Parental chromosome testing

2010 ◽  
pp. 91-102 ◽  
Author(s):  
M. Goddijn ◽  
Nico J. Leschot
Keyword(s):  
Parental Chromosome

The Analysis of Malignancy by Cell Fusion

1971 ◽  
Vol 8 (3) ◽  
pp. 673-680
Author(s):  
U. BREGULA ◽  
G. KLEIN ◽  
H. HARRIS
Keyword(s):  
Cell Fusion ◽  
Chromosomal Analysis ◽  
Cell Populations ◽  
Hybrid Cells ◽  
Parental Chromosome ◽  
Chromosomal Constitution ◽  
Diploid Fibroblasts ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

When Ehrlich ascites cells were fused with diploid fibroblasts, isolated directly from the animal, the resulting hybrid cells regularly produced progressive tumours. However, an analysis of a range of clonal populations of these hybrid cells, each derived from a separate primary fusion, revealed that the chromosomal constitution of these cells was highly unstable; all cell populations were found to have already undergone substantial chromosome losses by the time enough cells were available to permit chromosomal analysis. Thus, although these hybrid cells were highly tumorigenic, the tumours arising from them were not composed of cells with complete parental chromosome sets, but of cells from which some chromosomes had been eliminated.

Partial F trisomy associated with familial F/13 translocation detected and identified by parental chromosome studies

1971 ◽  
Vol 78 (4) ◽  
pp. 664-672 ◽  
Author(s):  
Robert E. Carrel ◽  
Robert S. Sparkes ◽  
Stanley W. Wright
Keyword(s):  
Parental Chromosome

scholarly journals Formamide-Free Genomic in situ Hybridization Allows Unambiguous Discrimination of Highly Similar Parental Genomes in Diploid Hybrids and Allopolyploids

2015 ◽  
Vol 146 (4) ◽  
pp. 325-331 ◽  
Author(s):  
Tae-Soo Jang ◽  
Hanna Weiss-Schneeweiss
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Dna ◽  
Species Complex ◽  
Genomic In Situ Hybridization ◽  
Genomic Evolution ◽  
Parental Chromosome ◽  
Unambiguous Discrimination ◽  
Modified Method ◽  
Prospero Autumnale

Polyploidy and hybridization play an important role in plant diversification and speciation. The application of genomic in situ hybridization (GISH) allows the identification of parental genomes in hybrids, thus elucidating their origins and allowing for analysis of their genomic evolution. The performance of GISH depends on the similarity of the parental genomes and on the age of hybrids. Here, we present the formamide-free GISH (ff-GISH) protocol applied to diploid and polyploid hybrids of monocots (Prospero, Hyacinthaceae) and dicots (Melampodium, Asteraceae) differing in similarity of the parental genomes and in chromosome and genome sizes. The efficiency of the new protocol is compared to the standard GISH protocol. As a result, ff-GISH allowed efficient labeling and discrimination of the parental chromosome sets in diploid and allopolyploid hybrids in Prospero autumnale species complex. In contrast, the standard GISH protocol failed to differentiate the parental genomes due to high levels of similar repetitive DNA. Likewise, an unambiguous identification of parental genomes in allotetraploid Melampodium nayaritense (Asteraceae) was possible after ff-GISH, whereas the standard GISH hybridization performance was suboptimal. The modified method is simple and non-toxic and allows the discrimination of very similar parental genomes in hybrids. This method lends itself to modifications and improvements and can also be used for FISH.

The Analysis of Malignancy by Cell Fusion

1974 ◽  
Vol 16 (1) ◽  
pp. 189-198
Author(s):  
F. WIENER ◽  
G. KLEIN ◽  
H. HARRIS
Keyword(s):  
Cell Fusion ◽  
Tumour Cells ◽  
Complementation Analysis ◽  
Malignant Cells ◽  
Hybrid Cells ◽  
Malignant Phenotype ◽  
Recessive Character ◽  
Parental Chromosome ◽  
Wide Range

Previous studies with a variety of transplantable mouse tumours showed that in hybrids between malignant and non-malignant cells, malignancy behaved as a recessive character: the hybrid cells, so long as they retained something close to the complete parental chromosome sets, had little or no ability to grow progressively in vivo. In the experiments we now describe the heritable lesions determining the malignant phenotype were further explored by complementation analysis in which the various tumour cells were fused with each other. Forty-two clonal populations derived from twelve crosses between different kinds of tumour cells were examined. Only one cross generated hybrid cells with reduced tumorigenicity: in all other cases the hybrid cells formed were highly malignant. It thus appears that, in a wide range of different tumours, the lesions determining the malignant phenotype, although recessive, fail to complement each other.

AreABLandBCRImprinted?

1996 ◽  
Vol 45 (1-2) ◽  
pp. 233-237
Author(s):  
M. Fink ◽  
A. Argyriou-Tirita ◽  
O.A. Haas
Keyword(s):  
Genomic Imprinting ◽  
Maternal Origin ◽  
Reversible Process ◽  
Parental Chromosome ◽  
Specific Modification ◽  
Clinical Observations ◽  
Functional Differences ◽  
Diploid Cells ◽  
Sporadic Tumors ◽  
Chromosome Regions

An increasing number of clinical observations and genetic experiments have shown that some parts of the genome behave differently depending on whether they are of paternal or maternal origin. This phenomenon is known as “genomic imprinting” and has been defined as “a reversible process whereby a gamete-specific modification in the parental generation can sometimes lead to functional differences between maternal and paternal genomes in diploid cells of the offspring” [1]. The accumulating evidence for its important role in cancer predisposition syndromes as well as for the pathogenesis of certain types of sporadic tumors prompted us to investigate whether imprinting may also be instrumental in selecting particular parental chromosome regions involved in balanced rearrangements, such as the leukemia-specific translocation t(9;22) [2]. Several reports have analyzed the expression, the methylation and the replication patterns of the two genes, ABL and BCR, on chromosomes 9 and 22, respectively, which are affected by this translocation [3-6]. Although not directly comparable, the results of these studies seem to invalidate our cytogenetic observations. We therefore review this controversial issue and provide some possible explanations for the contradictory results.

scholarly journals Parental Chromosome-specific Chromatin Conformation in the ImprintedU2af1-rs1Gene in the Mouse

1997 ◽  
Vol 272 (33) ◽  
pp. 20893-20900 ◽  
Author(s):  
Robert Feil ◽  
Maria D. Boyano ◽  
Nicholas D. Allen ◽  
Gavin Kelsey
Keyword(s):  
Chromatin Conformation ◽  
Parental Chromosome
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