F.6 The immune response of european eel (Anguilla anguilla) after infection with Anguillicola crassus (nematoda, dracunculoidea)

1994 ◽  
Vol 18 ◽  
pp. S103
Keyword(s):  
Immune Response ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Anguillicola Crassus

An enhanced humoral immune response against the swimbladder nematode, Anguillicola crassus, in the Japanese eel, Anguilla japonica, compared with the European eel, A. anguilla

1999 ◽  
Vol 73 (3) ◽  
pp. 227-232 ◽  
Author(s):  
M.E. Nielsen
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Plasma Levels ◽  
Anguilla Anguilla ◽  
Anguilla Japonica ◽  
Control Fish ◽  
European Eel ◽  
Lower Plasma ◽  
Humoral Immune ◽  
Anguillicola Crassus

The humoral immune response in the two eel species, Anguilla japonica and Anguilla anguilla against two fractions of antigens in Anguillicola crassus were studied.Within species, both eel species showed significantly elevated titres compared with controls when immunized with antigens from Anguillicola crassus.In interspecific comparison, Anguilla japonicashowed significantly elevated titres in comparison with Anguilla anguilla. Immunization of Anguilla anguillacaused a significantly decrease in the plasma levels of protein in comparison with control fish and all groups of Anguilla japonica. In contrast, Anguilla japonica showed significantly lower plasma levels of Ig in all groups compared with Anguilla anguilla.The different susceptibilities to Anguillicola crassus between the natural host, Anguilla japonica, and the naı¨ve, Anguilla anguilla, is partly due to differences in the ability of the two eel species to mount a humoral immune response.

Effects of experimental infections with different doses of Anguillicola crassus (Nematoda, Dracunculoidea) on European eel (Anguilla anguilla)

Aquaculture ◽  
1996 ◽  
Vol 141 (1-2) ◽  
pp. 41-57 ◽  
Author(s):  
O.L.M. Haenen ◽  
T.A.M. van Wijngaarden ◽  
M.H.T. van der Heijden ◽  
J. Höglund ◽  
J.B.J.W. Cornelissen ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Experimental Infections ◽  
Anguillicola Crassus ◽  
Different Doses

scholarly journals The effect of foraging and ontogeny on the prevalence and intensity of the invasive parasite Anguillicola crassus in the European eel Anguilla anguilla

2017 ◽  
Vol 40 (9) ◽  
pp. 1213-1222 ◽  
Author(s):  
J Barry ◽  
M Newton ◽  
J A Dodd ◽  
D Evans ◽  
J Newton ◽  
...  
Keyword(s):  
Anguilla Anguilla ◽  
European Eel ◽  
Anguillicola Crassus

Glutathione-s-transferase is an important antigen in the eel nematode Anguillicola crassus

1997 ◽  
Vol 71 (4) ◽  
pp. 319-324 ◽  
Author(s):  
M.E. Nielsen ◽  
K. Buchmann
Keyword(s):  
Specific Antibody ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Glutathione S Transferase ◽  
Elisa System ◽  
Humoral Immune ◽  
Anguillicola Crassus ◽  
Reducing Conditions

AbstractDifferent organs and secretions/excretions of the swimbladder parasite, Anguillicola crassus (Nematoda), were tested for the presence of antigens to the humoral immune response previously detected in the European eel, Anguilla anguilla. Proteins from different fractions of Anguillicola crassus were separated using SDS–PAGE (sodium-dodecyl-sulphate polyacrylamide-gel electrophoresis) under reducing conditions and electroblotted onto nitrocellulose membranes. Infected eels showed a specific antibody response to a 43 kDa antigen in the cuticle and towards two gonad antigens around 34 and 43 kDa. In protein released from the worms, two secretory/excretory antigens of approximately 28 kDa were found. The secretion/excretion rate of protein from the parasite to the surroundings was determined. Subsequently, an ELISA system was established applying these antigens as the first layer of coating. Furthermore, antigens from Anguillicola crassus were examined for the presence of glutathione-s-transferase (GST) using a specific antibody against GST. The antigens were found to be subunits of GST.

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