Current Applications and Methods of Microvascular Corrosion Casting: A Review

1998 ◽  
Vol 4 (S2) ◽  
pp. 908-909
Author(s):  
A. Lametschwandtner ◽  
H. Aichhorn ◽  
B. Minnich
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Great Depth ◽  
Corrosion Casting ◽  
Corrosion Casts ◽  
Depth Of Focus ◽  
Subsequent Removal ◽  
Blood Capillaries ◽  
Qualitative And Quantitative ◽  
Scanning Electron

Casting of hollow spaces with solidifying materials and subsequent removal of surrounding tissues by corrosive alkali and acids and the inspection of the remaining casts by bare eyes or the dissecting microscope is an old anatomical technique.The introduction of polymerizing resins as casting materials which resulted in durable casts of even the smallest spaces (bile and blood capillaries) and the application of the scanning electron microscope with its high resolution and great depth of focus, enabled the qualitative and the quantitative analysis of the 3D-arrangement of tubular systems by means of their casts.Presently, scanning electron microscopy of microvascular corrosion casts is used to study growing, stable or regressing blood vessel systems under physiological (e.g. during development, wound healing, metamorphosis) and pathological (e.g. tumor angiogenesis) conditions in qualitative and quantitative terms.

Record Wear Studies by Scanning Electron Microscopy

1968 ◽  
Vol 26 ◽  
pp. 364-365
Author(s):  
M.D. Coutts ◽  
E.R. Levin ◽  
J.G. Woodward
Keyword(s):  
Electron Microscopy ◽  
Constant Velocity ◽  
Peak Velocity ◽  
Great Depth ◽  
Depth Of Focus ◽  
Sweep Frequency ◽  
Transmission Electron ◽  
Test Record ◽  
Lateral Mode ◽  
Scanning Electron

While record grooves have been studied by transmission electron microscopy with replica techniques, and by optical microscopy, the former are cumbersome and restricted and the latter limited by lack of depth of focus and resolution at higher magnification. With its great depth of focus and ease in specimen manipulation, the scanning electron microscope is admirably suited for record wear studies.A special RCA sweep frequency test record was used with both lateral and vertical modulation bands. The signal is a repetitive, constant-velocity sweep from 2 to 20 kHz having a duration and repetitive rate of approximately 0.1 sec. and a peak velocity of 5.5 cm/s.A series of different pickups and numbers of plays were used on vinyl records. One centimeter discs were then cut out, mounted and coated with 200 Å of gold to prevent charging during examination. Wear studies were made by taking micrographs of record grooves having 1, 10 and 50 plays with each stylus and comparing with typical “no-play” grooves. Fig. 1 shows unplayed grooves in a vinyl pressing with sweep-frequency modulation in the lateral mode.

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