Purification of goats' milk casein by reversed-phase high-performance liquid chromatography and identification of αs1-casein
1987 ◽
Vol 54
(3)
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pp. 361-367
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Keyword(s):
SummaryGoats’ milk caseins were separated into four components in a single run using reversed-phase gradient high-performance liquid chromatography. The purity of the isolated components was checked by sodium dodecyl sulphate polyacrylamide gel electrophoresis, amino acid analysis and determination of the N-terminal residue. By a comparison with previously published results for goats’ milk caseins the four peaks were identified as κ-, αs1-, αs2- and β-casein. In order to confirm the existence of αsl-casein in goats’ milk, this component was sequenced for 44 steps, revealing a sequence homologous to bovine αsl-casein and almost identical to the N-terminal sequence previously published by Boulanger et al. (1984).
1992 ◽
Vol 573
(1)
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pp. 17-22
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1987 ◽
Vol 54
(2)
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pp. 199-205
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2010 ◽
Vol 28
(2)
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pp. 204-208
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