scholarly journals Phylogenetic analysis of Bunyamwera and Ngari viruses (family Bunyaviridae, genus Orthobunyavirus) isolated in Kenya

2015 ◽  
Vol 144 (2) ◽  
pp. 389-395 ◽  
Author(s):  
C. ODHIAMBO ◽  
M. VENTER ◽  
O. LWANDE ◽  
R. SWANEPOEL ◽  
R. SANG
Keyword(s):  
Human Disease ◽  
Rift Valley Fever ◽  
Temporal Association ◽  
Haemorrhagic Fever ◽  
Valley Fever ◽  
Genetic Sequences ◽  
Negative Sense ◽  
Virus Isolates ◽  
Bunyamwera Virus

SUMMARYOrthobunyaviruses, tri-segmented, negative-sense RNA viruses, have long been associated with mild to severe human disease in Africa, but not haemorrhagic fever. However, during a Rift Valley fever outbreak in East Africa in 1997–1998, Ngari virus was isolated from two patients and antibody detected in several others with haemorrhagic fever. The isolates were used to identify Ngari virus as a natural Orthobunyavirus reassortant. Despite their potential to reassort and cause severe human disease, characterization of orthobunyaviruses is hampered by paucity of genetic sequences. Our objective was to obtain complete gene sequences of two Bunyamwera virus and three Ngari virus isolates from recent surveys in Kenya and to determine their phylogenetic positioning within the Bunyamwera serogroup. Newly sequenced Kenyan Bunyamwera virus isolates clustered closest to a Bunyamwera virus isolate from the same locality and a Central African Republic isolate indicating that similar strains may be circulating regionally. Recent Kenyan Ngari isolates were closest to the Ngari isolates associated with the 1997–1998 haemorrhagic fever outbreak. We observed a temporal/geographical relationship among Ngari isolates in all three gene segments suggesting a geographical/temporal association with genetic diversity. These sequences in addition to earlier sequences can be used for future analyses of this neglected but potentially deadly group of viruses.

scholarly journals Rift Valley fever MP-12 vaccine Phase 2 clinical trial: Safety, immunogenicity, and genetic characterization of virus isolates

Vaccine ◽  
2016 ◽  
Vol 34 (4) ◽  
pp. 523-530 ◽  
Author(s):  
Phillip R. Pittman ◽  
Sarah L. Norris ◽  
Elizabeth S. Brown ◽  
Manmohan V. Ranadive ◽  
Barbara A. Schibly ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Genetic Characterization ◽  
Phase 2 ◽  
Valley Fever ◽  
Phase 2 Clinical Trial ◽  
Virus Isolates

scholarly journals Development and characterization of a Rift Valley fever virus cell–cell fusion assay using alphavirus replicon vectors

Virology ◽  
2006 ◽  
Vol 356 (1-2) ◽  
pp. 155-164 ◽  
Author(s):  
Claire Marie Filone ◽  
Mark Heise ◽  
Robert W. Doms ◽  
Andrea Bertolotti-Ciarlet
Keyword(s):  
Cell Fusion ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Cell Cell

Genetic Characterization of Rift Valley Fever Virus in Insectivorous Bats, Egypt

2021 ◽  
Vol 21 (12) ◽  
pp. 1003-1006
Author(s):  
Omar Sayed Saeed ◽  
Ayman Hany El-Deeb ◽  
Mohamed Rasheed Gadalla ◽  
Sherif Abdel Ghafar El-Soally ◽  
Hussein Aly Hussein Ahmed
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Genetic Characterization ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Insectivorous Bats ◽  
Valley Fever

scholarly journals Characterization of RNA aptamers directed against the nucleocapsid protein of Rift Valley fever virus

2012 ◽  
Vol 93 (3) ◽  
pp. 330-339 ◽  
Author(s):  
Mary Ellenbecker ◽  
Leila Sears ◽  
Ping Li ◽  
Jean-Marc Lanchy ◽  
J. Stephen Lodmell
Keyword(s):  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Rna Aptamers ◽  
Valley Fever

Molecular and biochemical studies of the evolution, infection and transmission of insect bunyaviruses

1988 ◽  
Vol 321 (1207) ◽  
pp. 463-483 ◽  
Keyword(s):  
Rift Valley Fever ◽  
Point Mutations ◽  
Molecular Techniques ◽  
Viral Gene ◽  
Gene Products ◽  
Haemorrhagic Fever ◽  
Valley Fever ◽  
Blood Sucking ◽  
Crimean Congo Haemorrhagic Fever ◽  
Genetic Level

Members of the Bunyaviridae family of RNA viruses (bunyaviruses, hantaviruses, nairoviruses, phleboviruses and uukuviruses) have been studied at the molecular and genetic level to understand the basis of their evolution and infection in vertebrate and invertebrate (arthropod) hosts. With the exception of the hantaviruses, these viruses infect and are transmitted by a variety of blood-sucking arthropods (mosquitoes, phlebotomines, gnats, ticks, etc.). The viruses are responsible for infection of various vertebrate species, occasionally causing human disease, morbidity and mortality (e.g. Rift Valley fever, Crimean-Congo haemorrhagic fever, Korean haemorrhagic fever). Genetic and molecular analyses of bunyaviruses have established the coding assignments of the three viral RNA species and documented which viral gene products determine host range and virulence. Ecological studies, with molecular techniques, have provided evidence for bunyavirus evolution in nature through genetic drift (involving the accumulation of point mutations) and shift (RNA- segment reassortment).

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