Dissecting the Large Noncovalent Protein Complex GroEL with Surface-Induced Dissociation and Ion Mobility–Mass Spectrometry

2013 ◽  
Vol 85 (17) ◽  
pp. 8262-8267 ◽  
Author(s):  
Mowei Zhou ◽  
Christopher M. Jones ◽  
Vicki H. Wysocki
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Complex ◽  
Ion Mobility Mass Spectrometry ◽  
Surface Induced Dissociation ◽  
Noncovalent Protein Complex ◽  
Noncovalent Protein

Possible isomers in ligand protected Ag11cluster ions identified by ion mobility mass spectrometry and fragmented by surface induced dissociation

2016 ◽  
Vol 52 (19) ◽  
pp. 3805-3808 ◽  
Author(s):  
Ananya Baksi ◽  
Sophie R. Harvey ◽  
Ganapati Natarajan ◽  
Vicki H. Wysocki ◽  
Thalappil Pradeep
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Collision Induced Dissociation ◽  
Silver Clusters ◽  
Ion Mobility Mass Spectrometry ◽  
Surface Induced Dissociation

Isomeric glutathione protected silver clusters have been detected using ion mobility mass spectrometry. This cluster has been fragmented by conventional collision induced dissociation and newly introduced surface induced dissociation.

Illustration of SID-IM-SID (surface-induced dissociation-ion mobility-SID) mass spectrometry: homo and hetero model protein complexes

The Analyst ◽  
2015 ◽  
Vol 140 (20) ◽  
pp. 7012-7019 ◽  
Author(s):  
Royston S. Quintyn ◽  
Sophie R. Harvey ◽  
Vicki H. Wysocki
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Protein Complex ◽  
Protein Complexes ◽  
Second Stage ◽  
Surface Induced Dissociation ◽  
Model Protein ◽  
Complex Architecture

Surface collisions generate subcomplexes, which are then separated by ion mobility and dissociated into their individual subunitsviaa second stage of surface collisions to elucidate protein complex architecture and assembly.

scholarly journals Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell

The Analyst ◽  
2016 ◽  
Vol 141 (3) ◽  
pp. 884-891 ◽  
Author(s):  
Samuel J. Allen ◽  
Kevin Giles ◽  
Tony Gilbert ◽  
Matthew F. Bush
Keyword(s):  
Mass Spectrometry ◽  
Radio Frequency ◽  
Cross Sections ◽  
Ion Mobility ◽  
Protein Complex ◽  
Biological Molecules ◽  
Ion Mobility Mass Spectrometry ◽  
Complex Ions ◽  
Drift Cell ◽  
Peak Broadening

A new drift cell was used to measure collision cross sections and characterize the origins of ion mobility peak broadening for biological molecules and assemblies.

scholarly journals Surface induced dissociation as a tool to study membrane protein complexes

2017 ◽  
Vol 53 (21) ◽  
pp. 3106-3109 ◽  
Author(s):  
Sophie R. Harvey ◽  
Yang Liu ◽  
Wen Liu ◽  
Vicki H. Wysocki ◽  
Arthur Laganowsky
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Ion Mobility ◽  
Protein Complexes ◽  
Integral Membrane Protein ◽  
Ion Mobility Mass Spectrometry ◽  
Surface Induced Dissociation ◽  
Membrane Protein Complexes

Native ion mobility mass spectrometry (MS) and surface induced dissociation (SID) are applied to study the integral membrane protein complexes AmtB and AqpZ.

Sign in / Sign up

Export Citation Format

Share Document