogen, more than 25% of total nitrogen was removed in the batch system of the immobilized cell. Simultaneous nitrification and denitrification could occur due to the oxygen gradient throughout the gel matrix. Compared with the complete mixing system of the dispersed cell, the approach for the immobilized-cell system provided a more effective method for treating the special wastewater with high strength of nitrogenous and inhibitory compounds.


2010 ◽  
Vol 48 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Haroldo Y. Kawaguti ◽  
Érica M. Celestino ◽  
Ana L.L. Moraes ◽  
Dong K. Yim ◽  
Larissa K. Yamamoto ◽  
...  
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