Simultaneous detection of Entamoeba histolytica/dispar, Giardia duodenalis and cryptosporidia by immunochromatographic assay in stool samples from patients living in the Greater Cairo Region, Egypt

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

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Detection and Enumeration of the Commonest Stool Parasites Seen in a Tertiary Care Center in South India

ISRN Tropical Medicine ◽

10.1155/2013/808571 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6

Author(s):

Vani Chandrashekar

Keyword(s):

Entamoeba Histolytica ◽

South India ◽

Care Center ◽

Tertiary Care ◽

Age Group ◽

Tertiary Care Center ◽

Care Centre ◽

Tertiary Care Centre ◽

Stool Samples

The aim of this study was to identify common stool parasites in patients attending a tertiary care centre in South India. We evaluated 2355 stool samples and parasites were detected in 7.9% of samples. 41.1% of our patients were in the 45–58-year age group. Protozoal infections were the commonest seen in 7.8% of samples. Entamoeba histolytica was the commonest protozoa (4.6%) followed by Entamoeba coli (1.2%) and Giardia (0.8%). Entamoeba histolytica and Entamoeba coli were together seen in 0.63%, and they were the commonest organisms seen in samples with multiple-organism infection. Both were equally detected in diarrheal samples.

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Simultaneous Detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in Fecal Samples by Using Multiplex Real-Time PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.42.3.1220-1223.2004 ◽

2004 ◽

Vol 42 (3) ◽

pp. 1220-1223 ◽

Cited By ~ 255

Author(s):

J. J. Verweij ◽

R. A. Blange ◽

K. Templeton ◽

J. Schinkel ◽

E. A. T. Brienen ◽

...

Keyword(s):

Real Time ◽

Entamoeba Histolytica ◽

Cryptosporidium Parvum ◽

Real Time Pcr ◽

Giardia Lamblia ◽

Simultaneous Detection ◽

Fecal Samples

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Prevalence of Entamoeba histolytica and Giardia lamblia in Children in Kadhmiyah Hospital

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v36i1.543 ◽

2012 ◽

Vol 36 (1) ◽

pp. 1-5

Author(s):

Amjed Qays Ibrahim

Keyword(s):

Entamoeba Histolytica ◽

Giardia Lamblia ◽

Age Group ◽

Significant Relation ◽

Female Ratio ◽

Male Ratio ◽

Infectivity Rate ◽

Stool Samples

In this study we collect 1520 stool samples during the period from September to December 2010 from children whom their ages between 1 month - 12 years. The results showed that the total infection of Entamoeba histolytica was 9.80% , and Giardia lamblia was 1.77%. And the male ratio that infected with Entamoeba histolytica was 9.83% , while the female ratio was 9.74%; and the male infected with Giardia lamblia was 1.51% , while the female ratio was 2.18%. The result showed that the high average of infection with Entamoeba histolytica and Giardia lamblia in age group from 1 month to 2 years. And there is no significance difference between gender and infectivity rate of Entamoeba histolytica and Giardia lamblia under P≤0.05. Also it showed that there were significant relation between Age group and infectivity rate of Entamoeba histolytica and Giardia lamblia.

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