The miracidium of Transversotrema patialense (Soparkar, 1924)

1994 ◽  
Vol 68 (1) ◽  
pp. 49-51 ◽  
Author(s):  
R. Madhavi ◽  
K. Jhansilakshmibai
Keyword(s):  
Epidermal Cells ◽  
Penetration Gland ◽  
Apical Papilla

AbstractThe miracidium of Transversotrema patialense (Digenea: Transversotrematidae) has 21 epidermal cells arranged in four tiers with an epidermal formula of 6, 9, 4, 2. The penetration gland is large, conspicuous, contains a posterior globular part with 6 prominent nuclei and opens out through 6 pores arranged in two lateral groups at the tip of the apical papilla. The miracidium lacks lateral papillae, possesses a well developed subepithelium, a pair of flame cells and a germ ball.

Composition, secretion, and fate of the glands in the miracidium and sporocyst of Fasciola hepatica L.

1983 ◽  
Vol 57 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Gerald R. Buzzell
Keyword(s):  
Fasciola Hepatica ◽  
Leading Edge ◽  
Snail Host ◽  
Epidermal Cells ◽  
Positive Material ◽  
Apical Papilla

ABSTRACTThe anterior end of the miracidium of Fasciola hepatica contains a large flask-shaped apical gland and four unicellular lateral glands, all of which have ducts which pass to the tip of the apical papilla. These glands appear to be involved in penetration of the larva into the snail host. The apical gland secretes as the miracidium proves the epidermis of the host before attachment. It seems likely that its secretion is a chemical which lyses the epidermal cells. The lateral glands are PAS-positive and may contain a neutral mucopolysaccharide. They also secrete as the miracidium probes the snail and a layer of PAS-positive material may be seen at the leading edge of the apical papilla as the larva penetrates into the host. Both the apical gland and the lateral glands may be visible in the sporocyst for several days after penetration.

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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