Acute phase biomarkers, oxidants, antioxidants, and trace minerals of mobile sheep flocks naturally infected with brucellosis

2021 ◽  
Vol 24 (4) ◽  
pp. 559-573
Author(s):  
N. A. Shalby ◽  
A. M. Abo El-Maaty ◽  
A. H. Ali ◽  
M. Elgioushy
Keyword(s):  
Ascorbic Acid ◽  
Acute Phase ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Trace Minerals ◽  
Predictive Test ◽  
Serological Tests ◽  
Blood Concentrations ◽  
Blood Biochemical

This study assayed the acute phase responses of sheep seropositive to Brucella. Sera collected from ewes (n=160) were subjected to serological tests of Brucella, Rose Bengal plate agglutination test (RBPAT), buffer acidified plate agglutination test (BAPAT), and complement fixation test (CFT). Results revealed that CFT was the most predictive test of brucellosis followed by BAPAT then RBPAT. The moderate predictive blood biochemical parameters were zinc and ascorbic acid. Ewes with low CFT titre (chronic) had low fibrinogen, copper, NO, and GPx. Seropositive animals had high blood concentrations of ascorbic acid and zinc.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Comparison of the results of some serological tests for bovine brucellosis

1978 ◽  
Vol 80 (3) ◽  
pp. 365-371 ◽  
Author(s):  
R. J. Chappel ◽  
D. J. McNaught ◽  
J. A. Bourke ◽  
G. S. Allan
Keyword(s):  
Rose Bengal ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Comparison Of The Results ◽  
The Rose ◽  
Serum Agglutination

SummaryA total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

scholarly journals Model Proficiency Testing Scheme for Serological Diagnosis of Brucellosis: Interlaboratory Study

2004 ◽  
Vol 87 (4) ◽  
pp. 965-971 ◽  
Author(s):  
Donatella Nannini ◽  
Manuela Tittarelli ◽  
Lucilla Ricci ◽  
Annamaria Conte ◽  
Bernardo Di Emidio ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reference Laboratory ◽  
Correct Result ◽  
The European Union ◽  
Serological Tests ◽  
Testing Scheme ◽  
Z Scores ◽  
Qualitative Test

Abstract A model interlaboratory testing scheme was developed by the Italian National Reference Laboratory for Brucellosis. This scheme was planned for both qualitative (Rose Bengal Plate Test; RBPT) and quantitative (Complement Fixation Test; CFT) serological tests and involved a total of 42 laboratories. In the preparation of this scheme, reference was made to general protocols and guidelines and to methods reported in the literature, which were applicable to analytical chemistry laboratories. Six field sera from naturally infected animals, one positive serum at a titer below the European Union (EU) positivity threshold, and 5 sera positive at titers between 20 and 851 International Units of Complement Fixation Test (IUCFT)/mL plus one negative serum were used to produce a panel of test sera. To evaluate laboratory performances in the quantitative test for each tested sample examined, z-scores based on robust summary statistics (the median and normalized interquartile range) were used. To evaluate overall laboratory performance, 2 types of combined z-scores were used: Rescaled Sum of Scores and Sum of Squared Scores. In the case of the qualitative test (RBPT), results were analyzed by a Bayesian approach. A Beta distribution, based on the result of each laboratory, was calculated and used to estimate the probability of each laboratory giving a correct result and its uncertainty.

scholarly journals Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis)

2006 ◽  
Vol 75 (2) ◽  
pp. 988-996 ◽  
Author(s):  
Rosanna Capparelli ◽  
Flora Alfano ◽  
Maria Grazia Amoroso ◽  
Giorgia Borriello ◽  
Domenico Fenizia ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Untranslated Region ◽  
Complement Fixation Test ◽  
Water Buffalo ◽  
Complement Fixation ◽  
Bubalus Bubalis ◽  
Lower Number ◽  
Agglutination Test ◽  
Intracellular Bacteria ◽  
Antibacterial Protein

ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

scholarly journals Detection of brucellosis in cattle and evaluation of risk factors associated with the disease in workers slaughterhouse

2021 ◽  
Vol 10 (3) ◽  
pp. e39710313248
Author(s):  
Thaise Marques Alves ◽  
Poliana de Castro Melo ◽  
Lilia Marcia Paulin Silva ◽  
Nathana Kyolla Santos de Carvalho ◽  
Amora Ferreira Menezes Rios ◽  
...  
Keyword(s):  
Risk Factors ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
State Inspection ◽  
Agglutination Test ◽  
The State ◽  
Antigen Test ◽  
Factors Associated ◽  
Inspection Service ◽  
Smooth Strain

Brucellosis is anthropozoonosis caused by Brucella spp. Among the zoonotic species, B. abortus is the main species affecting cattle and can easily be transmitted to humans. The purpose of this study was to investigate, through epidemiological inquiry and serological analysis,  animal and human health as related to smooth strain Brucella spp. in a slaughterhouse located in the southern region of the state of Bahia. For this purpose, blood samples were collected from workers and animals at a slaughterhouse together with the State Inspection Service. Then, the Buffered Acidified Plate Antigen test was performed for animals and humans, the Slow Agglutination Test was performed for humans only; and the Complement Fixation Test and the 2-Mercaptoethanol Brucella Agglutination test (2ME) were performed for animals only. In addition, an epidemiological inquiry was applied to workers in order to assess risk factors for the disease. After data analysis, it was concluded that infection by smooth strains of Brucella spp. was detected in 14.0% of the cattle. Additionally, one worker out 41 tested reactive to the disease.

scholarly journals A Century of syphilis serology

2007 ◽  
Vol 28 (1) ◽  
pp. 25
Author(s):  
Peter W Robertson
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Congenital Syphilis ◽  
Serological Test ◽  
Serological Diagnosis ◽  
Serological Tests ◽  
Western Blots ◽  
Comprehensive Review ◽  
The Subject ◽  
Syphilis Serology

The year 2006 represented the centenary of the first diagnostic serological test, the Wasserman complement fixation test for the diagnosis of syphilis. In this article I will relate some developments I have observed in the day-to-day running of a diagnostic serology laboratory over the thirty years preceding this centenary. I have assumed some background knowledge and elected to discuss only selected aspects of serological tests for syphilis (STS), rather than attempt a comprehensive review of the subject. A number of areas such as the serological diagnosis of congenital syphilis, the use of and interpretation of western blots in ?problem? sera and diagnosis of other treponemal diseases have not been included.

scholarly journals Development of new antigens for serologic diagnosis of animal trypanosomosis and their use for epizootic monitoring

2021 ◽  
Vol 15 (1) ◽  
pp. 71-78
Author(s):  
Ch. Georgiou
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Test System ◽  
Fixation Test ◽  
Federal State ◽  
State Budget ◽  
Serological Tests ◽  
Hemagglutination Test ◽  
Indirect Hemagglutination ◽  
Indirect Hemagglutination Test

The purpose of the research is developing a method for obtaining erythrocyte antigens containing and not containing Trypanosoma equiperdum and T. evansi DNA, which can later be used in serological reactions to differentiate these types of Trypanosoma.Materials and methods. The studies were conducted in the Protozoology Laboratory and the Vyshnevolotsk Branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV RAS”, as well as livestock farms of the Russian Federation and other countries using clinical, microscopic, hematological, parasitological, biomolecular and serological methods.Results and discussion. Studies carried out for the first time have shown that it is possible to use erythrocyte antigens containing the T. equiperdum and T. evansi DNA obtained after 3-fold administration to mice and rabbits of a mixture of trypanosomal antigen with addition of 1.0 ml of an adjuvant (aluminum hydroxide), and bleeding of animals at 25 to 30 days. The formed precipitate was used as an antigen for serological tests. Experiments have shown that blood for preparation of positive serum can be taken when antibodies are in titers of 1:20 in the Prolonged Complement Fixation Test, and at least 1:400 in the Indirect Hemagglutination Test and ELISA, and for negative serum when horse blood serum reacts negatively with antigens of T. equiperdum and T. evansi in the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA. The test systems of the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA prepared by us with antigens containing and not containing T. equiperdum and T. evansi DNA resulted in creating a universal test system (Indirect Hemagglutination Test) for differentiating T. equiperdum from T. evansi.

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