scholarly journals Improving the Speed and Accuracy of Large-scale Scanning Transmission Electron Microscopy (STEM) Electron Scattering Simulations

2020 ◽  
Vol 26 (S2) ◽  
pp. 456-458
Author(s):  
Colin Ophus ◽  
Hamish Brown ◽  
Luis Rangel Dacosta ◽  
Philipp Pelz ◽  
Jonathan Schwartz ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Scattering ◽  
Scanning Transmission Electron Microscopy ◽  
Large Scale ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Speed And Accuracy

Scanning Transmission EM (STEM) of dynein ATPases

1987 ◽  
Vol 45 ◽  
pp. 798-799
Author(s):  
Kenneth A. Johnson ◽  
Silvio P. Marchese-Ragona ◽  
Joseph S. Wall
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Transmission Electron Microscopy ◽  
Electron Scattering ◽  
Scanning Transmission Electron Microscopy ◽  
Mass Analysis ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Lines Of Evidence

The structure and molecular weight of the microtubule-dependent ATPase, dynein, was first established by scanning transmission electron microscopy (STEM) of dynein isolated from the cilia of Tetrahymena. It was shown that dynein consists of three globular heads joined to a common base by three slender, flexible strands. The globular heads have a diameter of 10-12 nm and the strands are 24 nm in length, such that the particles are 35 nm overall. Mass analysis by integration of electron scattering intensities in the STEM established a molecular weight of 1.9 million, with each head contributing 420,000. Several lines of evidence suggested that the base anchors the dynein to the A-tubule and the three independent, globular heads interact with the B-tubule of the adjacent outerdoublet in an ATP-dependent reaction to produce a force for sliding.

Scanning Transmission Electron Microscopy of Polyethylene Crystals

1980 ◽  
Vol 38 ◽  
pp. 242-245
Author(s):  
F. Khoury ◽  
L. H. Bolz
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Crystallization Temperature ◽  
Scanning Transmission Electron Microscopy ◽  
Growth Habit ◽  
Lateral Growth ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Growth Habits

The lateral growth habits and non-planar conformations of polyethylene crystals grown from dilute solutions (<0.1% wt./vol.) are known to vary depending on the crystallization temperature.1-3 With the notable exception of a study by Keith2, most previous studies have been limited to crystals grown at <95°C. The trend in the change of the lateral growth habit of the crystals with increasing crystallization temperature (other factors remaining equal, i.e. polymer mol. wt. and concentration, solvent) is illustrated in Fig.l. The lateral growth faces in the lozenge shaped type of crystal (Fig.la) which is formed at lower temperatures are {110}. Crystals formed at higher temperatures exhibit 'truncated' profiles (Figs. lb,c) and are bound laterally by (110) and (200} growth faces. In addition, the shape of the latter crystals is all the more truncated (Fig.lc), and hence all the more elongated parallel to the b-axis, the higher the crystallization temperature.

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