Effect of column temperature on the direct determination of (RS)-.alpha.-cyano(3-phenoxyphenyl)methyl (RS)-2-(4-chlorophenyl)-3-methylbutyrate optical isomers by high-performance liquid chromatography/diode array system

1989 ◽  
Vol 61 (10) ◽  
pp. 1149-1151 ◽  
Author(s):  
Euphemia. Papadopoulou-Mourkidou
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Direct Determination ◽  
Diode Array ◽  
Optical Isomers ◽  
Column Temperature

Comparison Study on the Contents of Eight Flavonoids in three Different Processed Products of Scutellariae Radix using Ultra-high Performance Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

2020 ◽  
Vol 16 (6) ◽  
pp. 690-697
Author(s):  
Yuedong Yang ◽  
Hao Zhi ◽  
Baofei Yan ◽  
Yi Tian ◽  
Jianping Shen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Triple Quadrupole ◽  
Column Temperature ◽  
Scutellariae Radix ◽  
Total Percentage

Background: The simultaneous determination of multiple components in a sample is an important factor in the quality control of traditional Chinese medicines and can give an indication of potential clinical applications. Introduction: A rapid and sensitive method has been introduced for the simultaneous quantitative analysis of eight bioactive flavonoid constituents from Scutellariae Radix using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. Methods: The separation was performed on a Waters Acquity UPLC C18 column (2.1 mm×100 mm, 1.7 μm), under optimized mass spectrometry conditions, with a flow rate of 0.3 mL/min. The column temperature was maintained at 35°C and the injection volume was 3 μL. Results: The method showed a good linear relationship of each component; all R2 values were above 0.9990 in the experiment. The RSDs of the precision test, repeatability test, stability test and recovery test were all not more than 2.86 %. We found that the total percentage amounts of the eight flavonoids were 22.19%, 18.63% and 10.86% in Raw Scutellariae Radix (RSR), Wine Scutellaria Radix (WSR) and Scutellaria Radix Charcoal (SRC) respectively. Conclusion: The method was successfully applied to the simultaneous determination of the eight bioactive flavonoids of Raw Scutellariae Radix, Wine Scutellaria Radix and Scutellaria Radix Charcoal.

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