Glutathione-s-transferase is an important antigen in the eel nematode Anguillicola crassus

1997 ◽  
Vol 71 (4) ◽  
pp. 319-324 ◽  
Author(s):  
M.E. Nielsen ◽  
K. Buchmann
Keyword(s):  
Specific Antibody ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Glutathione S Transferase ◽  
Elisa System ◽  
Humoral Immune ◽  
Anguillicola Crassus ◽  
Reducing Conditions

AbstractDifferent organs and secretions/excretions of the swimbladder parasite, Anguillicola crassus (Nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the European eel, Anguilla anguilla. Proteins from different fractions of Anguillicola crassus were separated using SDS–PAGE (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. Infected eels showed a specific antibody response to a 43 kDa antigen in the cuticle and towards two gonad antigens around 34 and 43 kDa. In protein released from the worms, two secretory/excretory antigens of approximately 28 kDa were found. The secretion/excretion rate of protein from the parasite to the surroundings was determined. Subsequently, an ELISA system was established applying these antigens as the first layer of coating. Furthermore, antigens from Anguillicola crassus were examined for the presence of glutathione-s-transferase (GST) using a specific antibody against GST. The antigens were found to be subunits of GST.

An enhanced humoral immune response against the swimbladder nematode, Anguillicola crassus, in the Japanese eel, Anguilla japonica, compared with the European eel, A. anguilla

1999 ◽  
Vol 73 (3) ◽  
pp. 227-232 ◽  
Author(s):  
M.E. Nielsen
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Plasma Levels ◽  
Anguilla Anguilla ◽  
Anguilla Japonica ◽  
Control Fish ◽  
European Eel ◽  
Lower Plasma ◽  
Humoral Immune ◽  
Anguillicola Crassus

The humoral immune response in the two eel species, Anguilla japonica and Anguilla anguilla against two fractions of antigens in Anguillicola crassus were studied.Within species, both eel species showed significantly elevated titres compared with controls when immunized with antigens from Anguillicola crassus.In interspecific comparison, Anguilla japonicashowed significantly elevated titres in comparison with Anguilla anguilla. Immunization of Anguilla anguillacaused a significantly decrease in the plasma levels of protein in comparison with control fish and all groups of Anguilla japonica. In contrast, Anguilla japonica showed significantly lower plasma levels of Ig in all groups compared with Anguilla anguilla.The different susceptibilities to Anguillicola crassus between the natural host, Anguilla japonica, and the naı¨ve, Anguilla anguilla, is partly due to differences in the ability of the two eel species to mount a humoral immune response.

Effects of experimental infections with different doses of Anguillicola crassus (Nematoda, Dracunculoidea) on European eel (Anguilla anguilla)

Aquaculture ◽  
1996 ◽  
Vol 141 (1-2) ◽  
pp. 41-57 ◽  
Author(s):  
O.L.M. Haenen ◽  
T.A.M. van Wijngaarden ◽  
M.H.T. van der Heijden ◽  
J. Höglund ◽  
J.B.J.W. Cornelissen ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Experimental Infections ◽  
Anguillicola Crassus ◽  
Different Doses

Atrial natriuretic peptide receptors in renal papilla of DOCA-salt hypertensive rats

1990 ◽  
Vol 259 (1) ◽  
pp. F130-F137
Author(s):  
E. Nuglozeh ◽  
G. Gauquelin ◽  
R. Garcia ◽  
J. Tremblay ◽  
E. L. Schiffrin
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Plasma Renin ◽  
Renal Papilla ◽  
Hypertensive Rats ◽  
Reducing Conditions ◽  
Anp Receptors ◽  
Atrial Natriuretic

The receptor for atrial natriuretic peptide (ANP) in the rat renal papilla was characterized pharmacologically. After solubilization and irreversible binding with disuccinimidylsuberate, it was shown on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) to be made of a single peptide of 125 kDa. The regulation of the renal papillary ANP receptor was studied in deoxycorticosterone acetate (DOCA)-salt hypertensive rats. DOCA-salt rats had suppressed plasma renin activity and increased plasma ANP concentrations (408 +/- 35 vs. 133 +/- 12 pg/ml in uninephrectomized controls, P less than 0.01). The renal papilla was hypertrophied in DOCA-salt hypertensive rats (93 +/- 1 vs. 52 +/- 1 mg, P less than 0.01). The density of ANP sites in the papilla was significantly higher in DOCA-salt rats (141 +/- 31 fmol/papilla) than in controls (34 +/- 8 fmol/papilla, P less than 0.01). Affinity of sites in DOCA-salt rats and controls was similar. The production of guanosine 3',5'-cyclic monophosphate (cGMP) in renal papilla in response to ANP was significantly higher in DOCA-salt rats. In contrast to the renal papillary ANP receptor, acid-washed vascular and glomerular ANP sites were significantly decreased in density in DOCA-salt hypertensive rats. In blood vessels and glomeruli, both the high- and low-molecular mass receptor (as detected on SDS-PAGE under reducing conditions) was proportionately decreased in density in DOCA-salt hypertensive rats. The present results suggest that an increased number of ANP receptors and exaggerated cGMP response to ANP in the renal papilla may underlie the increased natriuretic responsiveness of the kidney to ANP in DOCA-salt hypertensive rats.

scholarly journals The effect of foraging and ontogeny on the prevalence and intensity of the invasive parasite Anguillicola crassus in the European eel Anguilla anguilla

2017 ◽  
Vol 40 (9) ◽  
pp. 1213-1222 ◽  
Author(s):  
J Barry ◽  
M Newton ◽  
J A Dodd ◽  
D Evans ◽  
J Newton ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Anguillicola Crassus
Sign in / Sign up

Export Citation Format

Share Document