Evaluating Preparative Isoelectric Focusing of Complex Peptide Mixtures for Tandem Mass Spectrometry-Based Proteomics:  A Case Study in Profiling Chromatin-Enriched Subcellular Fractions inSaccharomycescerevisiae

2005 ◽  
Vol 77 (10) ◽  
pp. 3198-3207 ◽  
Author(s):  
Hongwei Xie ◽  
Sricharan Bandhakavi ◽  
Timothy J. Griffin
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Subcellular Fractions ◽  
Tandem Mass ◽  
Peptide Mixtures

scholarly journals Automated Capillary Isoelectric Focusing-Tandem Mass Spectrometry for Qualitative and Quantitative Top-Down Proteomics

2020 ◽  
Vol 92 (24) ◽  
pp. 15890-15898
Author(s):  
Tian Xu ◽  
Xiaojing Shen ◽  
Zhichang Yang ◽  
Daoyang Chen ◽  
Rachele A. Lubeckyj ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Tandem Mass ◽  
Capillary Isoelectric Focusing ◽  
Top Down ◽  
Qualitative And Quantitative

Microfluidic Platform for Liquid Chromatography−Tandem Mass Spectrometry Analyses of Complex Peptide Mixtures

2005 ◽  
Vol 77 (21) ◽  
pp. 6947-6953 ◽  
Author(s):  
Jun Xie ◽  
Yunan Miao ◽  
Jason Shih ◽  
Yu-Chong Tai ◽  
Terry D. Lee
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Microfluidic Platform ◽  
Chromatography Tandem Mass Spectrometry ◽  
Peptide Mixtures ◽  
Liquid Chromatography Tandem Mass

Newborn Screening for Sickle Cell Disease Using Tandem Mass Spectrometry

2008 ◽  
Vol 54 (12) ◽  
pp. 2036-2041 ◽  
Author(s):  
François Boemer ◽  
Olivier Ketelslegers ◽  
Jean-Marc Minon ◽  
Vincent Bours ◽  
Roland Schoos
Keyword(s):  
Mass Spectrometry ◽  
Sickle Cell Disease ◽  
Tandem Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Neonatal Screening ◽  
Limit Of Detection ◽  
Tandem Mass ◽  
Cell Disease ◽  
Hb E ◽  
Hb S

Abstract Background: Neonatal screening programs for sickle cell disease are now widespread in North American and European countries. Most programs apply isoelectric focusing or HPLC to detect hemoglobin variants. Because tandem mass spectrometry (MS/MS) is being used for screening of inherited metabolic disorders and allows protein identification, it was worth testing for hemoglobinopathy screening. Methods: We minimized sample preparation and analysis times by avoiding prior purification, derivatization, or separation. We developed a tryptic digestion methodology to screen for the main clinically important variants (Hb S, Hb C, and Hb E) and β-thalassemia. To ensure proper discrimination between homozygote and heterozygote variants, we selected 4 transitions with good signal intensities for each specific peptide and calculated variant/Hb A ratios for each. Method validation included intra- and interseries variability, carryover, and limit of detection. We also performed a comparative study with isoelectric focusing results on 2082 specimens. Results: Intraassay imprecision values (CVs) varied between 2.5% and 30.7%. Interassay CVs were between 6.3% and 23.6%. Carryover was <0.03%, and the limit of detection was fixed at 1% of Hb S. According to the MS/MS settings (detection of Hb S, Hb C, Hb E, and β-globin production defects), the comparative study did not yield any discrepant results between the 2 techniques. Conclusions: MS/MS is a reliable method for hemoglobinopathy neonatal screening.

scholarly journals Proteomic analysis of age dependent nitration of rat cardiac proteins by solution isoelectric focusing coupled to nanoHPLC tandem mass spectrometry

2007 ◽  
Vol 42 (7) ◽  
pp. 639-651 ◽  
Author(s):  
Sung Jung Hong ◽  
Giridharan Gokulrangan ◽  
Christian Schöneich
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Proteomic Analysis ◽  
Tandem Mass ◽  
Age Dependent

Added Value for Tandem Mass Spectrometry Shotgun Proteomics Data Validation through Isoelectric Focusing of Peptides

2005 ◽  
Vol 4 (6) ◽  
pp. 2273-2282 ◽  
Author(s):  
Manfred Heller ◽  
Mingliang Ye ◽  
Philippe E. Michel ◽  
Patrick Morier ◽  
Daniel Stalder ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Shotgun Proteomics ◽  
Added Value ◽  
Tandem Mass ◽  
Data Validation ◽  
Proteomics Data

scholarly journals Evaluation of Strong Cation Exchange versus Isoelectric Focusing of Peptides for Multidimensional Liquid Chromatography-Tandem Mass Spectrometry

2008 ◽  
Vol 7 (12) ◽  
pp. 5286-5294 ◽  
Author(s):  
Robbert J. C. Slebos ◽  
Jonathan W. C. Brock ◽  
Nancy F. Winters ◽  
Sarah R. Stuart ◽  
Misti A. Martinez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Cation Exchange ◽  
Isoelectric Focusing ◽  
Tandem Mass ◽  
Strong Cation Exchange ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass
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