PLANT GROWTH-PROMOTING BACTERIAL ENDOPHYTES FROM SUGARCANE AND THEIR POTENTIAL IN PROMOTING GROWTH OF THE HOST UNDER FIELD CONDITIONS

2012 ◽  
Vol 49 (1) ◽  
pp. 43-52 ◽  
Author(s):  
HEMLATA CHAUHAN ◽  
D. J. BAGYARAJ ◽  
ANITA SHARMA
Keyword(s):  
Plant Growth ◽  
Siderophore Production ◽  
Red Rot ◽  
Research Centre ◽  
Cane Yield ◽  
Colletotrichum Falcatum ◽  
Bacterial Endophytes ◽  
Red Rot Disease ◽  
Plant Growth Promoting ◽  
Growth Promoting

SUMMARYTen endophytic bacteria were isolated from different sugarcane varieties growing in the Crop Research Centre, Pantnagar on nitrogen-free medium. Plant growth-promoting potential of the isolates was reported in terms of indole acetic acid (IAA) production, phosphorus solubilization, siderophore production and antagonistic action against the pathogenColletotrichum falcatum, which causes red rot disease in sugarcanein vitro. All the isolates were able to produce IAA (4.8–9 μg ml−1); three isolates (H3, H5 and H14) solubilized insoluble phosphorus on Pikovaskaya's agar; two isolates (H10 and H14) showed siderophore production on Chrome-azurol S (CAS) agar and antagonism againstC. falcatumwas exhibited by two isolates (H14 and H15) in a dual plate assay. 16 S rRNA sequencing identified isolates H3 and H12 asPseudomonasspp., and H8, H14 and H15 asBacillusspp. A field experiment on sugarcane was conducted with five plant growth-promoting bacterial endophytesPseudomonasspp. (H3 and H12) andBacillusspp. (H8, H14 and H15) along with standard strains ofGluconacetobacterandAzospirillumspp. Plant height, chlorophyll content, total nitrogen and cane length were significantly higher in almost all inoculated plants compared with the uninoculated control. An increase of 40% in cane yield over the control was obtained after inoculation with isolate H15 (Bacillusspp.). This was statistically on par with the standard endophyteGluconacetobacter diazotrophicus, which resulted in 42% increased cane yield. Identification of new diazotrophs and their promising results towards improving plant growth in the field suggest their use as inoculants in future.

Isolation, characterization, and effect of fluorescent pseudomonads on micropropagated sugarcane

2009 ◽  
Vol 55 (8) ◽  
pp. 1007-1011 ◽  
Author(s):  
Samina Mehnaz ◽  
Brian Weselowski ◽  
Faheem Aftab ◽  
Sadaf Zahid ◽  
George Lazarovits ◽  
...  
Keyword(s):  
Nifh Gene ◽  
Fluorescent Pseudomonads ◽  
Red Rot ◽  
Colletotrichum Falcatum ◽  
Rrna Gene ◽  
Red Rot Disease ◽  
Shoot Height ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rot Disease

In this study, we report on the isolation, identification, and characterization of seven fluorescent pseudomonads isolated from the roots, shoots, and rhizosphere soil of sugarcane and their impacts on the growth of sugarcane plantlets. 16S rRNA gene sequence of five isolates showed close homology with Pseudomonas putida , one with Pseudomonas graminis , and one with Pseudomonas fluorescens . Physiological and biochemical characterizations were determined using API50CH and QTS24 identification kits. The isolates were also subjected to tests for various known growth promoting properties including production of indole acetic acid, the ability to fix nitrogen via the presence of the nifH gene, and ability to solubilize phosphate. Biological control potential was determined from agar diffusion assays of HCN production and production of antifungal compounds against local isolates of Colletotrichum falcatum (that induces red-rot disease of sugarcane). Direct plant growth promoting effects were tested on sugarcane plantlets in tissue culture under gnotobiotic conditions. All seven isolates provided significant increases in fresh and dry masses but only five strains increased shoot height.

PLANT GROWTH PROMOTING BACTERIAL ENDOPHYTES ISOLATED FROM POLISH HERBAL PLANTS

2018 ◽  
Vol 17 (5) ◽  
pp. 101-110 ◽  
Author(s):  
Agata Goryluk-Salmonowicz ◽  
Aleksandra Orzeszko-Rywka ◽  
Monika Piórek ◽  
Hanna Rekosz-Burlaga ◽  
Adrianna Otłowska ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacterial Endophytes ◽  
Plant Growth Promoting ◽  
Herbal Plants ◽  
Growth Promoting

scholarly journals Diesel degrading bacterial endophytes with plant growth promoting potential isolated from a petroleum storage facility

3 Biotech ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aneela Iqbal ◽  
Muhammad Arshad ◽  
Raghupathy Karthikeyan ◽  
Terry J. Gentry ◽  
Jamshaid Rashid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Storage Facility ◽  
Bacterial Endophytes ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Petroleum Storage

scholarly journals Selection of the Root Endophyte Pseudomonas brassicacearum CDVBN10 as Plant Growth Promoter for Brassica napus L. Crops

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1788
Author(s):  
Alejandro Jiménez-Gómez ◽  
Zaki Saati-Santamaría ◽  
Martin Kostovcik ◽  
Raúl Rivas ◽  
Encarna Velázquez ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Dry Weight ◽  
Bacterial Endophytes ◽  
Chemical Fertilizers ◽  
Brassica Napus L ◽  
Bacterial Microbiome ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Pseudomonas Brassicacearum

Rapeseed (Brassica napus L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative effects on human health and the environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers, but efficient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial endophytes, protected from the rhizospheric competitors and extreme environmental conditions, could overcome those problems and successfully promote the crops under field conditions. Here, we present a screening process among rapeseed bacterial endophytes to search for an efficient bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on in vitro, in planta, and in silico tests, we selected the strain Pseudomonas brassicacearum CDVBN10 as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial microbiome; considering that the root microbiome plays an important role in plant fitness and development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis as a positive result. Thus, Pseudomonas brassicacearum CDVBN10 seems to be a good biofertilizer to improve canola crops with no addition of chemical fertilizers; this the first study in which a plant growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves this crop’s yields in field conditions.

Siderophore production by plant growth‐promoting pseudomonas SPP.

1988 ◽  
Vol 11 (6-11) ◽  
pp. 925-933 ◽  
Author(s):  
Peter A.H.M. Bakker ◽  
Peter J. Weisbeek ◽  
Bob Schippers
Keyword(s):  
Plant Growth ◽  
Siderophore Production ◽  
Pseudomonas Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting
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