scholarly journals Preparation of Inorganic and Organic Carbon for 14C Analysis from a Single Marine Sample

Radiocarbon ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Sheila Griffin ◽  
Ellen R. M. Druffel

We have developed a technique using a single apparatus to recover the inorganic and organic carbon from a small (few milligrams) aliquot of dried marine material for radiocarbon analysis. The main advantages of using a single apparatus are: 1) less sample is required, 2) decreased handling reduces contamination, and 3) less time and materials are used. Blank values of ∼5 μg and 19–44 μg are obtained for the inorganic and organic carbon extractions, respectively. δ14C results from sinking particulate organic and inorganic carbon are presented for samples collected in deep-sea sediment traps deployed for 10–30 day periods at 650 and 100 m above bottom (mab) in the northeast Pacific Ocean.

2020 ◽  
Vol 47 (11) ◽  
Author(s):  
Ponnusamy Saravanan ◽  
Anil K. Gupta ◽  
Hongbo Zheng ◽  
Santosh K. Rai ◽  
Mruganka K. Panigrahi

2021 ◽  
Vol 10 (34) ◽  
Author(s):  
Emilie J. Skoog ◽  
Julie A. Huber ◽  
Margrethe H. Serres ◽  
Alice Levesque ◽  
Lisa Zeigler Allen

A thermophilic chemolithoautotrophic bacterium was isolated from vent fluids at Axial Seamount, an active deep-sea volcano in the northeast Pacific Ocean. We present the draft genome sequence of Desulfurobacterium sp. strain AV08.


Radiocarbon ◽  
2013 ◽  
Vol 55 (3) ◽  
pp. 1651-1658 ◽  
Author(s):  
Chanda Bertrand ◽  
Brett Walker ◽  
Sheila Griffin ◽  
E R M Druffel

It has long been assumed that radiocarbon (Δ14C) content of dissolved inorganic carbon (DIC) is equal to that of particulate organic carbon (POC) in surface seawater; however, little research has been conducted to explicitly test this assumption. Here, we report Δ14C measurements of surface POC samples and compare them with contemporaneous DIC Δ14C measurements from the northeast Pacific Ocean (Hwang et al. 2004; Druffel et al. 2010). Samples were collected from surface waters at Station M off California between 1995 and 2004. The POC Δ14C values decreased 3.2% per year from 1995 to 2004, similar to the decline observed in the DIC Δ14C values during the same period. Overall, our results show no statistical difference between POC and DIC Δ14C—consistent with the assumption that DIC and POC Δ14C values can generally be considered equivalent. However, significant variability was observed for POC Δ14C values during several fall/summer events, where POC Δ14C signatures were lower than DIC Δ14C values. An evaluation of 2 sample pretreatments also suggests that non-homogenized POC samples deviated less from average POC Δ14C values and more closely matched the DIC Δ14C average for the time series. The presence of seasonal POC/DIC Δ14C disagreements, combined with sample processing effects, suggest that infrequent contributions of allochthonous, older carbon may have originated from deeper in the water column, especially during periods when upwelling in this area was prominent.


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