Characterization of the full length uracil-DNA glycosylase in the extreme thermophile Thermotoga maritima

2001 ◽  
Vol 485 (3) ◽  
pp. 187-195 ◽  
Author(s):  
Margarita Sandigursky ◽  
Alexander Faje ◽  
William A. Franklin
Keyword(s):  
Thermotoga Maritima ◽  
Full Length ◽  
Dna Glycosylase ◽  
Extreme Thermophile ◽  
Uracil Dna Glycosylase

Characterization of GM-CSF-inhibitory factor and Uracil DNA glycosylase encoding genes from camel pseudocowpoxvirus

2015 ◽  
Vol 100 ◽  
pp. 291-296 ◽  
Author(s):  
G. Nagarajan ◽  
Shelesh Kumar Swami ◽  
Shyam Singh Dahiya ◽  
S.D. Narnaware ◽  
S.C. Mehta ◽  
...  
Keyword(s):  
Inhibitory Factor ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Gm Csf ◽  
Encoding Genes

Characterization of cold-active uracil-DNA glycosylase from Bacillus sp. HJ171 and its use for contamination control in PCR

2008 ◽  
Vol 80 (5) ◽  
pp. 785-794 ◽  
Author(s):  
Gun A. Kim ◽  
Mi Sun Lee ◽  
Younguk Sun ◽  
Byung Doo Lee ◽  
Jong Il Lee ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Bacillus Sp ◽  
Uracil Dna Glycosylase ◽  
Contamination Control ◽  
Cold Active

scholarly journals Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins

2005 ◽  
Vol 387 (3) ◽  
pp. 859-863 ◽  
Author(s):  
Isabelle DIONNE ◽  
Stephen D. BELL
Keyword(s):  
Protein S ◽  
Dna Glycosylase ◽  
Nuclear Antigen ◽  
Uracil Dna Glycosylase ◽  
Sliding Clamp ◽  
C Terminus ◽  
Marked Preference ◽  
Chromatin Proteins ◽  
Mechanistic Basis

We describe the characterization of a family 4 UDG1 (uracil DNA glycosylase) from the crenarchaeote Sulfolobus solfataricus. UDG1 is found to have a marked preference for substrates containing a G:U base pair over either A:U or single-stranded uracil-containing DNA substrates. UDG1 is found to interact with the sliding clamp PCNA (proliferating cell nuclear antigen), and does so by a conserved motif in the C-terminus of the protein. S. solfataricus has a heterotrimeric PCNA, and only one of the subunits, PCNA3, interacts with UDG1. We have been unable to detect any stimulation of UDG activity by PCNA, in contrast with the observed effects of PCNA on a number of DNA metabolic enzymes. However, analysis of the effects of Sulfolobus chromatin proteins on UDG1 leads us to propose a mechanistic basis for coupling UDG1 to the replication fork.

scholarly journals Characterization of a Family IV uracil DNA glycosylase from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5

2020 ◽  
Vol 146 ◽  
pp. 475-481
Author(s):  
Qi Gan ◽  
Mengfan He ◽  
Haoqiang Shi ◽  
Zhihui Yang ◽  
Philippe Oger ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Family Iv
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