scholarly journals The development and evaluation of a μ-capture ELISA detecting chlamydia-specific Igm

1988 ◽  
Vol 101 (2) ◽  
pp. 387-395 ◽  
Author(s):  
T. G. Wreghitt ◽  
V. J. Robinson ◽  
E. O Caul ◽  
I. D. Paul ◽  
S. Gatley
Keyword(s):  
Respiratory Tract ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Capture Elisa ◽  
The Past ◽  
Heat Stable ◽  
Antibody Conjugate

SUMMARYA μ-capture enzyme-linked immunosorbent assay (ELISA) for detecting chlamydia-specific IgM was developed by use of the heat stable, lipopolysaccharide group-specific antigen and an alkaline phosphatase-labelled anti-chlamydia group-specific monoclonal antibody conjugate. The test was used to study the serological response in chlamydial respiratory tract infection among patients with acute respiratory tract symptoms in Cambridgeshire during the past 7 years. Results were compared with those of the complement fixation test (CFT) in routine use as well as those of a whole inclusion indirect immunofluorescence (WIF) test for IgM. Correlation between results of the μ-capture ELISA and those of the WIF test was 87·5%.

Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. III. The antibody response to infestation with Trichostrongylus spp.

1950 ◽  
Vol 1 (4) ◽  
pp. 413 ◽  
Author(s):  
DF Stewart
Keyword(s):  
Antibody Response ◽  
Haemonchus Contortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Immunological Response ◽  
Serological Response ◽  
Apparent Effect ◽  
Relative Resistance ◽  
Infective Larvae ◽  
Immunological Reactions

In infestation of sheep with Trichostrongylus spp., both the intake of larvae and infestation with adult worms stimulated the production of antibodies. The character of the antibody response in infestation with Trichostrongylus spp. thus differed from that caused by Haemonchus contortus. Sera of sheep dying from infestation with Trichostrongylus spp. were negative to the complement fixation test. It was found that older sheep responded serologically earlier and more vigorously to infestation with Trichostrongylus spp. than did young sheep. Subsequent doses of larvae of Trichostrongylus spp. elicited a more rapid and grester serological response, even in young sheep, than an initial dose of larvae. Previous infestation with H. contortus did not result in resistance to Trichostrongylus spp., whereas previous infestation with Trichostrongylus spp. heightened the resistance of sheep to subsequent infestation with the same species. It is concluded that the relative resistance of older sheep to Trichostrongylus spp. is due, a t least in part, to an earlier immunological response which is strengthened by subsequent doses of larvae. When infective larvae of Trichostrongylus spp. were superimposed upon an existing infestation of the same species, the egg count declined ;apidly and the development of the superimposed larvae resisted Doses of infective larvae of Trichostrongylus spp. had no apparent effect on the egg counts of sheep infested with H. contortus.

STUDIES ON COMPLEMENT FIXING ANTIGENS OF LEPTOSPIRAE

1960 ◽  
Vol 6 (4) ◽  
pp. 453-462 ◽  
Author(s):  
N. A. Labzoffsky ◽  
A. E. Kelen
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Specific Antigen ◽  
Cross Reaction ◽  
Fixation Test ◽  
Specific Antibodies ◽  
Cross Reactions ◽  
Homologous Antigen ◽  
Precise Diagnosis ◽  
Specific Antigens

Methods of preparation of "whole" and type-specific antigens from leptospiral cultures for use in the complement fixation test are outlined. Leptospiral cultures grown in Korthof's medium were treated with pyridine and after appearance of a copious precipitate were centrifuged. The supernatants were dialyzed and after concentration by pervaporation were used as "whole" antigens. Type-specific antigens were prepared from acetone precipitates of filtrates of "whole" antigens. Considerable cross reaction was observed with "whole" antigens prepared from L. pomona, L. canicola, and L. icterohaemorrhagiae and their respective rabbit hyperimmune sera, although titers with homologous sera were invariably higher. These cross reactions, however, were not as pronounced with sera from cattle naturally infected with L. pomona, where approximately 43% of the sera reacted with homologous antigen only. No reactions were observed between the type-specific antigen and any of the heterologous sera employed. It has also been shown that with experimental sera type-specific antibodies appeared earlier and persisted longer than group-specific antibodies. In view of the above observations the use of polyvalent antigen for routine screening of leptospiral antisera is advocated. Type-specific antigens are recommended for use in more precise diagnosis.

scholarly journals The role of the spirochaete in the Wassermann reaction

1939 ◽  
Vol 39 (3) ◽  
pp. 298-310 ◽  
Author(s):  
A. Beck
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Rockefeller Foundation ◽  
Specific Antigen ◽  
The Other ◽  
Specific Absorption ◽  
Homologous Antigen ◽  
The Difference ◽  
Wassermann Reaction

1. The examination of 1100 sera by both the Wassermann reaction and the complement-fixation test with spirochaetes revealed a superior sensitivity of the latter reaction and practically equal specificity of the two tests.2. Syphilitic serum contains two different antibodies: one reacting with the lipoid antigen of the Wassermann reaction, the other with a specific antigen in the spirochaete.3. The spirochaetal antibody of syphilitic serum has a complex serological structure, corresponding to spirochaete strains of different antigenic make-up.4. The existence of this antibody and its specific absorption by the homologous antigen can also be demonstrated by agglutination.5. The difference between agglutinin titres found in normal and syphilitic sera is not pronounced enough to render this method satisfactory for the practical diagnosis of syphilis.6. The spirochaete contains, apart from its specific antigen, the ubiquitous lipoid substance representing the Wassermann antigen.7. A fraction was obtained from spirochaetes by Raistrick and Topley's method which in complement-fixation and precipitation tests reacted actively with spirochaete antisera from rabbits, but which so far failed to react with syphilitic sera.This work was carried out with the aid of a grant from the Rockefeller Foundation. I wish to thank Prof. Golla, Director of the Central Pathological Laboratory, L.C.C. Mental Hospitals, who rendered this work possible, and Dr Arthur Davies, Director of the Devonport Laboratory, for the hospitality afforded me at his laboratory and for the patients’ sera used in this work. I am indebted to Prof. R. T. Hewlett for his revision of the manuscript, to Prof. Raistrick for advice in chemical matters, and to Dr Amies, of the Lister Institute, for his help with the Sharples centrifuge.

NEW COMPLEMENT FIXING ANTIGEN FOR SERODIAGNOSIS OF GONORRHOEA

1961 ◽  
Vol 7 (5) ◽  
pp. 715-723
Author(s):  
N. A. Labzoffsky ◽  
A. E. Kelen
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Laboratory Diagnosis ◽  
Fixation Test ◽  
New Method ◽  
Strain Selection ◽  
The Past

A new method of preparing gonococcal antigen for use in the complement fixation test is presented. Briefly, the procedure consists of extracting Neisseria gonorrhoeae cells with pyridine and then exposing the washed sediment to ultrasonic treatment.The new method is superior to that of Price by yielding highly potent, genus-specific and stable antigens, which are free of anticomplementary properties and possess a broader antigenic valence and a longer range of working antigenic power. It is simpler and more practical than Torrey's modification of the Price method since the lengthy and laborious procedure of strain selection is eliminated.In routine use for the past several years, the new antigen has proved to be specific and reliable in the complement fixation test. The test itself provides a useful supplementary aid to the laboratory diagnosis of gonorrhoea.

Studies on the Serological Response in Sheep to Naturally Acquired Gastro-intestinal Nematodes. I. Preparation of Antigens and Evaluation of Serological Techniques

1956 ◽  
Vol 30 (2-3) ◽  
pp. 129-142 ◽  
Author(s):  
E. J. L. Soulsby
Keyword(s):  
Low Temperature ◽  
Antibody Titre ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Cross Reactivity ◽  
Serological Response ◽  
High Antibody ◽  
Passive Haemagglutination ◽  
Haemagglutination Test ◽  
Antibody Titres

Antigens were prepared by low temperature methods to preserve “functional” antigen. These antigens showed marked cross reactivity and were satisfactory in complement fixation tests.The passive haemagglutination test using “functional” antigen gave titres which were higher than those obtained with the complement fixation test. High antibody titres were observed in sheep naturally infested with nematodes and a relationship between antibody titre and egg count was demonstrated.

Sign in / Sign up

Export Citation Format

Share Document