Phospholipid hydroperoxide glutathione peroxidase activity and vitamin E level in the liver microsomal membrane: effects of age and dietary α-linolenic acid deficiency

2001 ◽  
Vol 12 (8) ◽  
pp. 481-491 ◽  
Author(s):  
Alain Linard ◽  
Jean-Paul Macaire ◽  
Raymond Christon
Keyword(s):  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Linolenic Acid ◽  
Peroxidase Activity ◽  
Microsomal Membrane ◽  
Phospholipid Hydroperoxide Glutathione Peroxidase ◽  
Glutathione Peroxidase Activity ◽  
Phospholipid Hydroperoxide ◽  
Acid Deficiency ◽  
Membrane Effects

scholarly journals Phospholipid hydroperoxide glutathione peroxidase activity of human glutathione transferases

1998 ◽  
Vol 332 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Rachel HURST ◽  
Yongping BAO ◽  
Per JEMTH ◽  
Bengt MANNERVIK ◽  
Gary WILLIAMSON
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Hplc Method ◽  
Glutathione Transferases ◽  
Phospholipid Hydroperoxide Glutathione Peroxidase ◽  
Glutathione Peroxidase Activity ◽  
Phospholipid Hydroperoxide ◽  
Hydrophobic Site ◽  
Hydrophobic Binding ◽  
Specific Activities

Human glutathione transferases (GSTs) from Alpha (A), Mu (M) and Theta (T) classes exhibited glutathione peroxidase activity towards phospholipid hydroperoxide. The specific activities are in the order: GST A1-1 > GST T1-1 > GST M1-1 > GST A2-2 > GST A4-4. Using a specific and sensitive HPLC method, specific activities towards the phospholipid hydroperoxide, 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-l-3-phosphatidylcholine (PLPC-OOH) were determined to be in the range of 0.8–20 nmol/min per mg of protein. Two human class Pi (P) enzymes (GST P1-1 with Ile or Val at position 105) displayed no activity towards the phospholipid hydroperoxide. Michaelis–Menten kinetics were followed only for glutathione, whereas there was a linear dependence of rate with PLPC-OOH concentration. Unlike the selenium-dependent phospholipid hydroperoxide glutathione peroxidase (Se-PHGPx), the presence of detergent inhibited the activity of GST A1-1 on PLPC-OOH. Also, in contrast with Se-PHGPx, only glutathione could act as the reducing agent for GST A1-1. A GST A1-1 mutant (Arg15Lys), which retains the positive charge between the GSH- and hydrophobic binding sites, exhibited a decreased kcat for PLPC-OOH but not for CDNB, suggesting that the correct topography of the GSH site is more critical for the phospholipid substrate. A Met208Ala mutation, which gives a modified hydrophobic site, decreased the kcat for CDNB and PLPC-OOH by comparable amounts. These results indicate that Alpha, Mu and Theta class human GSTs provide protection against accumulation of cellular phospholipid hydroperoxides.

scholarly journals Effect of vitamin E (DL-all-rac-a-tocopherol acetate) and nano particles of selenium on growth, survival, body composition and whole body glutathione peroxidase (GPX) and malondialdehyde (MDA) in Rutilus kutum (Kamensky, 1901)

2017 ◽  
Vol 19 (2) ◽  
pp. 69-76
Author(s):  
Davoud Tahmasbi ◽  
Saeid Gorgin ◽  
Mohammad Mazendarani ◽  
Mohammad Sudagar
Keyword(s):  
Body Composition ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Carcass Composition ◽  
Nano Particles ◽  
Whole Body ◽  
Glutathione Peroxidase Activity ◽  
Tocopherol Acetate ◽  
Malondialdehyde Content

Abstract The effect of vitamin E (100 mg kg−1) and nano-selenium (1 mg kg−1), which have a nutritional relationship separately and in combination, was investigated on growth, survival, carcass composition, body glutathione peroxidase activity, and body malondialdehyde content of Rutilus kutum. Results showed that vitamin E is capable of improving growth, FCR and WG in Kutum fingerlings; however, nano-selenium is not. According to this study, vitamin E can improve growth and selenium can improve glutathione peroxidase activity in Rutilus kutum larvae.

0818 Effect of selenium and vitamin E supplementation on blood glutathione peroxidase activity and selenium in moderately exercised horses

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 393-394
Author(s):  
E. Velázquez Cantón ◽  
A. H. Ramírez Pérez ◽  
L. A. Zarco Quintero ◽  
R. Rosiles Martínez ◽  
J. C. Ángeles Hernández
Keyword(s):  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Glutathione Peroxidase Activity ◽  
Vitamin E Supplementation

Vitamin E protection of obesity-enhanced vascular calcification in uremic rats

2014 ◽  
Vol 306 (4) ◽  
pp. F422-F429 ◽  
Author(s):  
A. Peralta-Ramírez ◽  
A. Montes de Oca ◽  
A. I. Raya ◽  
C. Pineda ◽  
I. López ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Calcium Concentration ◽  
Soft Tissues ◽  
Zucker Rats ◽  
Glutathione Peroxidase Activity ◽  
Obese Rats

This study aimed to determine the extent of extraskeletal calcification in uremic Zucker rats, by comparing obese and lean phenotypes, and to evaluate the influence of vitamin E (VitE) on the development of calcifications in both uremic rats and human vascular smooth muscle cells (HVSMCs) cultured in vitro. Zucker rats of lean and obese phenotypes with normal renal function [control (C); C-lean and C-obese groups] and with uremia [5/6 nephrectomy (Nx); Nx-lean and Nx-obese groups] and uremic rats treated with VitE (Nx-lean + VitE and Nx-obese + VitE groups) were studied. Uremic groups were subjected to Nx, fed a 0.9% phosphorus diet, and treated with calcitriol (80 ng/kg ip). The aortic calcium concentration was significantly higher ( P < 0.05) in Nx-obese rats (10.0 ± 2.1 mg/g tissue) than in Nx-lean rats (3.6 ± 1.3 mg/g tissue). A decrease in plasma glutathione peroxidase activity was observed in Nx-obese rats compared with Nx-lean rats (217.2 ± 18.2 vs. 382.3 ± 15.5 nmol·min−1·ml−1, P < 0.05). Treatment with VitE restored glutathione peroxidase activity and reduced the aortic calcium concentration to 4.6 ± 1.3 mg/g tissue. The differences in mineral deposition between Nx-lean, Nx-obese, Nx-lean + VitE, and Nx-obese + VitE rats were also evidenced in other soft tissues. In HVSMCs incubated with high phosphate, VitE also prevented oxidative stress and reduced calcium content, bone alkaline phosphatase, and gene expression of core-binding factor-α1. In conclusion, uremic obese rats develop more severe calcifications than uremic lean rats and VitE reduces oxidative stress and vascular calcifications in both rats and cultures of HVSMCs.

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