Molecular analysis of a spontaneous insertion mutation near the alcohol dehydrogenase gene of Drosophila melanogaster

Abstract This report describes the structure and expression of the outspread (osp) gene of Drosophila melanogaster. Previous work showed that chromosomal breakpoints associated with mutations of the osp locus map to both sides of the alcohol dehydrogenase gene (Adh), suggesting that Adh and the adjacent gene Adh' are nested in osp. We extended a chromosomal walk and mapped additional osp mutations to define the maximum molecular limit of osp as 119 kb. We identified a 6-kb transcript that hybridizes to osp region DNA and is altered or absent in osp mutants. Accumulation of this RNA peaks during embryonic and pupal periods. The osp cDNAs comprise two distinct classes based on alternative splicing patterns. The 5′ end of the longest cDNA was extended by PCR amplification. When hybridized to the osp walk, the 5′ extension verifies that Adh and Adh' are nested in osp and shows that osp has a transcription unit of ≥74 kb. In situ hybridization shows that osp is expressed both maternally and zygotically. In the ovary, osp is transcribed in nurse cells and localized in the oocyte. In embryos, expression is most abundant in the developing visceral and somatic musculature.

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Molecular analysis of alcohol dehydrogenase electromorphs in wild type and transformed Drosophila melanogaster

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(88)90442-1 ◽

1988 ◽

Vol 150 (2) ◽

pp. 655-664 ◽

Cited By ~ 1

Author(s):

Mark A. Batzer ◽

Trent D. Desselle ◽

Mark D. Brennan ◽

William R. Lee ◽

Bruce Tedeschi

Keyword(s):

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Molecular Analysis ◽

Wild Type

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Restriction endonuclease variation in the region of the alcohol dehydrogenase gene: a comparison of null and normal alleles from natural populations of Drosophila melanogaster

Heredity ◽

10.1038/hdy.1988.15 ◽

1988 ◽

Vol 60 (1) ◽

pp. 101-107 ◽

Cited By ~ 8

Author(s):

Chengshan Jiang ◽

John B Gibson ◽

Ann V Wilks ◽

Allan L Freeth

Keyword(s):

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Restriction Endonuclease ◽

Natural Populations ◽

Dehydrogenase Gene

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Nonsense mutations in the alcohol dehydrogenase gene of Drosophila melanogaster correlate with an abnormal 3′ end processing of the corresponding pre-mRNA

RNA ◽

10.1017/s1355838299981359 ◽

1999 ◽

Vol 5 (4) ◽

pp. 562-573 ◽

Cited By ~ 32

Author(s):

SAVERIO BROGNA

Keyword(s):

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Nonsense Mutations ◽

Dehydrogenase Gene

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UGA nonsense mutation in the alcohol dehydrogenase gene of Drosophila melanogaster

Journal of Molecular Biology ◽

10.1016/0022-2836(85)90375-4 ◽

1985 ◽

Vol 184 (2) ◽

pp. 221-229 ◽

Cited By ~ 18

Author(s):

Presley F. Martin ◽

Allen R. Place ◽

Ellen Pentz ◽

William Sofer

Keyword(s):

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Nonsense Mutation ◽

Dehydrogenase Gene

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Temporal and spatial utilization of the alcohol dehydrogenase gene promoters during the development of Drosophila melanogaster

Developmental Biology ◽

10.1016/0012-1606(89)90115-2 ◽

1989 ◽

Vol 134 (2) ◽

pp. 430-437 ◽

Cited By ~ 10

Author(s):

Trevor J. Lockett ◽

Michael Ashburner

Keyword(s):

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Gene Promoters ◽

Dehydrogenase Gene ◽

Temporal And Spatial ◽

Spatial Utilization

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Structure of the Drosophila melanogaster Glutathione-Dependent Formaldehyde Dehydrogenase/Octanol Dehydrogenase Gene (Class III Alcohol Dehydrogenase). Evolutionary Pathway of the Alcohol Dehydrogenase Genes

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1994.0985b.x ◽

1994 ◽

Vol 225 (3) ◽

pp. 985-993 ◽

Cited By ~ 17

Author(s):

Teresa Luque ◽

Silvia Atrian ◽

Olle Danielsson ◽

Hans Jornvall ◽

Roser Gonzalez-Duarte

Keyword(s):

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Formaldehyde Dehydrogenase ◽

Class Iii ◽

Evolutionary Pathway ◽

Dehydrogenase Gene ◽

Octanol Dehydrogenase

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Insertion of the retroposable element, jockey, near the Adh gene of Drosophila melanogaster is associated with altered gene expression

Genetics Research ◽

10.1017/s0016672300034170 ◽

1996 ◽

Vol 68 (3) ◽

pp. 203-209 ◽

Cited By ~ 2

Author(s):

Lisa D. White ◽

James W. Jacobson

Keyword(s):

Gene Expression ◽

Drosophila Melanogaster ◽

Alcohol Dehydrogenase ◽

Insertion Mutation ◽

Activity Levels ◽

Mrna Levels ◽

Wild Type ◽

Reporter System ◽

Wild Type Counterpart ◽

Altered Gene

SummaryThe alcohol dehydrogenase (Adh) gene of Drosophila melanogaster is well suited to be a gene expression reporter system. Adh produces a measurable phenotype at both the enzyme and mRNA levels. We recovered a spontaneous transposable element (TE) insertion mutation near the Adh gene. The insertion is a truncated retroposable element, jockey, inserted upstream of the adult Adh enhancer region. Comparisons between the Adhjockey allele and its direct wild-type ancestral allele were made in an isogenic background (i.e. identical cis and trans factors). Differences in Adhjockey expression compared with the wild-type can be attributed solely to the presence of the jockey element. This jockey insertion results in a decrease in adult mRNA transcript levels in the Adhjockey homozygous lines relative to the wild-type counterpart and accounts for a correlated decrease in alcohol dehydrogenase (ADH) enzyme activity. The larval ADH activity levels are not detectably different.

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