An enhanced humoral immune response against the swimbladder nematode, Anguillicola crassus, in the Japanese eel, Anguilla japonica, compared with the European eel, A. anguilla

1999 ◽  
Vol 73 (3) ◽  
pp. 227-232 ◽  
Author(s):  
M.E. Nielsen
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Plasma Levels ◽  
Anguilla Anguilla ◽  
Anguilla Japonica ◽  
Control Fish ◽  
European Eel ◽  
Lower Plasma ◽  
Humoral Immune ◽  
Anguillicola Crassus

The humoral immune response in the two eel species, Anguilla japonica and Anguilla anguilla against two fractions of antigens in Anguillicola crassus were studied.Within species, both eel species showed significantly elevated titres compared with controls when immunized with antigens from Anguillicola crassus.In interspecific comparison, Anguilla japonicashowed significantly elevated titres in comparison with Anguilla anguilla. Immunization of Anguilla anguillacaused a significantly decrease in the plasma levels of protein in comparison with control fish and all groups of Anguilla japonica. In contrast, Anguilla japonica showed significantly lower plasma levels of Ig in all groups compared with Anguilla anguilla.The different susceptibilities to Anguillicola crassus between the natural host, Anguilla japonica, and the naı¨ve, Anguilla anguilla, is partly due to differences in the ability of the two eel species to mount a humoral immune response.

Glutathione-s-transferase is an important antigen in the eel nematode Anguillicola crassus

1997 ◽  
Vol 71 (4) ◽  
pp. 319-324 ◽  
Author(s):  
M.E. Nielsen ◽  
K. Buchmann
Keyword(s):  
Specific Antibody ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Glutathione S Transferase ◽  
Elisa System ◽  
Humoral Immune ◽  
Anguillicola Crassus ◽  
Reducing Conditions

AbstractDifferent organs and secretions/excretions of the swimbladder parasite, Anguillicola crassus (Nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the European eel, Anguilla anguilla. Proteins from different fractions of Anguillicola crassus were separated using SDS–PAGE (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. Infected eels showed a specific antibody response to a 43 kDa antigen in the cuticle and towards two gonad antigens around 34 and 43 kDa. In protein released from the worms, two secretory/excretory antigens of approximately 28 kDa were found. The secretion/excretion rate of protein from the parasite to the surroundings was determined. Subsequently, an ELISA system was established applying these antigens as the first layer of coating. Furthermore, antigens from Anguillicola crassus were examined for the presence of glutathione-s-transferase (GST) using a specific antibody against GST. The antigens were found to be subunits of GST.

scholarly journals Immersion Vaccination by a Biomimetic-Mucoadhesive Nanovaccine Induces Humoral Immune Response of Red Tilapia (Oreochromis sp.) against Flavobacterium columnare Challenge

Vaccines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1253
Author(s):  
Sirikorn Kitiyodom ◽  
Teerapong Yata ◽  
Kim D. Thompson ◽  
Janina Costa ◽  
Preetham Elumalai ◽  
...  
Keyword(s):  
Immune Response ◽  
Bactericidal Activity ◽  
Humoral Immune Response ◽  
Control Fish ◽  
Flavobacterium Columnare ◽  
Systemic Immune Response ◽  
Cumulative Mortality ◽  
Humoral Immune ◽  
Serum Bactericidal Activity ◽  
Columnaris Disease

Immersion vaccination with a biomimetic mucoadhesive nanovaccine has been shown to induce a strong mucosal immune response against columnaris disease, a serious bacterial disease in farmed red tilapia caused by Flavobacterium columnare. However, the induction of a systemic immune response by the vaccine is yet to be investigated. Here, we examine if a specific humoral immune response is stimulated in tilapia by a biomimetic-mucoadhesive nanovaccine against Flavobacterium columnare using an indirect-enzyme-linked immunosorbent assay (ELISA), serum bactericidal activity (SBA) and the expression of immune-related genes within the head-kidney and spleen, together with assessing the relative percent survival of vaccinated fish after experimentally infecting them with F. columnare. The anti-IgM antibody titer of fish at 14 and 21 days post-vaccination was significantly higher in chitosan complex nanoemulsion (CS-NE) vaccinated fish compared to fish vaccinated with the formalin-killed vaccine or control fish, supporting the serum bactericidal activity results at these time points. The cumulative mortality of the unvaccinated control fish was 87% after challenging fish with the pathogen, while the cumulative mortality of the CS-NE vaccinated group was 24%, which was significantly lower than the formalin-killed vaccinated and control fish. There was a significant upregulation of IgM, IgT, TNF α, and IL1-β genes in the spleen and kidney of vaccinated fish. Significant upregulation of IgM and IgT genes was observed in the spleen of CS-NE vaccinated fish. The study confirmed the charged-chitosan-based mucoadhesive nanovaccine to be an effective platform for immersion vaccination of tilapia, with fish generating a humoral systemic immune response against columnaris disease in vaccinated fish.

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