Limiting amino acids supplementation in low crude protein diets and their impacts on growth performance and carcass composition in Labeo rohita (rohu) adult fish

Ninety days study was conducted in hapas installed in earthen ponds. Fish of an average initial weight (220g) were evenly distributed in triplicate groups within fifteen hapas. Five experimental diets labeled as T1 (25% CP and NRC recommended amino acid level) as control diet, T2 (with 2% low protein and 5% amino acid supplementation), T3 (with 2% low protein and 10% amino acid supplementation), T4 (with 4% low protein and 10% amino acid supplementation) and T5 (with 4% low protein and 20% amino acid supplementation) were prepared. Fish were fed with @3% of their body weight twice a day at 10.00 & 16:00 hour. Significantly higher percent weight gain (420.18 ± 66.84a) and specific growth rate (13499.33±1273.54a) along with improved feed conversion ratio (1.29 ± 0.09b) and hundred percent survivals were recorded during the trial. Furthermore proximate analysis of meat showed significant improvement in the crude protein level (81.77 ± 0.19a) served with diet containing 20% limiting amino acids mixture. Therefore, limiting amino acids can be a source of cost effective feed and use safely in L. rohita diet.

Use of multi-strain probiotics in linseed meal based diet for Labeo rohita fingerlings

Unavailability of probiotics in fish digestive system fingerlings is unable to digest and absorb their food properly. The current research was conducted to investigate the influence of probiotics added Linseed meal based (LMB) diet on hematology and carcass composition of Labeo rohita juveniles. Hematological parameters are essential diagnostics used to estimate the health status of fish. The usage of probiotics for fish health improvement is becoming common due to the higher demand for environment-friendly culture system in water. Linseed meal was used as a test ingredient to prepare six experimental test diets by adding probiotics (0, 1, 2, 3, 4 and 5 g/kg) and 1% indigestible chromic oxide for seventy days. According to their live wet weight, five percent feed was given to fingerlings twice a day. Fish blood and carcass samples (Whole body) were taken for hematological and carcass analysis at the end of the experiment. The highest carcass composition (crude protein; 18.72%, crude fat; 8.80% and gross energy; 2.31 kcal/g) was observed in fish fed with test diet II supplemented with probiotics (2 g/kg). Moreover, maximum RBCs number (2.62× 106mm-3), WBCs (7.84×103mm-3), PCV (24.61), platelets (63.85) and hemoglobin (7.87) had also been reported in the fish fingerlings fed on 2 g/kg of probiotics supplemented diet. Results indicated that probiotics supplementation has a critical role in improvement of fingerlings’ body composition and hematological indices. Present findings showed that probiotics supplementation at 2 g/kg level in linseed by-product-based diet was very useful for enhancing the overall performance of L. rohita fingerlings.

Replacement level of rubber seed cake for soybean meal on the growth of Japanese quail

A four-week feeding trial on the simultaneous replacement of 0 to 50% of soybean meal and 0 to 100% of soybean oil on the growth performance, carcass composition and profitability was conducted using 45 randomly chosen one-week-old growing Japanese quail. The five experimental diets were; D0= Diet containing 20% soybean meal and 4% soybean oil (control diet), D1= Diet, where 12.5% of soybean meal and 25% of soybean oil has been replaced by RSC; T2 = Diet, where 25% of soybean meal and 50% of soybean oil has been replaced by RSC; T3 = Diet, where 37.5% of soybean meal and 75% of soybean oil has been replaced by RSC; T4 = Diet, where 50% of soybean meal and 100% of soybean oil has been replaced by RSC. Results demonstrated that in raw rubber seed the proportion of kernel to hull is 64: 36. Proximate components of the boiled and sundried full fat rubber seed kernel (RS) were, moisture = 96.6%, ME = 5305 kcal / kg DM, CP = 17.6%, EE = 51.05%, CF = 8.5%, NFE = 18.25% and Ash = 4.6%. The body weights of the birds at 35 days of age in all dietary treatments were statistically similar. The feed intake of the quail among different dietary groups was varied significantly (p < 0.05) and there was a tendency of decreasing the feed intake at 25% and above inclusion level of RSC. The body weight gain and FCR were also statistically similar in all dietary groups. The feed cost and total production cost /kg BW of quail was also found to become steadily higher (p> 0.05) at higher RSC inclusion level. The muscle development was found to better at lower (%) RSC level, whereas the organs were enlarged at higher RSC inclusion. Results on the majority of the production parameters, and economics and carcass yield parameters suggested that the simultaneous replacement of maximum 50% soybean oil 25% soybean meal by processed RSC might be suggested, particularly, there will be a crisis in the availability of these two ingredients.

Growth Performance and Carcass Analysis of African Catfish (Clarias Gariepinus) Juveniles Fed Varying Inclusion Levels Of Butterfly Pea (Clitoria Ternatea) Seed Meal

High cost of qualitative fish feed is one of problems hampering the development and profitability of fish farming and consequently leads to food insecurity in terms of food availability and accessibility. The study was conducted to determine growth parameters and carcass composition of Clarias gariepinus juveniles of mean weight 21.74g+0.54g fed with varying levels of dietary inclusion of Clitoria ternatea seed meal substituted for soybean meal over a period of eight weeks. Four iso-nitrogenous experimental diets were formulated at 0% (control), 25%, 50% and 100% inclusion levels of C. ternatea seed meal. The 8-week feeding experiment was conducted in rectangular plastic tanks, each treatment having three replicates with two feeding regime at 5% body weight and weighed every week. At the end of the intensive feeding period, samples from each treatment were oven dried for nutritive analysis of the fish carcass. The values of mean weight gain for diets I and III were 27.7+1.11 and 27.23+0.94 respectively. Comparatively, the growth parameters values; FI(g), SGR(%), FCR(g), PER(%) and NPU(%) for control diet and diet III were the best with no significant difference (p>0.05). The Carcass evaluation revealed that Diet I had the highest crude protein which was not significantly different (p>0.05) from diet III. It was established that fish fed 50% inclusion level of C. ternatea seed meal had effective comparable biological values with the control diet in terms of growth performance and feed utilization. The total fish production (kg) showed no significant difference (p>0.05) between diets I and III.

EFFECTS OF SLAUGHTER’ AGE ON GROWTH PERFORMANCE, CARCASS AND NON-CARCASS COMPONENTS TRAITS OF BERBER BREED LAMBS

The objective of this work was to study the lamb’s growth, the carcass yield and composition and the importance of main organs of Berber lambs slaughtered at 4 or 6 months. The study concerned 40 lambs reared under motherhood. Lambs of the first group were slaughtered at the age of 4 months (4M) and the second at 6 months (6M). Slaughter body weights (BW), hot and cold carcass weights and organs were recorded. The carcass was cut out and dissected. The average BW at birth was 4.14 + 0.506 kg while the BW at slaughter was 22.86 kg + 3.40 and 31.51 + 3.50 kg for 4M and 6M groups, respectively. The average daily gain were 172 and 164g for 4M and 6M groups, respectively. Therefore, during 2months the lambs of 6M group gained 8.67 kg in BW. The carcasses were heavier for 6M than 4M. The carcass commercial yields were 42% and 45% for 4M and 6M groups, respectively, resulting in 4.1 kg gain of meat during 2 months. The tissular (muscle, fat and bone) and the regional (leg, shoulder …) carcass composition did not differ among groups. All organs were heavier for 6M than 4M; however, their proportion in the empty body weight were variables. The BWand carcass gain at six months are encouraging to prolong the lamb’s slaughter age without carcass composition difference given the similar fat proportion in the carcass of both groups.

Mannan Oligosaccharide Enhanced the Growth Rate, Digestive Enzyme Activity, Carcass Composition, and Blood Chemistry of Thinlip Grey Mullet (Liza ramada)

Mannan oligosaccharide (MOS) is prebiotic with high functionality in aquaculture. The current study investigated the potential roles of MOS on the growth performance, digestive enzyme activity, carcass composition, and blood chemistry of Thinlip grey mullet (Liza ramada). Four tested diets with 34.49% crude protein and 6.29% of total lipids were prepared and fortified with 0, 0.5, 1, and 2% MOS. Fish of initial weight = 5.14 ± 0.11 g/fish were distributed in 12 hapas (0.5 × 0.5 × 1 m) at 15 fish per hapa (triplicates) and fed the test diets to the satiation level two times a day (08:00 and 15:00) for eight weeks. At the end of the trial, all fish were weighed individually for growth performance calculation. Blood was collected to check blood chemistry traits, and intestines were dissected for digestive enzyme analysis. Fish treated with MOS had marked enhancement in the final body weight, feed conversion ratio, protein gain, and protein retention regardless of inclusion dose (p < 0.05). The weight gain, specific growth rate, and protein efficiency ratio were meaningfully enhanced by including MOS at 0.5 and 1%, followed by fish fed with 2% MOS, while the lowest values were in the control group (p < 0.05). Insignificant influences of MOS were seen on the chemical composition of carcass components (moisture, crude protein, total lipids, and ash) (p > 0.05). Fish treated with MOS at 0.5 and 1% had marked enhancement in the amylase, lipase, and protease activities regardless of inclusion dose (p < 0.05). The blood total protein and albumin levels were meaningfully enhanced by including MOS at 0.5 and 1%, followed by fish fed with 2% MOS, while the lowest values were in the control group (p < 0.05). The blood globulin was significantly enhanced in fish fed 1% MOS than fish treated with 0, 0.5, and 2% of MOS (p < 0.05). The blood lysozyme activity was meaningfully enhanced by including MOS at 1%, followed by fish treated with 0.5 and 2%, while the lowest values were in the control group (p < 0.05). Phagocytic activity and phagocytic index were markedly improved in fish treated with 1 and 2% MOS, followed by those fed 0.5% compared with fish fed MOS-free diet (p < 0.05). Superoxide dismutase and glutathione peroxidase were markedly improved in fish treated with 1, and 2% MOS, followed by those fed 0.5% compared with fish fed MOS-free diet (p < 0.05). Dietary MOS (0.5, 1, and 2%) meaningfully enhanced catalase activity while decreased the malondialdehyde concentration (p < 0.05). In summary, dietary MOS is required at 0.5–1% for enhancing the growth rate, feed efficiency, digestive enzyme activity, blood chemistry, and antioxidative capacity of grey mullet.

Effect of Changes in Dietary Net Energy Concentration on Growth Performance, Fat Deposition, Skatole Production, and Intestinal Morphology in Immunocastrated Male Pigs

Nutritional requirements of heavy immunocastrated (IM) pigs and therefore appropriate feeding strategies have not yet been determined. Thus, the effects of changes in dietary net energy (NE) content were studied in 41 IM pigs, fed ad libitum diets with low, medium, and high NE content (LNE, MNE, and HNE diets, with 8.5, 9.3, and 10.0 MJ NE/kg, respectively), from 84 days of age until slaughter at an average age of 172 days and an average body weight of 122.5 kg. In the period from 143 to 170 days of age, there was a tendency for a greater NE intake (p = 0.08) in pigs fed the HNE diet along with greater (p &lt; 0.01) backfat gain. Dietary treatment affected carcass composition, as lower backfat thickness (p = 0.01) and lower area of fat over the longissimus muscle (p = 0.05) were observed in the LNE and MNE pigs. In addition, greater lean meat content (p = 0.04) was observed in the LNE pigs. Reducing the NE of the diet by replacement of cereals and soybean meal with high-fiber ingredients resulted in lower indole production in the ascending colon (p &lt; 0.01) and greater skatole production (p &lt; 0.01) in the cecum. Greater villus area, width, height and perimeter, crypt depth, and thickness of the intestinal mucosa in the jejunum, ileum, ascending colon, and descending colon were found in the LNE group (p &lt; 0.01) than in the HNE group, while those in the MNE group was intermediate. Cell proliferation was not affected by dietary treatment (p &gt; 0.05). The present results show that a reduction in dietary NE concentration lowers lipid deposition, without affecting performance or energy efficiency in IM pigs. This technique provides an advantage in terms of improved leanness, without affecting growth rate in IM pigs after immunization, which is particularly important when the backfat thickness is a determinant of carcass value and IM pigs are fattened to higher weights (e.g., in heavy pig production) or when a longer delay between immunization and slaughter is practiced.

Performance and blood lipide profile of cockerel aged 0-7 weeks that were given sambiloto leaf water extract (Andrographis paniculata ness) through drinking water

This study aims to examine the effect of adding water extract of Sambiloto (Andrographis paniculata Ness) to drinking water on the performance and blood lipids profilem of cockerel aged 0-7 weeks. This study was conducted in the village of Cepaka, Kediri, Tabanan, Bali. Using A total of 200 birds of aged 1 day old chick laying hens males.. The experimental design used was a Completely Randomized Design (CRD) with four treatments and five replications so that there were 20 units, each replicate using 10 chickens. The treatments were: (A) Drinking water without the addition of extract sambiloto leaf water, (B) drinking water with the addition of sambiloto leaf water extract 2 ml/l , (C) drinking water with the addition of sambiloto leaf water extract 4 ml/l and (D) drinking water with the addition of sambiloto leaf water extract 6 ml/L The variables observed in this study were performance, carcass, blood lipid profile, digestive tract microbes and economic aspec. The results showed that the administration of sambiloto leaf water extract not significantly different (P>0.05) on ration consumption, drinking water consumption, final body weight, FCR, blood lipid profile, total coliform and E.coly and significantly different (P<0.05) on carcass percentage, carcass composition.

Carcass Composition and Quality of Meat of Pulawska and Pulawska x PLW Crossbred Pigs Fed Rations with Naked Oats

The study aimed to determine the impact of a 60% inclusion of naked oats in feed rations for Pulawska pigs and Pulawska x Polish Large White (PLW) crossbreds on the fattening performance, carcass composition, and meat quality. It was demonstrated that—independent from their diet—Pulawska pigs showed a daily weight gain about 14.5% lower (p ≤ 0.05), with about 15% higher feed conversion rate. The experimental factors showed no impact on the dressing percentage, meatiness, and backfat thickness, except that the “eye” of the loin was significantly smaller (by 4.55 cm2) in Pulawska pigs. There was no interaction effect (diet/breed) for the pigs’ performance results and carcass composition. A 60% inclusion of naked oats in the pigs’ diet did not affect the weight of primal cuts in the right-side half-carcass. Compared to Pulawska pigs, crossbreds featured a significantly lower weight of bacon and ribs (by 1.17 kg) but a higher (p ≤ 0.05) weight of fillet (by 1.0 kg) and ham with shin (by 0.43 kg). The diet had no impact on the evaluated muscles, except a reduction (by 2.3 points) in colour lightness (L) of musculus semimembranosus in fattening pigs receiving feed rations with a 60% share of oats. Both muscles in Pulawska pigs showed significantly better (p ≤ 0.05) water-holding capacity and reduced colour lightness (L) in comparison to crossbred pigs. Moreover, the longissimus lumborum muscle of Pulawska pigs had a higher chroma (C) and a lower hue (H). The contents of essential nutrients in the evaluated muscles did not depend on the pigs’ diet and breed, except that a higher by 0.3% (p ≤ 0.05) intramuscular fat content was found in the longissimus lumborum muscle of Pulawska pigs. Neither of the experimental factors showed significant impact on the total saturated fatty acids (SFA) and total unsaturated fatty acids (UFA), or on neutral or hypocholesterolemic (DFA) and hypercholesterolemic fatty acids (OFA) in the evaluated muscles. The muscles of Pulawska x PLW pigs contained more (by 1.77% FA in the longissimus lumborum and 1.16% in the semimembranosus) polyunsaturated fatty acids (PUFA) than the muscles of the Pulawska breed (p ≤ 0.05). In addition, naked oats included in the pigs’ diet increased (p ≤ 0.05) the share of PUFA in intramuscular fat. The muscles of Pulawska pigs, in comparison to the muscles of crossbreds, showed a significantly improved ratio: by 66% and 69% in the longissimus lumborum and the semimembranosus muscles, respectively. To sum up, a 60% inclusion of naked oats is recommended in the rations of both Pulawska pigs and Pulawska x PLW crossbreds, since it allows satisfactory carcass composition and meat quality to be maintained. Pulawska pigs had worse productivity ratios but showed improved meat quality traits in terms of the n-6/n-3 ratio.