Label-Free In-Flow Detection of Single DNA Molecules using Glass Nanopipettes

2013 ◽  
Vol 86 (1) ◽  
pp. 835-841 ◽  
Author(s):  
Xiuqing Gong ◽  
Amol V. Patil ◽  
Aleksandar P. Ivanov ◽  
Qingyuan Kong ◽  
Thomas Gibb ◽  
...  
Keyword(s):  
Label Free ◽  
Dna Molecules ◽  
Single Dna Molecules ◽  
Flow Detection

scholarly journals Active Delivery of Single DNA Molecules into a Plasmonic Nanopore for Label-Free Optical Sensing

Nano Letters ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 8003-8010 ◽  
Author(s):  
Xin Shi ◽  
Daniel V. Verschueren ◽  
Cees Dekker
Keyword(s):  
Optical Sensing ◽  
Label Free ◽  
Dna Molecules ◽  
Single Dna Molecules

Thermoplastic Nanofluidic Devices for Identifying Abasic Sites in Single DNA Molecules

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Steven A Soper ◽  
Swarnagowri Vaidyanathan ◽  
Franklin Uba ◽  
Bo Hu ◽  
David Kaufman ◽  
...  
Keyword(s):  
Dna Damage ◽  
Therapeutic Efficacy ◽  
Double Strand Breaks ◽  
Dna Molecules ◽  
Strand Breaks ◽  
Single Dna Molecules ◽  
Abasic Sites ◽  
Nanofluidic Devices ◽  
Ap Sites

DNA damage can take many forms such as double-strand breaks and/or the formation of abasic (apurinic/apyrimidinic; AP) sites. The presence of AP sites can be used to determine therapeutic efficacy...

ChemInform Abstract: Photobleaching of Asymmetric Cyanines Used for Fluorescence Imaging of Single DNA Molecules.

ChemInform ◽  
2010 ◽  
Vol 32 (49) ◽  
pp. no-no
Author(s):  
Claire Kanony ◽  
Bjoern Aakerman ◽  
Eimer Tuite
Keyword(s):  
Fluorescence Imaging ◽  
Dna Molecules ◽  
Single Dna Molecules

Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads

Science ◽  
1992 ◽  
Vol 258 (5085) ◽  
pp. 1122-1126 ◽  
Author(s):  
S. Smith ◽  
L Finzi ◽  
C Bustamante
Keyword(s):  
Magnetic Beads ◽  
Dna Molecules ◽  
Single Dna Molecules ◽  
Mechanical Measurements

TOWARDS SENSING SINGLE OR A FEW BIO-MOLECULAR ARCHITECTURES: DESIGN OF FUNCTIONAL SURFACES

2008 ◽  
Vol 18 (01) ◽  
pp. 187-194
Author(s):  
PEIJI ZHAO ◽  
DWIGHT WOOLARD ◽  
JORGE M. SEMINARIO ◽  
ROBERT TREW
Keyword(s):  
Density Functional ◽  
High Sensitivity ◽  
Lower Sensitivity ◽  
Label Free ◽  
Dna Molecules ◽  
Surface Attachment ◽  
Biomolecular Sensing ◽  
Aromatic Molecules ◽  
Electrical Sensing ◽  
Silicon Based

There is considerable interest in electrical sensing of biomolecular binding since it has the potential to be label free, to work easily in aqueous environments native to the biomolecules, and to be integrated with small, fast, and inexpensive microelectronoics as detection instrumentation. Although electrochemical methods have been used successfully in detections of DNA molecules with Ag labels at very high sensitivity (~ p ml), detection of DNA molecules in terms of label free techniques has a lower sensitivity (~ μ ml). Here, the surface attachment chemistry is critical towards the detection of ultra-low concentration of biomolecules. In this article, based on density functional theory, we have calculated and analyzed the electrical characteristics of the contact between aromatic molecules and silicon (100) − 2×1 surfaces. Design principles for silicon based electrodes of electrochemically biomolecular sensing instruments for label-free sensing of single or a few biomolecular molecules have also been discussed.

scholarly journals Molecular conformations of DNA targets captured by model nanoarrays

Nanoscale ◽  
2017 ◽  
Vol 9 (36) ◽  
pp. 13419-13424 ◽  
Author(s):  
X. Hao ◽  
E. A. Josephs ◽  
Q. Gu ◽  
T. Ye
Keyword(s):  
Dna Molecules ◽  
Molecular Conformations ◽  
Single Dna Molecules

We generated nanoarrays with tailored surface functionalities and morphologies to probe how single DNA molecules interact with surface heterogeneities.

scholarly journals Somatic mutation landscapes at single-molecule resolution

2021 ◽  
Author(s):  
Stefanie V. Lensing ◽  
Peter Ellis ◽  
Federico Abascal ◽  
Iñigo Martincorena ◽  
Robert J. Osborne
Keyword(s):  
Single Molecule ◽  
Single Cells ◽  
Error Rates ◽  
Dna Molecules ◽  
Base Pairs ◽  
Single Dna Molecules ◽  
Sequencing Library ◽  
Somatic Mutagenesis ◽  
Qpcr Quantification ◽  
Duplex Sequencing

Abstract Somatic mutations drive cancer development and may contribute to ageing and other diseases. Yet, the difficulty of detecting mutations present only in single cells or small clones has limited our knowledge of somatic mutagenesis to a minority of tissues. To overcome these limitations, we introduce nanorate sequencing (NanoSeq), a new duplex sequencing protocol with error rates <5 errors per billion base pairs in single DNA molecules from cell populations. The version of the protocol described here uses clean genome fragmentation with a restriction enzyme to prevent end-repair-associated errors and ddBTPs/dATPs during A-tailing to prevent nick extension. Both changes reduce the error rate of standard duplex sequencing protocols by preventing the fixation of DNA damage into both strands of DNA molecules during library preparation. We also use qPCR quantification of the library prior to amplification to optimise the complexity of the sequencing library given the desired sequencing coverage, maximising duplex coverage. The sample preparation protocol takes between 1 and 2 days, depending on the number of samples processed. The bioinformatic protocol is described in:https://github.com/cancerit/NanoSeqhttps://github.com/fa8sanger/NanoSeq_Paper_Code

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