Gamma-linolenic acid alters the composition of mitochondrial membrane subfractions, decreases outer mitochondrial membrane binding of hexokinase and alters carnitine palmitoyltransferase I properties in the Walker 256 rat tumour

2002 ◽  
Vol 1583 (1) ◽  
pp. 74-84 ◽  
Author(s):  
Alison Colquhoun
Keyword(s):  
Linolenic Acid ◽  
Mitochondrial Membrane ◽  
Membrane Binding ◽  
Carnitine Palmitoyltransferase ◽  
Outer Mitochondrial Membrane ◽  
Gamma Linolenic Acid ◽  
Carnitine Palmitoyltransferase I ◽  
Walker 256

scholarly journals Rat Liver Carnitine Palmitoyltransferase 1 Forms an Oligomeric Complex within the Outer Mitochondrial Membrane

2007 ◽  
Vol 282 (37) ◽  
pp. 26908-26916 ◽  
Author(s):  
Audrey Faye ◽  
Catherine Esnous ◽  
Nigel T. Price ◽  
Marie Anne Onfray ◽  
Jean Girard ◽  
...  
Keyword(s):  
Rat Liver ◽  
Mitochondrial Membrane ◽  
Carnitine Palmitoyltransferase ◽  
Outer Mitochondrial Membrane ◽  
Oligomeric Complex ◽  
Carnitine Palmitoyltransferase 1

scholarly journals Bax-type Apoptotic Proteins Porate Pure Lipid Bilayers through a Mechanism Sensitive to Intrinsic Monolayer Curvature

2002 ◽  
Vol 277 (51) ◽  
pp. 49360-49365 ◽  
Author(s):  
Gorka Basañez ◽  
Juanita C. Sharpe ◽  
Jennifer Galanis ◽  
Teresa B. Brandt ◽  
J. Marie Hardwick ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Mitochondrial Membrane ◽  
Pore Formation ◽  
Membrane Binding ◽  
Membrane Permeabilization ◽  
Outer Mitochondrial Membrane ◽  
Intrinsic Curvature ◽  
Differential Effects ◽  
Cleavage Fragment ◽  
Induced Changes

During apoptosis, Bax-type proteins permeabilize the outer mitochondrial membrane to release intermembrane apoptogenic factors into the cytosol via a poorly understood mechanism. We have proposed that Bax and ΔN76Bcl-xL(the Bax-like cleavage fragment of Bcl-xL) function by forming pores that are at least partially composed of lipids (lipidic pore formation). Since the membrane monolayer must bend during lipidic pore formation, we here explore the effect of intrinsic membrane monolayer curvature on pore formation. Nonlamellar lipids with positive intrinsic curvature such as lysophospholipids promoted membrane permeabilization, whereas nonlamellar lipids with negative intrinsic curvature such as diacylglycerol and phosphatidylethanolamine inhibited membrane permeabilization. The differential effects of nonlamellar lipids on membrane permeabilization were not correlated with lipid-induced changes in membrane binding or insertion of Bax or ΔN76Bcl-xL. Altogether, these results are consistent with a model whereby Bax-type proteins change the bending propensity of the membrane to form pores comprised at least in part of lipids in a structure of net positive monolayer curvature.

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