Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle

2010 ◽  
Vol 82 (18) ◽  
pp. 7550-7555 ◽  
Author(s):  
Yancheng Guo ◽  
Babacar Ngom ◽  
Tao Le ◽  
Xiue Jin ◽  
Liping Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Detection ◽  
Immunochromatographic Assay ◽  
Chicken Muscle

scholarly journals Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fengli Liu ◽  
Yanxin Cao ◽  
Maokai Yan ◽  
Mengxu Sun ◽  
Qingshui Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Preventive Intervention ◽  
Virus Detection ◽  
Cross Reactivity ◽  
Duck Enteritis Virus ◽  
Detection Sensitivity ◽  
Immunochromatographic Assay ◽  
Efficient Detection ◽  
Enteritis Virus

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 103 TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.

scholarly journals Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip

2021 ◽  
Author(s):  
Zehua Xu ◽  
Tieqiang Sun ◽  
Hongwei He ◽  
Wentao Liu ◽  
Longxing Fan ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Immunochromatographic Assay

scholarly journals Development of a multicolor upconversion fluorescence immunoassay for the simultaneous detection of thiamethoxam and dextran by magnetic separation

RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 517-524
Author(s):  
Chuanyong Li ◽  
Wanlin Sun ◽  
Lianrun Huang ◽  
Nana Sun ◽  
Xiude Hua ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Determination ◽  
Magnetic Separation ◽  
Simultaneous Detection ◽  
Fluorescence Immunoassay ◽  
Upconversion Fluorescence

The anti-thiamethoxam and anti-dextran monoclonal antibodies were prepared to develop a multicolor upconversion fluorescence immunoassay for the simultaneous determination of thiamethoxam (544 nm) and dextran (477 nm).

scholarly journals Heterologous screening of hybridomas for the development of broad-specific monoclonal antibodies against deoxynivalenol and its analogues

2014 ◽  
Vol 7 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Y. Guo ◽  
M. Sanders ◽  
A. Galvita ◽  
A. Heyerick ◽  
D. Deforce ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Efficient Approach ◽  
Selection For ◽  
Similar Affinity

Hapten heterology was introduced into the steps of hybridoma selection for the development of monoclonal antibodies (MAbs) against deoxynivalenol (DON). Firstly, a novel heterologous DON hapten was synthesised and covalently coupled to proteins (i.e. bovine serum albumin (BSA), ovalbumin and horseradish peroxidase) using the linkage of cyanuric chloride (CC). After immunisation, antisera from different DON immunogens were checked for the presence of useful antibodies. Next, both homologous and heterologous enzyme-linked immunosorbent assays were conducted to screen for hybridomas. It was found that heterologous screening could significantly reduce the proportion of false positives and appeared to be an efficient approach for selecting hybridomas of interest. This strategy resulted in two kinds of broad-selective MAbs against DON and its analogues. They were quite distinct from other reported DON-antibodies in their cross-reactivity profiles. A unique MAb 13H1 derived from DON-CC-BSA immunogen could recognise DON and its analogues in the order of HT-2 toxin ≯ 15-acetyl-DON ≯ DON ≯ nivalenol, with IC50 ranging from 1.14 to 7.69 μg/ml. Another preferable MAb 10H10 generated from DON-BSA immunogen manifested relatively similar affinity to DON, 3-acetyl-DON and 15-acetyl-DON, with IC50 values of 22, 15 and 34 ng/ml, respectively. This is the first broad-specific MAb against DON and its two acetylated forms and thus it can be used for simultaneous detection of the three mycotoxins.

An Immunochromatographic Assay of 2,4-Dichlorophenoxyacetic Acid and Simazine Using Monoclonal Antibodies Labeled with Colloidal Gold

2004 ◽  
Vol 30 (2) ◽  
pp. 178-183 ◽  
Author(s):  
I. A. Lyubavina ◽  
A. A. Zinchenko ◽  
I. S. Salomatina ◽  
A. V. Zherdev ◽  
B. B. Dzantiev
Keyword(s):  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Immunochromatographic Assay ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid

Simultaneous detection of two independent antigens by double staining with two mouse monoclonal antibodies

1998 ◽  
Vol 73 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Norihiro Teramoto ◽  
Laszlo Szekely ◽  
Katja Pokrovskaja ◽  
Li Fu Hu ◽  
Tadashi Yoshino ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Detection ◽  
Double Staining

Clinical Evaluation of the CARDIAC STATus™, a Rapid Immunochromatographic Assay for Simultaneous Detection of Elevated Concentrations of CK-MB and Myoglobin in Whole Blood

1998 ◽  
Vol 36 (7) ◽  
Author(s):  
Yvonne Schouten ◽  
Robbert J. de Winter ◽  
Jozef P. M. C. Gorgels ◽  
Rudolph W. Koster ◽  
Rob Adams ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Whole Blood ◽  
Simultaneous Detection ◽  
Immunochromatographic Assay ◽  
Cardiac Status
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