Ultrastructure of Intranuclear Round Bodies of Enta-Moeba Histolytica After Dnasa and Rnasa Treatment

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072939 ◽

1973 ◽

Vol 31 ◽

pp. 498-499

Author(s):

A. Gonzàlez Angulo ◽

I. Ruiz de Chàvez ◽

N. Treviño

Keyword(s):

Life Cycle ◽

Electron Density ◽

Functional Role ◽

Dna And Rna ◽

Morphological Studies ◽

Membrane Bound ◽

Electron Density Averaging

Recent morphological studies of E. histolytica have revealed the presence of several intranuclear structures which are clearly distinguished from other nuclear components. These are round membrane bound bodies of variable marked electron density averaging from 15 to 40 milimicra in diameter (Fig. 1). Their intranuclear location and persistance within the nucleus along the various stages of the life cycle of E. histolytica seems to indicate that these structures may have a functional role in the nucleus. The present study was undertaken to determine the existence of DNA and RNA in this intranuclear structures.Trophozoites of E. histolytica ABRM strains obtained from monoxenic cultures were fixed for 35 minutes in 2.5% Glutaraldehyde, 0.1M sodium cacodilate buffer.

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The functional role of sodium taurocholate cotransporting polypeptide NTCP in the life cycle of hepatitis B, C and D viruses

Cellular and Molecular Life Sciences ◽

10.1007/s00018-018-2892-y ◽

2018 ◽

Vol 75 (21) ◽

pp. 3895-3905 ◽

Cited By ~ 5

Author(s):

Carla Eller ◽

Laura Heydmann ◽

Che C. Colpitts ◽

Eloi R. Verrier ◽

Catherine Schuster ◽

...

Keyword(s):

Life Cycle ◽

Hepatitis B ◽

Functional Role ◽

Sodium Taurocholate

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Studies on the possible relationships of microbodies and multivesicular bodies to oxalate, endopolygalacturonase, and cellulase (Cx) production by Sclerotinia sclerotiorum

Canadian Journal of Botany ◽

10.1139/b72-216 ◽

1972 ◽

Vol 50 (8) ◽

pp. 1743-1748 ◽

Cited By ~ 14

Author(s):

Douglas P. Maxwell ◽

Paul H. Williams ◽

Martha D. Maxwell

Keyword(s):

Sclerotinia Sclerotiorum ◽

Enzyme Production ◽

Functional Role ◽

Carbon Sources ◽

Extracellular Enzyme ◽

Ultrastructural Organization ◽

Multivesicular Bodies ◽

Membrane Bound ◽

High Production

The possible functional role of vesicles and crystal-containing microbodies in the production of oxalate, endopolygalacturonase, or cellulase by Sclerotinia sclerotiorum was investigated. The presence of multivesicular bodies in hyphal tips was not correlated with secretion or production of oxalate or these extracellular hydrolases. More crystal-containing microbodies were present in hyphal tips grown on media which supported greater extracellular enzyme production. No correlation existed between numbers of crystal-containing microbodies in hyphal tips and production of oxalate. Numerous membrane-bound vesicles (0.09–0.18 µm diam) were associated with tips grown on a D-glucose–Na succinate medium which supported high production of oxalate. The general ultrastructural organization of these hyphal tips was similar to that reported for other ascomycetes. Differences in numbers and distributions of organelles were observed between hyphal tips and older hyphae as well as between hyphal tips grown on the different carbon sources.

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Early Lesions of Proliferative Enteritis in Pigs and Hamsters

Veterinary Pathology ◽

10.1177/030098588902600311 ◽

1989 ◽

Vol 26 (3) ◽

pp. 260-264 ◽

Cited By ~ 27

Author(s):

S. McOrist ◽

G. H. K. Lawson ◽

A. C. Rowland ◽

N. MacIntyre

Keyword(s):

Life Cycle ◽

Intestinal Lumen ◽

Intracellular Bacteria ◽

Immunogold Staining ◽

Specific Antibodies ◽

Early Lesions ◽

Membrane Bound ◽

Gnotobiotic Pigs ◽

Intracellular Multiplication ◽

Marked Proliferation

Gnotobiotic pigs and conventional hamsters were given suspensions of intestinal mucosa from a pig with proliferative hemorrhagic enteropathy and killed 10 or 21 days later. Affected animals had evidence of marked proliferation of immature enterocytes in the intestinal crypts. Numerous Campylobacter-like organisms were in the cytoplasm of enterocytes, and in some instances, bacteria were closely associated with enter-ocytes. Some intracellular bacteria lying below the microvillous border were within membrane-bound structures. Immunofluorescence and electron immunogold staining with specific antibodies indicated that these organisms were antigenically different from curved bacteria in the crypt lumen of early lesions. This study indicates that the life cycle of the intracellular organisms may involve entry into crypt enterocytes from the intestinal lumen with subsequent intracellular multiplication.

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Functional role of membrane-bound adenylyl cyclases and coupled to them receptors and G-proteins in regulation of fertility of spermatozoa

Journal of Evolutionary Biochemistry and Physiology ◽

10.1134/s0022093014040024 ◽

2014 ◽

Vol 50 (4) ◽

pp. 286-302

Author(s):

A. O. Shpakov ◽

K. V. Derkach

Keyword(s):

G Proteins ◽

Functional Role ◽

Adenylyl Cyclases ◽

Membrane Bound

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A membrane-bound plastid inclusion in the epidermis of leaves of Taraxacum officinale

Canadian Journal of Botany ◽

10.1139/b77-031 ◽

1977 ◽

Vol 55 (2) ◽

pp. 222-225 ◽

Cited By ~ 3

Author(s):

E. S. Martin ◽

G. Larbalestier

Keyword(s):

Inclusion Bodies ◽

Functional Role ◽

Taraxacum Officinale ◽

Single Membrane ◽

Membrane Bound ◽

Dense Inclusion ◽

Electron Dense Inclusion

Epidermal chloroplasts of Taraxacum officinale agg. contain large electron-dense inclusion bodies enclosed by a single membrane. These inclusion bodies were not observed in mesophyll chloroplasts. The origin and functional role of these structures is discussed.

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Software for handling macromolecular envelopes

Acta Crystallographica Section D Biological Crystallography ◽

10.1107/s0907444999001031 ◽

1999 ◽

Vol 55 (4) ◽

pp. 941-944 ◽

Cited By ~ 110

Author(s):

G. Jacob Kleywegt ◽

Thomas A. Jones

Keyword(s):

Electron Density ◽

Local Density ◽

Computer Programs ◽

Real Space ◽

Atomic Model ◽

Density Correlation ◽

Phase Extension ◽

Crystallographic Symmetry ◽

Averaging Techniques ◽

Electron Density Averaging

Macromolecular phase-refinement and phase-extension calculations using real-space electron-density averaging techniques require accurate envelopes (or masks) to define the boundaries of each domain or molecule whose density is to be averaged. An extensive set of tools, implemented in four computer programs (O, MAMA, COMA and MASKIT) are described which can be used to generate such envelopes (either from an atomic model or based on local density-correlation maps), to improve them, to remove overlap owing to crystallographic or non-crystallographic symmetry, to display them and to manipulate them in a variety of manners.

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