scholarly journals How a protein generates a catalytic radical from coenzyme B12: X-ray structure of a diol-dehydratase–adeninylpentylcobalamin complex

Structure ◽  
2000 ◽  
Vol 8 (7) ◽  
pp. 775-788 ◽  
Author(s):  
Jun Masuda ◽  
Naoki Shibata ◽  
Yukio Morimoto ◽  
Tetsuo Toraya ◽  
Noritake Yasuoka
Keyword(s):  
Coenzyme B12 ◽  
X Ray ◽  
Diol Dehydratase

scholarly journals Release of a Damaged Cofactor from a Coenzyme B12-Dependent Enzyme: X-Ray Structures of Diol Dehydratase-Reactivating Factor

Structure ◽  
2005 ◽  
Vol 13 (12) ◽  
pp. 1745-1754 ◽  
Author(s):  
Naoki Shibata ◽  
Koichi Mori ◽  
Naoki Hieda ◽  
Yoshiki Higuchi ◽  
Mamoru Yamanishi ◽  
...  
Keyword(s):  
Coenzyme B12 ◽  
X Ray ◽  
Diol Dehydratase

Crystallization and preliminary X-ray study of two crystal forms of Klebsiella oxytoca diol dehydratase–cyanocobalamin complex

1999 ◽  
Vol 55 (4) ◽  
pp. 907-909 ◽  
Author(s):  
Jun Masuda ◽  
Tetsuya Yamaguchi ◽  
Takamasa Tobimatsu ◽  
Tetsuo Toraya ◽  
Kyoko Suto ◽  
...  
Keyword(s):  
Klebsiella Oxytoca ◽  
Crystal Form ◽  
Unit Cell ◽  
Unit Cell Parameters ◽  
Crystal Forms ◽  
Space Group ◽  
X Ray ◽  
Cell Parameters ◽  
Diol Dehydratase ◽  
Replacement Method

Two crystal forms of Klebsiella oxytoca diol dehydratase complexed with cyanocobalamin have been obtained and preliminary crystallographic experiments have been performed. The crystals belong to two different space groups, depending on the crystallization conditions. One crystal (form I) belongs to space group P212121 with unit-cell parameters a = 76.2, b = 122.3, c = 209.6 Å, and diffracts to 2.2 Å resolution using an X-ray beam from a synchrotron radiation source. The other crystal (form II) belongs to space group P21 with unit-cell parameters a = 75.4, b = 132.7, c = 298.8 Å, β = 91.9°, and diffracts to 3.0 Å resolution. For the purpose of structure determination, a heavy-atom derivative search was carried out and some mercuric derivatives were found to be promising. Structure analysis by the multiple isomorphous replacement method is now under way.

Mechanism-based Inactivation of Coenzyme B12-dependent Diol Dehydratase by 3-Unsaturated 1,2-Diols and Thioglycerol

2008 ◽  
Vol 144 (4) ◽  
pp. 437-446 ◽  
Author(s):  
T. Toraya ◽  
N. Tamura ◽  
T. Watanabe ◽  
M. Yamanishi ◽  
N. Hieda ◽  
...  
Keyword(s):  
Coenzyme B12 ◽  
Diol Dehydratase

scholarly journals Structural Rationalization for the Lack of Stereospecificity in Coenzyme B12-dependent Diol Dehydratase

2003 ◽  
Vol 278 (25) ◽  
pp. 22717-22725 ◽  
Author(s):  
Naoki Shibata ◽  
Yuka Nakanishi ◽  
Masaki Fukuoka ◽  
Mamoru Yamanishi ◽  
Noritake Yasuoka ◽  
...  
Keyword(s):  
Coenzyme B12 ◽  
Diol Dehydratase

Functions of the D-Ribosyl Moiety and the Lower Axial Ligand of the Nucleotide Loop of Coenzyme B12 in Diol Dehydratase and Ethanolamine Ammonia-lyase Reactions

2002 ◽  
Vol 132 (6) ◽  
pp. 935-943 ◽  
Author(s):  
M. Fukuoka ◽  
S. Yamada ◽  
S. Miyoshi ◽  
K. Yamashita ◽  
M. Yamanishi ◽  
...  
Keyword(s):  
Axial Ligand ◽  
Coenzyme B12 ◽  
Diol Dehydratase
Sign in / Sign up

Export Citation Format

Share Document