Evaluation of Antinociceptive Activity of Methanol Extract from Cleome rutidosperma in Mice

Background: Carissa carandas Linn. (Bengali name- Karamya, Karancha, Karamcha, English name-Karaunda) commonly known as Karanda is a widly used medicinal plants belongs to apocynaceae family. The various plant parts of C. carandas have been used for ethnomedicine in the treatment of human diseases, such as diarrhea, stomachic, anorexia, intermittent fever, mouth ulcer and sore throat, syphilitic pain, burning sensation, scabies and epilepsy. The current studies were aimed to investigate the phytochemical constituents, antinociceptive, anthelmintic and cytotoxic activities of the methanol extract of leaves of C. carandas.Methods: The antinociceptive property of the methanol leaves extract of C. carandas was evaluated in swiss albino mice by using acetic acid-induced writhing test and anthelmintic activity of the fresh leaves juice was performed by observing the time of paralysis and the time of death of earth worms Pheretima posthuma. On the other hand, the methanol extract of leaves of C. carandas was screened for cytotoxic activity by brine shrimp lethality bioassay. Results: Phytochemical screening showed that the methanol leaves extract contained alkaloids, steroids, flavanoids, tannins, saponins and reducing sugar. The antinociceptive activity test revealed that the extract showed significant antinociceptive activity and the fresh juice of the leaves of C. carandas showed potent anthelmintic activity. In cytotoxicity studies, the methanol leaves extract displayed moderate cytotoxic activity when compared with standard drug, vincristine sulphate.Conclusions: In the current studies, these observations also support the use of this plant for medicinal purposes and encourage further investigations for more fruitful results.

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Antinociceptive and Anthelmintic Activities of Leaves of Leea aequata

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v17i2.39183 ◽

2018 ◽

Vol 17 (2) ◽

pp. 251-255 ◽

Cited By ~ 1

Author(s):

Sushanta Halder ◽

Nazmus Saqueeb ◽

Nazmul Qais

Keyword(s):

Crude Extract ◽

Methanol Extract ◽

Diclofenac Sodium ◽

Anthelmintic Activity ◽

Radiant Heat ◽

Antinociceptive Activity ◽

Dependent Manner ◽

Standard Drug ◽

Dose Dependent ◽

Crude Methanol Extract

The objective of the study was to evaluate the antinociceptive and anthelmintic activities of the crude methanol extract of leaves of Leea aequata and its fractions. The crude extract and its fractions at 200- and 400-mg/kg bw were subjected to assay for their antinociceptive activity using acetic acid induced writhing and radiant heat tail flicking methods. The ethyl acetate soluble fraction at 400 mg/kg bw induced 40.97% inhibition of writhing in mice while the carbon tetrachloride and chloroform soluble fractions of crude extract at the same dose displayed activity with 40.28% inhibition of writhing as compared to standard diclofenac sodium. The crude extract elongated the reaction time by 57.04% after 30 minute of administration in radiant heat tail flicking method, which suggested the central antinociceptive activity as compared to morphine. The methanol extract of the leaves of L. aequata exhibited profound anthelmintic activity in a dose dependent manner with shortest time of paralysis and death at 100 mg/ml concentration. It caused paralysis of the earthworm Pheretima posthuma at 9.44 min and death at 12.9 min when compared to the standard drug albendazole, which at 10 mg/ml concentration revealed the same at 8.21 minutes and 11.18 minutes, respectively. Dhaka Univ. J. Pharm. Sci. 17(2): 251-255, 2018 (December)

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Antinociceptive activity of methanol extract of fruits of Capparis ovata in mice

Journal of Ethnopharmacology ◽

10.1016/j.jep.2010.05.060 ◽

2010 ◽

Vol 131 (1) ◽

pp. 28-32 ◽

Cited By ~ 28

Author(s):

Rana Arslan ◽

Nurcan Bektas ◽

Yusuf Ozturk

Keyword(s):

Methanol Extract ◽

Antinociceptive Activity

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Antinociceptive Activity of Methanol Extract ofMuntingia calaburaLeaves and the Mechanisms of Action Involved

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/890361 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 18

Author(s):

M. H. Mohd. Sani ◽

Z. A. Zakaria ◽

T. Balan ◽

L. K. Teh ◽

M. Z. Salleh

Keyword(s):

Methanol Extract ◽

Methyl Esters ◽

Cyclic Guanosine Monophosphate ◽

Guanosine Monophosphate ◽

Antinociceptive Activity ◽

Opioid Antagonist ◽

Hot Plate Test ◽

No Donor ◽

Cgmp Pathway

Muntingia calaburaL. (family Elaeocarpaceae) has been traditionally used to relieve various pain-related ailments. The present study aimed to determine the antinociceptive activity of methanol extract ofM. calaburaleaves (MEMC) and to elucidate the possible mechanism of antinociception involved. Thein vivochemicals (acetic acid-induced abdominal constriction and formalin-, capsaicin-, glutamate-, serotonin-induced paw licking test) and thermal (hot plate test) models of nociception were used to evaluate the extract antinociceptive activity. The extract (100, 250, and 500 mg/kg) was administered orally 60 min prior to subjection to the respective test. The results obtained demonstrated that MEMC produced significant (P<0.05) antinociceptive response in all the chemical- and thermal-induced nociception models, which was reversed after pretreatment with 5 mg/kg naloxone, a non-selective opioid antagonist. Furthermore, pretreatment with L-arginine (a nitric oxide (NO) donor), NG-nitro-L-arginine methyl esters (L-NAME; an inhibitor of NO synthase (NOS)), methylene blue (MB; an inhibitor of cyclic-guanosine monophosphate (cGMP) pathway), or their combination also caused significant (P<0.05) change in the intensity of the MEMC antinociception. In conclusion, the MEMC antinociceptive activity involves activation of the peripheral and central mechanisms, and modulation via, partly, the opioid receptors and NO/cGMP pathway.

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