scholarly journals The D1/D2 domain of the large-subunit rDNA of the yeast species is unusually polymorphic

2003 ◽  
Vol 4 (3) ◽  
pp. 253-258 ◽  
Author(s):  
M LACHANCE ◽  
H DANIEL ◽  
W MEYER ◽  
G PRASAD ◽  
S GAUTAM ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
D2 Domain ◽  
Large Subunit Rdna

scholarly journals Candida heliconiae sp. nov., Candida picinguabensis sp. nov. and Candida saopaulonensis sp. nov., three ascomycetous yeasts from Heliconia velloziana (Heliconiaceae)

2006 ◽  
Vol 56 (5) ◽  
pp. 1147-1151 ◽  
Author(s):  
Carla C. C. Ruivo ◽  
Marc-André Lachance ◽  
Carlos A. Rosa ◽  
Maurício Bacci ◽  
Fernando C. Pagnocca
Keyword(s):  
Forest Ecosystem ◽  
Yeast Species ◽  
Large Subunit ◽  
Sequence Divergence ◽  
Eastern Brazil ◽  
D2 Domain ◽  
Large Subunit Rdna ◽  
Ascomycetous Yeasts ◽  
Year 2000 ◽  
Type Strains

Strains belonging to three novel yeast species, Candida heliconiae (four isolates), Candida picinguabensis (three isolates) and Candida saopaulonensis (two isolates), were recovered in the year 2000 from water of flower bracts of Heliconia velloziana L. Emigd. (Heliconiaceae) found in a forest ecosystem site in an Atlantic rainforest of south-eastern Brazil. C. picinguabensis and C. saopaulonensis were nearly identical in morphology and physiology, but sequence divergence in the D1/D2 domain of the large-subunit rDNA indicated that they should be regarded as different species. They belong to the Metschnikowiaceae clade. C. heliconiae had affinities to Pichia mexicana and related species, but was genetically isolated from all currently accepted species in that group. The type strains are C. heliconiae UNESP 00-91C1T (=CBS 10000T=NRRL Y-27813T), C. picinguabensis UNESP 00-89T (=CBS 9999T=NRRL Y-27814T) and C. saopaulonensis UNESP 00-99T (=CBS 10001T=NRRL Y-27815T).

scholarly journals Candida bombiphila sp. nov., a new asexual yeast species in the Wickerhamiella clade

2004 ◽  
Vol 54 (5) ◽  
pp. 1857-1859 ◽  
Author(s):  
Michael Brysch-Herzberg ◽  
Marc-André Lachance
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Yeast Strains ◽  
D2 Domain ◽  
Large Subunit Rdna

Two yeast strains were isolated from a bumblebee and bumblebee honey. The strains were almost identical in their D1/D2 domain of the large-subunit rDNA and their physiological abilities. In both respects the strains resembled Wickerhamiella domercqiae. On the basis of these data, it is proposed that the strains represent a novel species with the name Candida bombiphila sp. nov. The type strain is CBS 9712T (=NRRL Y-27640T=MH268T).

scholarly journals Candida floccosa sp. nov., a novel methanol-assimilating yeast species

2006 ◽  
Vol 56 (8) ◽  
pp. 2015-2018 ◽  
Author(s):  
Gábor Péter ◽  
Dénes Dlauchy ◽  
Judit Tornai-Lehoczki
Keyword(s):  
Type Strain ◽  
Quercus Rubra ◽  
Yeast Species ◽  
Large Subunit ◽  
Red Oak ◽  
Sessile Oak ◽  
Yeast Strains ◽  
D2 Domain ◽  
Large Subunit Rdna ◽  
Ascospore Formation

Two methanol-assimilating yeast strains were isolated from a flux of a sessile oak (Quercus petrea) in Hungary and one genetically and phenotypically very similar strain from a flux of a red oak (Quercus rubra) in Canada. The strains exhibited ascomycetous affinity but ascospore formation was not observed. On the basis of the sequence of their D1/D2 domain of the large subunit rDNA, as well as of their physiological characteristics, they represent a novel yeast species of the genus Candida. Therefore Candida floccosa sp. nov. is proposed, with NCAIM Y.01581T (=CBS 10307T=NRRL Y-27951T) as the type strain.

scholarly journals Pichia thermomethanolica sp. nov., a novel thermotolerant, methylotrophic yeast isolated in Thailand

2005 ◽  
Vol 55 (5) ◽  
pp. 2225-2229 ◽  
Author(s):  
Savitree Limtong ◽  
Nantana Srisuk ◽  
Wichien Yongmanitchai ◽  
Hiroya Yurimoto ◽  
Takashi Nakase ◽  
...  
Keyword(s):  
Room Temperature ◽  
Yeast Species ◽  
Large Subunit ◽  
Methylotrophic Yeast ◽  
Good Growth ◽  
Phenotypic Characteristics ◽  
Nucleotide Substitutions ◽  
D2 Domain ◽  
A Cell ◽  
Large Subunit Rdna

Three strains (N002, N069 and PT31T) of a novel thermotolerant methylotrophic yeast species belonging to the genus Pichia were isolated from soil collected in Thailand by three consecutive enrichments in methanol broth at room temperature. They were categorized as thermotolerant strains on the basis of their good growth below 20 °C and up to a high temperature (37 °C). The major characteristics of the three strains included the following and placed them in the genus Pichia: the formation of four helmet-/hat-shaped ascospores in a deliquescent ascus that might be unconjugated or produced by conjugation between a cell and its bud or between independent cells; multilateral budding; the presence of ubiquinone Q-7; negative for Diazonium blue B colour and urease reactions; and the absence of arthrospores and ballistospores. The three strains differed by one to three nucleotide substitutions in the sequences of the D1/D2 domain of the large-subunit rDNA sequence. Phylogenetic analysis revealed that their closest species was Pichia dorogensis, but with 11–13 nucleotide substitutions in 554 nt. The phenotypic characteristics of the three strains were the same. The strains could be distinguished from P. dorogensis by a number of phenotypic characteristics. On the basis of the above findings, these three strains were assigned to a single novel species of Pichia, for which the name Pichia thermomethanolica sp. nov. is proposed. The type strain is PT31T (=BCC 16875T=JCM 12984T=CBS 10098T).

scholarly journals Candida cellulosicola sp. nov., a xylose-utilizing anamorphic yeast from rotten wood

2012 ◽  
Vol 62 (1) ◽  
pp. 242-245 ◽  
Author(s):  
Xinyan Guo ◽  
Hongkun Zhu ◽  
Feng-Yan Bai
Keyword(s):  
Yeast Species ◽  
Southern China ◽  
Large Subunit ◽  
The Novel ◽  
Internal Transcribed Spacer Region ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
Anamorphic Yeast ◽  
Large Subunit Rdna

Two xylose-utilizing yeast strains isolated from rotten wood collected in the rainforest in different mountains of Hainan province, southern China, were studied. Sequence analysis of the large subunit rDNA D1/D2 domain and internal transcribed spacer region revealed that the strains represent a novel anamorphic yeast species, for which the name Candida cellulosicola sp. nov. is proposed; the type strain is HNX16-2T ( = CGMCC 2.3503T = CBS 11952T). Phylogenetically, the novel species was closely related to a xylose-utilizing teleomorphic ascomycetous yeast species Spencermartinsiella europaea in the family Trichomonascaceae, but differed from the latter by 3.0 % mismatches in the D1/D2 domain.

Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 393-397 ◽  
Author(s):  
Stephen A. James ◽  
Enrique Javier Carvajal Barriga ◽  
Patricia Portero Barahona ◽  
Kathryn Cross ◽  
Christopher J. Bond ◽  
...  
Keyword(s):  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Rotten Wood ◽  
D2 Domain ◽  
Lsu Rrna Gene

In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004T) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the Metschnikowia clade and to be most closely related to Candida suratensis, a species recently discovered in a mangrove forest in Thailand. The species name of Candida ecuadorensis sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004T ( = CBS 12653T = NCYC 3782T) designated as the type strain.

Kodamaea kakaduensisandCandida tolerans, two new ascomycetous yeast species from AustralianHibiscusflowers

1999 ◽  
Vol 45 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Marc-André Lachance ◽  
Jane M Bowles ◽  
William T Starmer ◽  
J Stuart F. Barker
Keyword(s):  
Ribosomal Dna ◽  
Yeast Species ◽  
Large Subunit ◽  
New Yeast Species ◽  
Ascomycetous Yeast ◽  
Carbon And Nitrogen ◽  
D2 Domain ◽  
New Yeast ◽  
Type Strains ◽  
Candida Mogii

Two new yeast species were isolated from flowers of Hibiscus species in Eastern and Northern Australia. Kodamaea kakaduensis is heterothallic, haploid, and similar to other Kodamaea species and to Candida restingae. Buds are often produced on short protuberances, and a true mycelium is formed. The new species differs from others by the assimilation of trehalose, melezitose, and xylitol, and is reproductively isolated. The cells of Candida tolerans are small and a pseudomycelium is formed. The carbon and nitrogen assimilation pattern is reminiscent of that of Zygosaccharomyces rouxii but the two are not closely related. Sequences of the D1/D2 domain of large subunit ribosomal DNA confirm the membership of K. kakaduensis in the genus Kodamaea and indicate that C. tolerans belongs to the Clavispora-Metschnikowia clade, with a moderate relatedness to Candida mogii. The type strains are: K. kakaduensis, UWO(PS)98-119.2 (h+, holotype, CBS 8611) and UWO(PS)98-117.1 (h-, isotype, CBS 8612); and C. tolerans, UWO(PS)98-115.5 (CBS 8613).Key words: Kodamaea, Candida, new yeast species, ribosomal DNA, whole-cell PCR.

scholarly journals Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest

2011 ◽  
Vol 61 (2) ◽  
pp. 454-461 ◽  
Author(s):  
Somjit Am-In ◽  
Savitree Limtong ◽  
Wichien Yongmanitchai ◽  
Sasitorn Jindamorakot
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Type Strain ◽  
Mangrove Forest ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Rrna Gene ◽  
D2 Domain ◽  
Large Subunit Rrna

Five strains (RV5T, RV140, R31T, RS17 and RS28T) representing three novel anamorphic ascomycetous yeast species were isolated by membrane filtration from estuarine waters collected from a mangrove forest in Laem Son National Park, Ranong Province, Thailand, on different occasions. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and the internal transcribed spacer region and phylogenetic analysis, three strains were found to represent two novel Candida species. Two strains (RV5T and RV140) represented a single novel species, for which the name Candida laemsonensis sp. nov. is proposed. The type strain is RV5T (=BCC 35154T =NBRC 105873T =CBS 11419T). Strain R31T was assigned to a novel species that was named Candida andamanensis sp. nov. (type strain R31T =BCC 25965T =NBRC 103862T =CBS 10859T). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 domain of the large-subunit rRNA gene and phylogenetic analysis, strains RS17 and RS28T represented another novel species of Candida, for which the name Candida ranongensis sp. nov. is proposed. The type strain is RS28T (=BCC 25964T =NBRC 103861T =CBS 10861T).

scholarly journals Clavispora santaluciae f.a., sp. nov., a novel ascomycetous yeast species isolated from grapes

2020 ◽  
Vol 70 (12) ◽  
pp. 6307-6312
Author(s):  
João Drumonde-Neves ◽  
Neža Čadež ◽  
Yazmid Reyes-Domínguez ◽  
Andreas Gallmetzer ◽  
Dorit Schuller# ◽  
...  
Keyword(s):  
Yeast Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Drosophila Suzukii ◽  
Nucleotide Substitutions ◽  
Ascomycetous Yeast ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
Additional Strain

During a study of yeast diversity in Azorean vineyards, four strains were isolated which were found to represent a novel yeast species based on the sequences of the internal transcribed spacer (ITS) region (ITS1-5.8S–ITS2) and of the D1/D2 domain of the large subunit (LSU) rRNA gene, together with their physiological characteristics. An additional strain isolated from Drosophila suzukii in Italy had identical D1/D2 sequences and very similar ITS regions (five nucleotide substitutions) to the Azorean strains. Phylogenetic analysis using sequences of the ITS region and D1/D2 domain showed that the five strains are closely related to Clavispora lusitaniae, although with 56 nucleotide differences in the D2 domain. Intraspecies variation revealed between two and five nucleotide differences, considering the five strains of Clavispora santaluciae. Some phenotypic discrepancies support the separation of the new species from their closely related ones, such as the inability to grow at temperatures above 35 °C, to produce acetic acid and the capacity to assimilate starch. Neither conjugations nor ascospore formation were observed in any of the strains. The name Clavispora santaluciae f.a., sp. nov., is proposed to accommodate the above noted five strains (holotype, CBS 16465T; MycoBank no., MB 835794).

scholarly journals Description of four Apiotrichum and two Cutaneotrichosporon species isolated from guano samples from bat-inhabited caves in Japan

2020 ◽  
Vol 70 (8) ◽  
pp. 4458-4469
Author(s):  
Masako Takashima ◽  
Sanae Kurakado ◽  
Otomi Cho ◽  
Ken Kikuchi ◽  
Junta Sugiyama ◽  
...  
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Large Subunit ◽  
Biochemical Properties ◽  
Rrna Genes ◽  
New Yeast Species ◽  
Rrna Gene ◽  
D2 Domain ◽  
Lsu Rrna ◽  
New Yeast

Four new yeast species belonging to the genus Apiotrichum and two new yeast species belonging to Cutaneotrichosporon are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as Trichosporon species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe Apiotrichum akiyoshidainum (holotype JCM 12595T), Apiotrichum chiropterorum (JCM 12594T), Apiotrichum coprophilum (JCM 12596T), Apiotrichum otae (JCM 12593T), Cutaneotrichosporon cavernicola (JCM 12590T) and Cutaneotrichosporon middelhovenii (JCM 12592T) as new species. C. cavernicola showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.

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