scholarly journals Q fever in the Netherlands: a sero-epidemiological survey among human population groups from 1968 to 1983

1987 ◽  
Vol 98 (2) ◽  
pp. 211-219 ◽  
Author(s):  
J. H. Richardus ◽  
A. Donkers ◽  
A. M. Dumas ◽  
G. J. P. Schaap ◽  
J. P. W. M. Akkermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Indirect Immunofluorescence ◽  
Q Fever ◽  
Epidemiological Survey ◽  
Immunofluorescence Test ◽  
Age Classes ◽  
Serum Samples ◽  
Occupational Groups ◽  
Indirect Immunofluorescence Test ◽  
Long Time

SUMMARYA sero-epidemiological survey, using an indirect immunofluorescence test for IgG against Coxiella burnetii (phase II), was carried out in the Netherlands. Serum samples taken in 1968, 1975, 1979 and 1983 were tested. Occupational groups with a supposedly high risk of infection (veterinarians, residents of dairy farms and taxidermists) showed a significantly higher percentage of seropositives than defined controls. The percentage of seropositive amateur wool spinners was significantly higher than that of the controls from the same region. Since 1968 there has been no increase in the percentage of infected persons, indicating that, contrary to earlier assumptions, Q fever has been endemic in The Netherlands for a long time already. The increase in numbers of notified cases of overt Q fever is considered to be the result of the recent introduction of a sensitive indirect immunofluorescence test for IgM antibodies against C. burnetii. Antibody percentages in all age classes between 1 and 64 years were much alike, suggesting that most infections occur in early childhood. This is in accordance with the finding that 35% of our patients are younger than 3 years. The possibility of infection related to childbirth and lactation is discussed.

Seroepidemiology of Q fever in New Brunswick and Manitoba

1988 ◽  
Vol 34 (9) ◽  
pp. 1043-1045 ◽  
Author(s):  
Thomas J. Marrie
Keyword(s):  
Phase I ◽  
New Brunswick ◽  
Antibody Titer ◽  
Phase Ii ◽  
Coxiella Burnetii ◽  
Blood Donors ◽  
Q Fever ◽  
Immunofluorescence Test ◽  
Serum Samples ◽  
Indirect Immunofluorescence Test

A seroepidemiological survey, using an indirect immunofluorescence test, was carried out on serum samples obtained from New Brunswick and Manitoba blood donors during 1986. The antigens were Coxiella burnetii phase I and phase II from strain Nine Mile. Eighty of the 503 (15.9%) Manitoba blood donors had a phase II antibody titer of ≥ 1:8, while 41 (4.2%) of the 966 New Brunswick blood donors had such antibodies. We have recently diagnosed three cases of Q fever in New Brunswick but none have been diagnosed in Manitoba. Our data suggest that Q fever may be increasing in New Brunswick and repeated seroepidemiological studies are indicated. It is likely that undetected cases of Q fever are occurring in Manitoba.

Recognition of a Non-HLA-ABC Antigen Present on B and T Lymphocytes and Monocytes only Detectable with the Indirect Immunofluorescence Test

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (3) ◽  
pp. 150-158
Author(s):  
F. Décary ◽  
F.W.A. Verheugt ◽  
L. van Helden-Henningheim ◽  
E. von Riesz ◽  
R. Schreuder-van Gelder ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Antigen Present ◽  
B And T Lymphocytes

scholarly journals Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

1987 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Amauri Braga Simonetti ◽  
Jorge Teixeira
Keyword(s):  
Cerebrospinal Fluid ◽  
Indirect Immunofluorescence ◽  
False Positive ◽  
Clinical Evidence ◽  
Routine Analysis ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunofluorescent Test ◽  
Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

Evaluation of the automated indirect immunofluorescence test for anti-dsDNA antibodies

2019 ◽  
Vol 498 ◽  
pp. 143-147 ◽  
Author(s):  
Shiji Wu ◽  
Feng Wang ◽  
Jin Huang ◽  
Botao Yin ◽  
Min Huang ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Dsdna Antibodies

Correlation between enzyme immunoassay and indirect immunofluorescence test for high titer of anti-ribosomal-P antibodies

2012 ◽  
Vol 413 (5-6) ◽  
pp. 650-652 ◽  
Author(s):  
Kyeong-Hee Kim ◽  
Bo-Ram Kim ◽  
Ji-Eun Kim ◽  
Kwang-Sook Woo ◽  
Jin-Yeong Han ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
High Titer ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Ribosomal P

Epidemiological survey of classical swine fever in Tibetan pigs in Nyingchi, Tibet China

2016 ◽  
Author(s):  
Jia-Kui Li ◽  
Hui Zhang ◽  
Peng Shang ◽  
Yangzom Chamba
Keyword(s):  
Classical Swine Fever ◽  
Epidemiological Survey ◽  
Economic Losses ◽  
Serum Samples ◽  
Immune Effect ◽  
Tibetan Pigs ◽  
Significant Difference ◽  
Long Time ◽  
The Government

Classical swine fever (CSF) is a major hazardous disease to the pigs and as a dangerous epidemic; it causes a serious economic losses to the pig industry. Though, a national compulsory immunization of CSF vaccines had been carried out for a long time, scarce information can be got about the immune effect of CSF in Tibetan pigs. The present study was to investigate the seroprevalence of CSF in Tibetan pigs in Nyingchi area of Tibet, China. A total 454 samples were collected from November 2014 to January 2015 and were investigated through enzyme-linked immuno sorbent assay (ELISA). The results showed that 241 (53.1%, 95% CI 48.4-57.8) pigs were found to be positive for CSF with the further distribution of 53.3% (95% CI 46.8–59.6), 49.5% (95% CI 42.2-56.8) and 93.8% (95% CI 69.8-99.8) in Tibetan counties of Nyingchi, Mainling and Gongbo'gyamda, respectively. There was no significant difference in male (52.8%, 95% CI 46.4-59.1) and female pigs (50.0%, 95% CI 42.6-57.4). Though, 53.1% of the serum samples were tested out positive to CSF, only the seroprevalence of CSF in Tibetan pigs in Gongbo'gyamda were higher than 70% which was ruled by the government. The low seroprevalence of CSF in Tibetan pigs should arise a serious concern and effective methods should be taken, in order to prevent CSF infection effectively.

Therapy of Alveococcosis in Man

1977 ◽  
Vol 5 (5) ◽  
pp. 367-368 ◽  
Author(s):  
C E Lüdin ◽  
K Gyr ◽  
K Karoussos
Keyword(s):  
Partial Hepatectomy ◽  
Indirect Immunofluorescence ◽  
The Other ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Passive Haemagglutination ◽  
Indirect Immunofluorescence Test ◽  
Reliable Methods

Diagnostic, therapeutic and prognostic aspects are interpreted on the basis of two cases of Echinococcus alveolaris. Both patients had undergone partial hepatectomy and were subsequently treated chemotherapeutically. In one case dehydroemetine and mebendazole (Vermox®, Janssen), and in the other case metrifonate (Bilarcil®, Bayer A. G.) were used. The diagnosis of alveococcosis of the liver was demonstrated in one case histologically on the occasion of appendectomy, and in the other case by the indirect immunofluorescence test and passive haemagglutination. In case of suspected alveococcosis these serological tests are imperative, as they are the most reliable methods of demonstrating this disease. The latest trend in therapy aims at a combination of surgery and chemotherapy. Mebendazole seems to be promising as an anthelmintic agent for the treatment of alveococcosis. Remission of the disease was obtained in either case.

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