scholarly journals A serological study of Haemophilus influenzae

1937 ◽  
Vol 37 (1) ◽  
pp. 98-107 ◽  
Author(s):  
A. E. Platt
Keyword(s):  
Haemophilus Influenzae ◽  
Type A ◽  
Small Sample ◽  
Partial Purification ◽  
Serological Study ◽  
Serological Tests ◽  
Carbohydrate Fraction ◽  
Smooth Strain ◽  
Type Strains ◽  
Antigenic Heterogeneity

1. Of eighty-six strains of Haem. influenzae isolated from the naso-pharynx of normal persons, sixteen had the general characters of Pittman's “smooth” type.2. These sixteen strains and one “smooth” strain isolated from a case of meningitis were submitted to various serological tests, including the separation and partial purification of a carbohydrate fraction from the meningeal strain and from four of the sixteen strains.3. By precipitin tests carried out with these purified fractions, it was possible to identify three of the five “smooth” naso-pharyngeal strains and the meningeal strain, as belonging to Pittman's type e, and one of the “smooth” naso-pharyngeal strains to Pittman's type a.4. Some indication of grouping, within this small sample of smooth strains, was obtained by various other methods, such as precipitin tests carried out with simple saline washings or agglutination reactions; but, apart from the four type strains a, b, e and f, received from Miss Pittman, no strain could be satisfactorily typed by any of these methods. Only by using partially purified polysaccharide fractions was it possible to assign any of the “smooth” nasopharyngeal strains to their correct type.5. The testing of these naso-pharyngeal strains against antisera prepared against types a, b, e and f by direct agglutination, showed that either many of these strains contained some proportion of the smooth antigens, or that the antisera contained antibodies acting on other antigenic components, although, in relation to the type strains themselves, they appeared to be specific.6. The examination of the eighty-six strains, as a whole, revealed the extreme antigenic heterogeneity that has been noted by many previous workers.

scholarly journals In vitro immunological activities of the polysaccharide fraction from Haemophilus influenzae type a endotoxin.

1982 ◽  
Vol 36 (2) ◽  
pp. 603-608 ◽  
Author(s):  
M Guenounou ◽  
D Raichvarg ◽  
D Hatat ◽  
C Brossard ◽  
J Agneray
Keyword(s):  
Haemophilus Influenzae ◽  
Type A ◽  
Haemophilus Influenzae Type ◽  
Polysaccharide Fraction

scholarly journals Case report of congenital asplenia presenting with Haemophilus influenzae type a (Hia) sepsis: an emerging pediatric infection in Minnesota

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Tiffany Albrecht ◽  
Kristina Poss ◽  
Satja Issaranggoon Na Ayuthaya ◽  
Lori Triden ◽  
Katherine L. Schleiss ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
American Indian ◽  
Bacterial Infections ◽  
Type A ◽  
Invasive Disease ◽  
Haemophilus Influenzae Type ◽  
Uneventful Recovery ◽  
Antibiotic Administration ◽  
Indian Children ◽  
Disease Manifestation

Abstract Background In the pre-vaccine era, invasive disease with Haemophilus influenzae, type b (Hib) commonly presented with osteoarticular involvement. Haemophilus influenzae, type a (Hia) sepsis is a rare but emerging problem in recent years. Here, we report a case of sepsis with concomitant osteoarthritis due to Hia that was the presenting infectious disease manifestation of isolated asplenia in a young child. This unique observation adds to our understanding of sepsis and asplenia in children. Case presentation A five-year-old girl developed acute Hia bacteremia and sepsis. The patient developed arthritis shortly after onset of septic shock. Arthrocentesis was culture-negative, but given the difficulty differentiating between septic and reactive arthritis, prolonged antibiotic administration was provided for presumed osteoarticular infection, and the patient had an uneventful recovery. The finding of Howell-Jolly bodies on blood smear at the time of presentation prompted an evaluation that revealed isolated congenital asplenia. Evaluation for known genetic causes of asplenia was unrevealing. Investigation by the Minnesota Department of Health revealed an emergence of Hia infections over the past 5 years, particularly in children with an American Indian background. Conclusions Hia is an important pathogen in the differential diagnosis of invasive bacterial infections in children and shares overlap in clinical presentation and pathogenesis with Hib. Invasive Hia disease can be a presenting manifestation of asplenia in children. Hia is an emerging pathogen in American Indian children.

Non‐vaccine Haemophilus influenzae type a epiglottitis

2020 ◽  
Author(s):  
Sulaiman Almuzam ◽  
Daniel Lin ◽  
Gail Wong ◽  
David Isaacs ◽  
Julie Huynh
Keyword(s):  
Haemophilus Influenzae ◽  
Type A ◽  
Haemophilus Influenzae Type

scholarly journals Invasive disease caused by Haemophilus influenzae type a in Northern Ontario First Nations communities

2011 ◽  
Vol 60 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Len Kelly ◽  
Raymond S. W. Tsang ◽  
Alanna Morgan ◽  
Frances B. Jamieson ◽  
Marina Ulanova
Keyword(s):  
Haemophilus Influenzae ◽  
First Nations ◽  
Type A ◽  
Invasive Disease ◽  
Indigenous Populations ◽  
Haemophilus Influenzae Type ◽  
Northern Ontario ◽  
Northwestern Ontario ◽  
Genotypic Characteristics ◽  
Increased Susceptibility

Seven epidemiologically unrelated cases of invasive Haemophilus influenzae type a (Hia) disease were identified in First Nations communities of Northwestern Ontario, Canada, in 2004–2008. In all cases, Hia was isolated from blood. The clinical presentation in most of the cases was moderately severe and all patients responded to antibiotic therapy. Laboratory analysis of Hia isolates from Northwestern Ontario indicated striking similarities in their phenotypic and genotypic characteristics. The findings are discussed in the context of current epidemiology of invasive Hia disease. Our data along with some published studies by others suggest an increased susceptibility to this infection among North American indigenous populations.

A 20-Kilodalton N-Terminal Fragment of the D15 Protein Contains a Protective Epitope(s) against Haemophilus influenzae Type a and Type b

1998 ◽  
Vol 66 (7) ◽  
pp. 3349-3354 ◽  
Author(s):  
Yan-ping Yang ◽  
Wayne R. Thomas ◽  
Pele Chong ◽  
Sheena M. Loosmore ◽  
Michel H. Klein
Keyword(s):  
Haemophilus Influenzae ◽  
Protective Antigen ◽  
Cell Epitope ◽  
Type A ◽  
Laboratory Animals ◽  
Haemophilus Influenzae Type ◽  
Type B ◽  
Infant Rats ◽  
Terminal Fragment ◽  
Protective Epitope

ABSTRACT A conserved 80-kDa minor outer membrane protein, D15, ofHaemophilus influenzae has been shown to be a protective antigen in laboratory animals against H. influenzae type a (Hia) or type b (Hib) infection. To localize the protective B-cell epitope(s) within the D15 protein and to further explore the possibility of using synthetic peptides as vaccine antigens, a 20-kDa N-terminal fragment of D15 protein (truncated D15 [tD15]) was expressed as a fusion protein with glutathioneS-transferase in Escherichia coli. The tD15 moiety was cleaved from glutathione S-transferase by using thrombin and purified to homogeneity. The purified soluble tD15 appeared to contain immunodominant protective epitope(s) against Hia and Hib, since rabbit antisera directed against tD15 were capable of protecting infant rats from Hia or Hib bacteremia. The ease of purification of soluble tD15, therefore, makes it a better candidate antigen than the full-length recombinant D15 which is produced as inclusion bodies in E. coli. Furthermore, both the purified tD15 fragment and a mixture of tD15-derived peptides spanning amino acid residues 93 to 209 of the mature D15 protein were capable of inhibiting the protection against Hib conferred on infant rats by rabbit anti-tD15 antiserum, indicating that the protective epitopes of D15 may not be conformational. However, the administration of pooled rabbit immune sera raised against the same panel of peptides failed to protect infant rats from Hib infection.

scholarly journals Prevalence and Distribution of Adhesins in Invasive Non-Type b Encapsulated Haemophilus influenzae

2003 ◽  
Vol 71 (4) ◽  
pp. 1635-1642 ◽  
Author(s):  
Carina A. Rodriguez ◽  
Vasanthi Avadhanula ◽  
Amy Buscher ◽  
Arnold L. Smith ◽  
Joseph W. St. Geme ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Red Blood Cells ◽  
Gene Cluster ◽  
Blood Cells ◽  
Genetic Relatedness ◽  
Type A ◽  
Respiratory Epithelium ◽  
Major Type ◽  
Type B

ABSTRACT Adhesion to the respiratory epithelium plays an important role in Haemophilus influenzae infection. The distribution of H. influenzae adhesins in type b and nontypeable strains has been characterized, but little is known about the prevalence of these factors in non-type b encapsulated strains. We analyzed 53 invasive type a, type e, and type f strains for the presence of hap, hia, hmw, and hif genes; Hap, Hia, and HMW1/2 adhesins; and hemagglutinating pili. The hap gene was ubiquitous, and homologs of hmw and hia were present in 7 of 53 (13.2%) and 45 of 53 (84.9%) strains, respectively. Hap was detected in 28 of 45 (62.2%) hap+ strains, HMW1/2 was detected in 5 of 7 (71.4%) hmw+ strains, and Hia was detected in 31 of 45 (68.8%) hia+ strains. The hif gene cluster was present in 26 of 53 strains (49.1%), and 21 of 26 hif+ strains (80.8%) agglutinated (HA) red blood cells. Nine isolates exhibited HA but lacked the hif gene cluster. The distribution of adhesin genes correlated with the genetic relatedness of the strains. Strains belonging to one type a clonotype and the major type e clonotype possessed hia but lacked the hif cluster. Strains belonging to the second type a clonotype possessed both hia and hif genes. All type f strains belonging to the major type f clonotype possessed hia and lacked hifB. Although the specific complement of adhesin genes in non-type b encapsulated H. influenzae varies, most invasive strains express Hap and Hia, suggesting these adhesins may be especially important to the virulence of these organisms.

Fatal Haemophilus influenzae type a sepsis in an infant

2012 ◽  
Vol 49 (3) ◽  
pp. E235-E238 ◽  
Author(s):  
Joshua Francis ◽  
Marc Anders ◽  
Pam Lobegeier ◽  
Clare Nourse
Keyword(s):  
Haemophilus Influenzae ◽  
Type A ◽  
Haemophilus Influenzae Type

scholarly journals A Serological Study of Undulant Fever in Southern Rhodesia

1927 ◽  
Vol 26 (4) ◽  
pp. 403-419 ◽  
Author(s):  
G. R. Ross
Keyword(s):  
Absorption Test ◽  
Serological Study ◽  
Serological Investigation ◽  
Southern Rhodesia ◽  
Serological Difference ◽  
The One ◽  
Type Strains

1. Examination of nine type strains of Brucella, collected from various sources, by agglutination and the absorption of agglutinin test revealed distinct serological difference between Br. abortus and Br. melitensis. Differentiation could only be made by the agglutinin-absorption test, both organisms agglutinating equally with abortus or melitensis immune sera.2. By agglutination alone differentiation could be made between a group consisting of Br. melitensis and Br. abortus strains on the one hand and a group which included one strain labelled Br. melitensis, one labelled Br. abortus, and Br. paramelitensis.3. The agglutinin-absorption test showed that this Br. paramelitensis group comprised two strains of closely allied paramelitensis strains, and that the Br. abortus strain could be regarded as a Br. para-abortus strain.4. Serological investigation of eight strains of Brucella isolated from patients suffering from undulant fever in Southern Rhodesia showed that six were serologically identical with type Br. abortus and two identical with what is regarded as a Br. para-abortus strain.5. It is suggested that the melitensis-abortus group represent the “S” normal types, whereas the paramelitensis-para-abortus group represent the “R” mutant types of organisms.

Sign in / Sign up

Export Citation Format

Share Document