A Nanocrystalline Hilbert-Phase Plate for Phase-Contrast Transmission Electron Microscopy of Amorphous Objects

2012 ◽  
Vol 18 (S2) ◽  
pp. 496-497
Author(s):  
D. Manuel ◽  
B. Gamm ◽  
H. Simon ◽  
E. Müller ◽  
W. Send ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
Transmission Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

A nanocrystalline Hilbert phase-plate for phase-contrast transmission electron microscopy

2014 ◽  
Vol 139 ◽  
pp. 29-37 ◽  
Author(s):  
M. Dries ◽  
S. Hettler ◽  
B. Gamm ◽  
E. Müller ◽  
W. Send ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
Transmission Electron

In-Focus Electrostatic Zach Phase Plate Imaging for Transmission Electron Microscopy with Tunable Phase Contrast of Frozen Hydrated Biological Samples

2014 ◽  
Vol 20 (1) ◽  
pp. 175-183 ◽  
Author(s):  
Nicole Frindt ◽  
Marco Oster ◽  
Simon Hettler ◽  
Björn Gamm ◽  
Levin Dieterle ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Structural Information ◽  
Signal To Noise Ratio ◽  
Phase Plate ◽  
Spatial Frequencies ◽  
Transmission Electron ◽  
Sample Orientation ◽  
Phase Plates

AbstractTransmission electron microscopy (TEM) images of beam sensitive weak-phase objects such as biological cryo samples usually show a very low signal-to-noise ratio. These samples have almost no amplitude contrast and instead structural information is mainly encoded in the phase contrast. To increase the sample contrast in the image, especially for low spatial frequencies, the use of phase plates for close to focus phase contrast enhancement in TEM has long been discussed. Electrostatic phase plates are favorable in particular, as their tunable potential will allow an optimal phase shift adjustment and higher resolution than film phase plates as they avoid additional scattering events in matter. Here we show the first realization of close to focus phase contrast images of actin filament cryo samples acquired using an electrostatic Zach phase plate. Both positive and negative phase contrast is shown, which is obtained by applying appropriate potentials to the phase plate. The dependence of phase contrast improvement on sample orientation with respect to the phase plate is demonstrated and single-sideband artifacts are discussed. Additionally, possibilities to reduce contamination and charging effects of the phase plate are shown.

Multi-pore carbon phase plate for phase-contrast transmission electron microscopy

2014 ◽  
Vol 146 ◽  
pp. 91-96 ◽  
Author(s):  
Takumi Sannomiya ◽  
Juliane Junesch ◽  
Fumio Hosokawa ◽  
Kuniaki Nagayama ◽  
Yoshihiro Arai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
Carbon Phase ◽  
Transmission Electron

scholarly journals A Nanocrystalline Hilbert Phase-Plate for Phase-Contrast Transmission Electron Microscopy

2014 ◽  
Vol 20 (S3) ◽  
pp. 236-237
Author(s):  
Manuel Dries ◽  
Simon Hettler ◽  
Björn Gamm ◽  
Erich Müller ◽  
Winfried Send ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
Transmission Electron

A laser-based phase plate for phase contrast transmission electron microscopy

2018 ◽  
Author(s):  
S.L. Campbell ◽  
O. Schwartz ◽  
J. J. Axelrod ◽  
C. TUrnbaugh ◽  
R. M. Glaeser ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
Transmission Electron

New Electrostatic Phase Plate for Phase-Contrast Transmission Electron Microscopy and Its Application for Wave-Function Reconstruction

2010 ◽  
Vol 16 (6) ◽  
pp. 785-794 ◽  
Author(s):  
Katrin Schultheiss ◽  
Joachim Zach ◽  
Bjoern Gamm ◽  
Manuel Dries ◽  
Nicole Frindt ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Wave Function ◽  
Phase Contrast ◽  
Pt Nanoparticles ◽  
Ring Electrode ◽  
Phase Plate ◽  
Experimental Phase ◽  
Transmission Electron ◽  
Plate Design

AbstractA promising novel type of electrostatic phase plate for transmission electron microscopy (TEM) is presented. The phase plate consists of a single microcoaxial cable-like rod with its electrode exposed to the undiffracted electrons. The emerging field is used to shift the phase of the undiffracted electrons with respect to diffracted electrons. The design overcomes the drawback of the spatial frequency-blocking ring electrode of the Boersch phase plate. First, experimental phase-contrast images are presented for PbSe and Pt nanoparticles with clearly varying phase contrast, which depends on the applied voltage and resulting phase shift of the unscattered electrons. With the new phase-plate design, we show for the first time the reconstruction of an object wave function based on a series of only three experimental phase-contrast TEM images obtained with an electrostatic phase plate.

Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate

2007 ◽  
Vol 107 (2-3) ◽  
pp. 213-226 ◽  
Author(s):  
E. Majorovits ◽  
B. Barton ◽  
K. Schultheiß ◽  
F. Pérez-Willard ◽  
D. Gerthsen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
Transmission Electron

scholarly journals New developments in phase Contrast Transmission Electron Microscopy with Electrostatic Phase Plate

2009 ◽  
Vol 15 (S2) ◽  
pp. 1086-1087
Author(s):  
D Alloyeau ◽  
WK Hsieh ◽  
E Anderson ◽  
G Benner ◽  
MJ Park ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Contrast ◽  
Phase Plate ◽  
New Developments ◽  
Transmission Electron

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

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