Management of biobanking for medical genetics research

Biobanking is one of the most important elements of the modern infrastructure for biomedical research. Organization of a biobank on the basis of the N. P. Bochkov Medical Genetics Research Center provides a centralized infrastructure for preparing biomaterial for research. Biobank has the format of a research equipment sharing center and works with two types of unique biomaterials from patients with genetic diseases: blood/blood components and vital cells of various tissue origin. The storage facility of the Biobank is equipped with low-temperature (-80° C) and cryostorage (-196° C) systems. Identification and search of samples is carried out using a bar-coding system and is implemented through the information interface of the biobank, which is integrated into the general database of patients at the Medical Genetics Research Center. Information on biomaterial samples is presented in periodically updated catalogs on the page of equipment sharing center “Biobank”. Biobank collection is available to internal and external users.

Innovative Low-Cost Naturally Ventilated Maize Seed Storage System

A 22-m3 residential room was converted to a seed storage facility by retrofitting a solar collector on the roof. Three different chimney sizes of diameter and height of 200 mm x 3.6 m, 200 mm x 4.8 m, 300 mm x 3.6 m, and 300 mm x 4.8 m were investigated to determine the best size of the chimney to be used for ventilation in a storage facility. The parameters measured were the air velocity in the chimney duct, as well as the air temperature and relative humidity at the inlet, centre, and outlet of the storage facility. The diameter of the chimney had a significant effect (P<0.05) on the ventilation rate achieved in the storage facility. Significant differences were found between the different chimney diameters and heights (P≤0.05). The 300 mm diameter chimneys were able to extract hot air from the roof solar collector; however, the 200 mm diameter failed. The modified naturally-ventilated seed storage room was able to reduce the relative humidity from 69.7% to a safe relative humidity of 37.9%, while at the same time the temperature increased from 23.3℃ to 35℃ in the 300 mm x 4.8 m chimney.

Genetic diversity and potential inoculum sources of Fusarium species causing cankers in bareroot-propagated almond trees in California nurseries

Previous research determined that Fusarium acuminatum and Fusarium avenaceum are important causal agents of a canker disease in bareroot-propagated fruit and nut trees in California that emerges during cold-storage or after transplanting. The disease largely disappeared after 2001, but it reemerged in 2011 in almond trees in at least one nursery. This motivated further study of the etiology and epidemiology of the disease by undertaking studies to determine distribution of the pathogens throughout almond nursery propagation systems and trace possible sources of inoculum. Research initiated in 2013 detected pathogenic Fusarium spp. throughout the almond propagation system, including in healthy trees, in soils, on wheat rotation crops, on equipment, and in the cold storage facility air. In addition to the two Fusarium spp. implicated previously, Fusarium brachygibbosum and a new Fusarium species, Fusarium californicum, were found to be pathogenic on almond trees. Multi-locus sequence typing and somatic compatibility testing confirmed that isolates within a species collected from different materials in the nursery were all highly genetically similar and likely of one clonal lineage. These findings affirm that equipment surfaces, wheat rotation crops, soil, cold storage facility air, and asymptomatic almond tree materials (i.e., rootstock cuttings, budwood, and scions) can potentially contribute inoculum to increase disease prevalence and severity.

RESEARCH OF THE POSSIBILITY OF TRANSMISSION OF LIQUEFIED NATURAL GAS THROUGH A PIPELINE BY A NON-PRESSURE METHOD

This article discusses the problems of transmission of liquefied natural gas through a non-pressure pipeline from a stationary storage facility to a transport cryogenic tank and ways to solve them. Theoretical studies have been carried out, including mathematical modeling of thermal and hydrodynamic processes during the transmission of liquefied natural gas through a pipeline by a non-pressure method.

Consolidated nuclear waste storage in Andrews, Texas: An integrated technical and policy risk analysis

Over the last 50 years, nuclear energy has reduced US energy-related CO2 emissions by over 30 gigatons compared to if the same electricity were produced by fossil fuels such as coal and natural gas. However, many kilotons of spent nuclear fuel have accumulated at different sites across the country, and sociopolitical factors have frustrated efforts to address the challenge of nuclear waste disposal. Presently, a consolidated interim storage facility in Andrews, Texas, provides a promising temporary solution. In this paper, we compare the technical and policy risks of the project to continued storage at independent spent fuel storage installations. Our results indicate that the cost of the radiological risk is low (<$30,000) for both scenarios. However, policy and societal considerations will impact the viability of the proposed consolidated interim storage facility. The safety and suitability of this interim storage facility will be affected by when a permanent repository becomes available, whether insurance for offsite waste storage is available, and the impact of climate risks. Although a consolidated interim storage facility at Andrews can potentially serve as a safe and economically advantageous solution, we highlight why these concerns must be addressed for the successful implementation of this facility, and more broadly for the future of the US nuclear industry.

Simulating the feedback between corrosive gas generation and water availability for the evaluation of radionuclide mobility in the context of radioactive waste disposal

It has been recently recognized that the availability of liquid water may be a controlling factor in the feedback between the physical processes of variably saturated liquid and gas flow on the one hand, and various chemical processes such as metal corrosion in an underground storage facility for radioactive waste on the other hand (e.g., Huang et al., 2021, and reference therein). Iron corrosion in anoxic conditions produces hydrogen gas and consumes water, as expressed by the following stylized chemical equation (e.g., Diercks and Kassner, 1988; Senior et al., 2021): 3Fe+4H2O⟶Fe3O4+4H2 Since water is an educt the corrosion reaction may be suspended or suppressed by the scarcity of water near the corroding surfaces. At the same time, gas pressure build-up through hydrogen generation may limit further water ingress. We developed a model that focuses on the close coupling between gas generation through iron corrosion and water availability. The feedback between iron corrosion, gas generation and liquid phase flow is considered by implementing the corrosion reaction in the subsurface flow and transport simulator PFLOTRAN (Hammond et al., 2012; Lichtner et al., 2015, 2020) making use of its coding provisions to implement source/sink terms for water and gas. These source/sink terms reflect the kinetics of the iron corrosion and its dependence on the educts, where the availability of water is approximated by the local liquid saturation. The model was applied to evaluate the mobility of radionuclides in, and their release from a hypothetical geological storage facility for radioactive waste. The radionuclides are traced through the emplacement chambers and drift by means of advective and diffusive transport. Parameter variations illustrate the influence of crucial modelling parameters on the simulation results.

Comparative Analysis of Marketing Efficiencies of Paddy and Locally Milled Rice (Oryza sativaL.) Marketers in the Federal Capital Territory, Nigeria

paddy and locally milled rice marketers in the Federal Capital Territory, Nigeria. It specifically examined the marketing efficiencies and factors influencing marketing efficiencies of paddy and locally milled rice marketers in the study area. Primary data were collected using well-structured pre-tested questionnaires while a multistage sampling technique was adopted to obtain responses from respondents. Descriptive (mean, frequency and percentages) and inferential statistics (shepherd’s index and multiple regression analysis) were used to analyze the data. In using shepherd’s index, the results revealed 78% and 77% marketing efficiencies for paddy and locally milled rice respectively while the multiple regression analysis revealed sex, household size, availability of storage facility and contract marketing arrangement were significant to marketing efficiency of paddy rice; while membership of a market association, cost of purchasing rice, selling price of rice, availability of storage facility and contract arrangement were significant to marketing efficiency of locally milled rice. The study concludes that paddy rice marketers are more efficient in the study area and therefore recommends the initiation of more contract arrangements between investors and marketers in the area, while marketers should be encouraged to join marketing associations to take advantage of the endless benefits to be gained. It also recommends the formulation of policies that favour inclusivity of youths and women in the trade.