Efikasi Herbisida Campuran Tienkarbazon Metil 68 g/l dan Tembotrion 345 g/l Terhadap Gulma Berdaun Lebar dan Gulma Golongan Rumput Pada Budidaya Tanaman Jagung (Zea mays L.).

Kompetisi nutrisi akibat kehadiran gulma di area pertanaman jagung dapat menyebabkan kehilangan hasil yang signifikan pada tanaman jagung. Penggunaan campuran herbisida merupakan salah satu upaya dalam mengendalikan gulma serta menghindari pembentukan gulma yang resisten. Tujuan penelitian ini adalah mengetahui pengaruh herbisida Tienkarbazon Metil 68 g/l dan Tembotrion 345 g/l dalam mengendalikan gulma berdaun lebar dan gulma rumput pada pertanaman jagung. Penelitian dilakukan pada bulan Oktober 2020 hingga Januari 2021 di Lahan Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Kampus Jatinangor, Kabupaten Sumedang. Tempat penelitian terletak pada ketinggian ± 752 mdpl. Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 ulangan. Perlakuan percobaan terdiri dari herbisida Tienkarbazon Metil 68 g/l dan Tembotrion 345 g/l dosis 150, 225, 300, 375, 450 ml/ha, penyiangan manual, dan tanpa perlakuan. Hasil penelitian yang dilakukan menunjukkan bahwa herbisida berbahan aktif Tienkarbazon Metil 68 g/l dan Tembotrion 345 g/l dimulai dari dosis 150 hingga 450 ml/ha efektif mengendalikan gulma berdaun lebar (Alternanthera sesilis, Cleome rutidosperma, Portulaca oleracea, Eleusina indica), gulma rumput (Digitaria ciliaris, Paspalum conjugatum, dan Amaranthus sp), dan gulma total hingga 6 minggu setelah aplikasi tanpa menimbulkan efek keracunan pada pertanaman jagung. Kata Kunci: Gulma, Jagung, Tienkarbazon metil 68 g/l + Tembotrion 345 g/l.

Cleome rutidosperma (fringed spiderflower).

C. rutidosperma is a common herb that grows as a weed in disturbed and ruderal habitats, principally in areas with humid and hot environmental conditions. It is often found as a weed of disturbed ground, roadsides, gardens, crops and abandoned lands, and has also been found growing as an epiphyte on trees, stone walls and cliff faces. This species is included in the Global Compendium of Weeds (Randall, 2012) where it is considered to have moderate economic impacts in a wide range of crops, due to its scrambling habit that smothers and stunts young crop plants. C. rutidosperma has been listed as invasive in China, Malaysia, India, Thailand, Vietnam, Australia, and the Domican Republic (Waterhouse and Mitchell, 1998; Kairo et al., 2003; Flora of China Editorial Committee, 2014, USDA-ARS, 2014). This species has had considerable environmental impacts in South East Asia and Australia. C. rutidosperma also has the potential to be moderately problematic in intensive cropping areas, greenhouses and nurseries.

Bioherbicidal Properties of Parthenium hysterophorus, Cleome rutidosperma and Borreria alata Extracts on Selected Crop and Weed Species

Natural product-based herbicides could be the effective alternatives to synthetic chemical herbicides for eco-friendly weed management. This research, therefore, was conducted to identify the phytotoxic properties of Parthenium hysterophorus L., Cleome rutidosperma DC. and Borreria alata (Aubl.) DC. with a view to introducing them as a tool for natural herbicide development. The methanol extracts of these plants were examined on the germination and growth of Zea mays L., Oryza sativa L., Abelmoschus esculentus (L.) Moench and Amaranthus gangeticus L., Oryza sativa f. Spontanea Roshev. (Weedy rice), Echinochloa colona (L.) Link., Euphorbia hirta L., and Ageratum conyzoides L. under laboratory and glasshouse conditions. A complete randomized design (CRD) with five replications and randomized complete block design (RCBD) with four replications were laid out for laboratory and glasshouse experiments, respectively. In the laboratory experiment, three plant extracts of 0, 6.25, 12.5, 50, and 100 g L−1 were tested on survival rate, hypocotyl, and radicle length of eight test plant species. No seed germination of A. conzyoides, E. hirta, and A. gangeticus were recorded when P. hysterophorus extract was applied at 50 g L−1. C. rutidosperma had the same effect on those plants at 100 g L−1. In the glasshouse, similar extracts and concentrations used in the laboratory experiments were sprayed on at the 2–3 leaf stage for grasses and 4–6 for the broadleaf species. Tested plants were less sensitive to C. rutidosperma and B. alata compared to P. hysterophorus extract. Among the weeds and crops, A. conyzoides, E. hirta, A. esculentus and A. gangeticus were mostly inhibited by P. hysterophorus extract at 100 g L−1. Based on these results, P. hysterophorus was the most phytotoxic among the tested plant extracts and could be used for developing a new natural herbicide for green agriculture.

New distributional records of Cleome chelidonii L.f. and Cleome rutidosperma DC. (Cleomaceae) in Madura Island

Calcareous soil and dry climate are characteristic of Madura Island, located on the east coast of Java, Indonesia. The group of flowering plants that adapted to these conditions is the genus Cleome L. (Cleomaceae). In 1963, Backer and Bakhuizen van den Brink Jr. only listed three species of Cleome from Madura, i.e., C. aspera J.König ex DC., C. gynandra L., and C. viscosa L. Since then, the updated data on the genus Cleome of Madura is not provided yet. Therefore, this study aimed to provide updated information on the genus in Madura island. The botanical exploration was conducted at Bangkalan, Sampang, Pamekasan, and Sumenep in December 2019 to January 2020. The two additional species, namely C. chelidonii L.f. and C. rutidosperma DC., were reported for the first time in Madura. Both species were considered as the newly distributional record for the island. Cleome chelidonii was found in Gapura Tengah (Sumenep Regency), and C. rutidosperma was collected from Kamal (Bangkalan Regency). The botanical information on both species is presented, including the updated key to the Cleome of Madura Island. This study provides updated information on genus Cleome in Madura and reporting the existence of Cleome chelidonii and Clome rutidosperma from the island. The presence of both species was considered as a newly distributional record.

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

Background: Medicinal plants are a source of phytochemicals and they are used for the treatment of several oxidative stress-related or other diseases for their effectiveness, low toxicity and easy availability. Five traditionally used and less characterized herbaceous weeds of West Bengal, India, namely, Heliotropium indicum, Tridax procumbens, Cleome rutidosperma, Commelina benghalensis and Euphorbia hirta, were investigated for the current research study. Methods: Aqueous and 70% ethanolic extracts of the leaves were analyzed for estimation of essential phytochemicals and to evaluate their in vitro antioxidant status, medicinal properties and cytotoxic effects. To the best of our knowledge, several assays and comparative evaluations using these herbs are reported for the first time. For quantitative study, UV-vis spectrophotometry and high-performance liquid chromatography with diode array detector HPLC-DAD techniques were used. Antibacterial properties were investigated using the Kirby-Bauer disc diffusion method. For in vitro anti-lithiatic study, a titration method was used. The cell viability assay was done using peripheral blood mononuclear cells. Results: The aqueous extract exhibits higher content of polyphenols, flavonoids, tannins and inhibition percentage values for free radical scavenging assays, whereas the 70% ethanolic extract exhibits higher content of alkaloids and cardiac glycosides. HPLC-DAD analysis of 70% ethanolic extracts led us to identify 10 predominant phenolic constituents. Euphorbia hirta extracts showed minimum cytotoxicity (cell death ~2.5% and 4% in water and 70% ethanolic extract, respectively), whereas Cleome rutidosperma and Tridax procumbens’ 70% ethanolic extracts showed higher cell death (~13% and 28%, respectively), compared with the control (cell death ~10-12%). Conclusions: The study concluded that of all the medicinal weeds selected for the current study, Euphorbia hirta possesses the highest amount of bioactive compounds and hence exhibits the highest in vitro antioxidant activity and promising in vitro medicinal properties.

Antioxidant Activities, Nutritional Composition and Antinutritional Properties of Two Leafy Vegetables (Cleome rutidosperma and Cassia tora) Consumed in Adamawa State, Nigeria

Antioxidants are agents which scavenge free radicals and prevent cellular damage cause by them. They reduce the damage due to free radicals by neutralizing them before they can attack the cells. The aim of this research was to evaluate the antioxidant, nutritional and antinutritional properties of two leafy vegetables, Cleome rutidosperma and Cassia tora consumed in Adamawa State, Nigeria. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) models were used to determine antioxidant activities of extracts. Nutritional composition and antinutritional properties were determined using standard procedures. There was a significant (p=0.05) decrease in the concentration of DPPH radical due to the scavenging activity of ethanol leaf extract of Cleome rutidosperma compared to control/standard. In FRAP also, C. rutidosperma ethanolic extract exhibited higher ferric reducing power than C. tora. Proximate analysis revealed the nutrients for C. rutidosperma and C. tora as; crude protein (31.06±0.00% and 26.24±0.00%), fat (7.13±0.00% and 6.93±0.10%), ash (15.17±0.61% and 11.60±0.00%), crude fiber (11.06±0.01 and 13.19±0.10%), moisture (9.99±0.00% and 9.12±0.10%), NFE (25.60±0.10% and 32.94±0.10%) respectively. Antinutritionals in ethanolic extracts of C. rutidosperma and C. tora were; oxalates, saponins, tannins, cyanogenic glycosides, phytate and alkaloids. Both vegetables revealed good percentages of proteins which can be used to compliment other sources of protein. All antinutrients of extracts were within tolerable levels.

Reversal of Diabetic Complications in Andrology Parameters of Alloxan-Induced Diabetic Male Wistar Rats Treated with Cleome rutidosperma Leaves

Male infertility is one of the complications associated with diabetes mellitus. This study reported the effect of managing experimentally-induced diabetes in male Wistar rats with Cleome rutidosperma leaf extract at doses of 100 mg.kg−1, 200 mg.kg−1 or 400 mg.kg−1 for 14 days. Further deterioration or amelioration of reproductive derangement was assessed by changes in sperm morphology, sperm characteristics, and testicular histo-pathology. Andrology profile of diabetic untreated rats showed significant (P < 0.05) derangements compared to normoglycaemic rats. The profile of diabetic rats treated with the extract of C. rutidosperma, especially at the 200 mg.kg−1 dose showed a significant decrease in abnormal sperm cells, increases in sperm motility, live/ dead ratio and count compared to the untreated diabetic rats. Histopathology showed spermatogenic arrest and degeneration of Sertoli cells in the diabetic untreated rats, but was restored in the C. rutidosperma treated rats. This study showed evidence of infertility associated with diabetes and the ameliorative ability of C. rutidosperma on infertility demonstrated by improved andrological parameters. Traditional management of diabetes with C. rutidosperma leaves should therefore be encouraged in both humans and other animals, especially males with reproductive intentions.