Light scatter applications

A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the walls of the bright vessels easily detectable from the dark background.

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Effects of light scatter, defocusing, mean luminosity, contrast, and central scotoma on the PVER amplitude-check size function curve

Acta Ophthalmologica ◽

10.1111/j.1755-3768.1993.tb04649.x ◽

2009 ◽

Vol 71 (5) ◽

pp. 606-615 ◽

Cited By ~ 2

Author(s):

Osamu Katsumi ◽

Hitomi Tetsuka ◽

Soichi Tetsuka ◽

Young Hoon-Ohn ◽

Tatsuo Hirose

Keyword(s):

Light Scatter ◽

Central Scotoma ◽

Check Size ◽

Size Function

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Islet cell analysis and purification by light scatter and autofluorescence

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(82)91523-6 ◽

1982 ◽

Vol 107 (2) ◽

pp. 525-532 ◽

Cited By ~ 57

Author(s):

Marnix Van de Winkel ◽

Eddie Maes ◽

Daniël Pipeleers

Keyword(s):

Islet Cell ◽

Light Scatter ◽

Cell Analysis

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Small-angle HeNe laser light scatter and the compressive modulus of articular cartilage

Journal of Orthopaedic Research® ◽

10.1002/jor.1100150317 ◽

1997 ◽

Vol 15 (3) ◽

pp. 437-441 ◽

Cited By ~ 8

Author(s):

Ian S. Kovach ◽

Kyriacos A. Athanasiou

Keyword(s):

Articular Cartilage ◽

Small Angle ◽

Laser Light ◽

Compressive Modulus ◽

Light Scatter ◽

Hene Laser

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Vortex evolution in a round jet

Journal of Fluid Mechanics ◽

10.1017/s0022112068000248 ◽

1968 ◽

Vol 31 (3) ◽

pp. 435-448 ◽

Cited By ~ 160

Author(s):

H. A. Becker ◽

T. A. Massaro

Keyword(s):

Maximum Growth ◽

Maximum Growth Rate ◽

Ring Vortex ◽

Acoustic Excitation ◽

Light Scatter ◽

Turbulent Eddies ◽

Cylindrical Vortex ◽

Highly Sensitive ◽

Pure Tones ◽

Axisymmetrical Jet

A study has been made of the varicose instability of an axisymmetrical jet with a velocity distribution radially uniform at the nozzle mouth except for a laminar boundary layer at the wall. The evolutionary phenomena of instability, such as the rolling up of the cylindrical vortex layer into ring vortices, the coalescence of ring vortex pairs, and the eventual disintegration into turbulent eddies, have been investigated as a function of the Reynolds number using smoke photography, stroboscopic observation, and the light-scatter technique.Emphasis has been placed on the wavelength with maximum growth rate. The jet is highly sensitive to sound and the effects of several types of acoustic excitation, including pure tones, have been determined.

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