scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Comparison of the results of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 365-371 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Comparison Of The Results ◽  
The Rose ◽  
Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

scholarly journals The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests

1982 ◽  
Vol 88 (1) ◽  
pp. 11-19 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes ◽  
B. A. Rogerson ◽  
L. J. Shenfield
Keyword(s):  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Serological Response ◽  
Serum Samples ◽  
Plate Test ◽  
The Rose ◽  
Serum Agglutination ◽  
Haemolysis Test

SummarySerum samples were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests.The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions.In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera.Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

scholarly journals Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis

2012 ◽  
Vol 56 (4) ◽  
pp. 467-471 ◽  
Author(s):  
Marcin Weiner ◽  
Wojciech Iwaniak ◽  
Krzysztof Szulowski
Keyword(s):  
False Positive ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Specific Antibodies ◽  
Serological Examination ◽  
Culture Positive ◽  
Serum Agglutination ◽  
Fluorescence Polarisation ◽  
Porcine Brucellosis

Abstract The aim of this study was the evaluation of fluorescence polarisation assay (FPA) in the diagnosis of porcine brucellosis in comparison with Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), 2-mercaptoethanol test, and ELISA. Eight hundred seventeen sera from pigs, including 612 sera from healthy animals, seven sera from Brucella suis bv2 culture positive animals, and 198 sera classified as false positive, originated from confirmatory investigations, were used. All sera from healthy animals, negative in RBT, SAT, CFT, and ELISA were also negative in FPA. All sera positive in serological examination, originated from Brucella infected animals, were also positive in FPA. Among sera classified as false positive almost half of the samples tested (49.49%) reacted positively in FPA. The examinations confirmed the usefulness of FPA in diagnosis of porcine brucellosis, but the method, like the other tests, does not allow resolving the problem of discrimination cross-reacting from specific antibodies.

scholarly journals Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

1983 ◽  
Vol 90 (1) ◽  
pp. 67-70 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Supporting Evidence ◽  
Bovine Brucellosis ◽  
Eradication Programme ◽  
Female Cattle ◽  
The Rose ◽  
Supplementary Test ◽  
Haemolysis Test

SUMMARYSera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

Acute phase biomarkers, oxidants, antioxidants, and trace minerals of mobile sheep flocks naturally infected with brucellosis

2021 ◽  
Vol 24 (4) ◽  
pp. 559-573
Author(s):  
N. A. Shalby ◽  
A. M. Abo El-Maaty ◽  
A. H. Ali ◽  
M. Elgioushy
Keyword(s):  
Ascorbic Acid ◽  
Acute Phase ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Trace Minerals ◽  
Predictive Test ◽  
Serological Tests ◽  
Blood Concentrations ◽  
Blood Biochemical

This study assayed the acute phase responses of sheep seropositive to Brucella. Sera collected from ewes (n=160) were subjected to serological tests of Brucella, Rose Bengal plate agglutination test (RBPAT), buffer acidified plate agglutination test (BAPAT), and complement fixation test (CFT). Results revealed that CFT was the most predictive test of brucellosis followed by BAPAT then RBPAT. The moderate predictive blood biochemical parameters were zinc and ascorbic acid. Ewes with low CFT titre (chronic) had low fibrinogen, copper, NO, and GPx. Seropositive animals had high blood concentrations of ascorbic acid and zinc.

The porcine brucellosis – evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions

2013 ◽  
Vol 16 (1) ◽  
pp. 129-130 ◽  
Author(s):  
M. Weiner ◽  
K. Szulowski ◽  
W. Iwaniak
Keyword(s):  
Yersinia Enterocolitica ◽  
False Positive ◽  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bacteriological Examination ◽  
Serum Agglutination ◽  
Porcine Brucellosis

Abstract Forty four pigs with typical characteristics for false positive serological reactions (FPSR) were examined for the presence of Yersinia enterocolitica O:9. The positive reactions were observed in rose bengal test (RBT, N=23 sera), serum agglutination test (SAT, N=16), complement fixation test (CFT, N=9), indirect ELISA (i-ELISA, N=11) in first, and in RBT (N=14), SAT (N=8), CFT (N=7) and i-ELISA (N=18) in second examination, respectively. In bacteriological examination Y. enterocolitica was confirmed in 12 cases. Six of these isolates were identified with PCR as Y. enterocolitica O:9.

scholarly journals Verification of Sera from Cattle and Pigs for Brucellosis with Fluorescence Polarisation Assay

2013 ◽  
Vol 57 (2) ◽  
pp. 157-160 ◽  
Author(s):  
Marcin Weiner ◽  
Wojciech Iwaniak ◽  
Krzysztof Szulowski ◽  
Monika Szymajda
Keyword(s):  
Rose Bengal ◽  
False Positive ◽  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Agglutination Test ◽  
The Other ◽  
Serum Agglutination ◽  
Fluorescence Polarisation ◽  
Potential Tool

Abstract Fluorescence polarisation assay (FPA) was evaluated as a potential tool improving specificity of serological diagnosis of brucellosis in cattle and pigs. The evaluation was performed by comparing the results of FPA with the results of rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test, and indirect ELISA when problematic sera, regarded as false positive, were tested. Four hundred and seventy-five sera, including 201 porcine and 274 bovine samples, reacting positively in at least one classical serological assay were used. Only six bovine sera were FPA positive (two positive in SAT and RBT and four positive in SAT only). Different situation was observed when porcine sera were examined. Out of 201 sera, 109 were also positive in FPA. The studies confirmed that in cattle FPA enables to reduce highly the number of false positive reactions for brucellosis. On the other hand, in pigs, the method is a little more specific in comparison to other methods applied.

Sign in / Sign up

Export Citation Format

Share Document