Optimal Staining Conditions for the Quantitative Analysis of Human Serum Protein Fractions by Cellulose Acetate Electrophoresis

1960 ◽  
Vol 32 (10) ◽  
pp. 1353-1356 ◽  
Author(s):  
Colin J. Brackenridge
Keyword(s):  
Quantitative Analysis ◽  
Human Serum ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Fractions ◽  
Cellulose Acetate Electrophoresis ◽  
Human Serum Protein

Imprecision of quantification of serum protein fractions by electrophoresis on cellulose acetate.

1986 ◽  
Vol 32 (2) ◽  
pp. 356-357 ◽  
Author(s):  
S N Kahn ◽  
L P Strony
Keyword(s):  
High Resolution ◽  
Serum Albumin ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Fractions ◽  
Visual Interpretation ◽  
Cellulose Acetate Electrophoresis ◽  
Trained Observer ◽  
Electrophoretic Patterns

Abstract We studied the precision of densitometric quantification of the protein zones resolved by cellulose acetate electrophoresis. Replicate analyses of patients' samples by a single technologist showed mean CVs ranging from 2.9% for serum albumin to 9.5% for alpha 1-globulin. There were marked differences in measurements obtained by replicate analysis of the same samples by two experienced technologists. We calculated what changes in fractional concentrations would be analytically significant and concluded that densitometry of cellulose acetate electrophoretograms can only be semi-quantitative. We suggest that visual interpretation of high-resolution electrophoretic patterns by a trained observer can replace densitometry in most cases.

The interaction of human serum protein fractions with the starch-iodine complex

1965 ◽  
Vol 12 (6) ◽  
pp. 631-638 ◽  
Author(s):  
R.L. Searcy ◽  
S. Hayashi ◽  
E.M. Hardy ◽  
J.E. Berk
Keyword(s):  
Human Serum ◽  
Serum Protein ◽  
Protein Fractions ◽  
Iodine Complex ◽  
Human Serum Protein

Rapid Cellulose Acetate Electrophoresis

1963 ◽  
Vol 9 (3) ◽  
pp. 317-324 ◽  
Author(s):  
Raymond C Bartlett
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Linear Response ◽  
Protein Fractions ◽  
Cellulose Acetate Electrophoresis ◽  
Binding Characteristics ◽  
Amido Black ◽  
Dye Binding

Abstract A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and γ-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has afforded excellent quantitations of serum protein fractions.

scholarly journals On the standard procedure for the analysis of serum protein fractions by cellulose acetate electrophoresis

1966 ◽  
Vol 11 (4) ◽  
pp. 351-356
Author(s):  
Y. Ogawa ◽  
M. Abe ◽  
M. Kitamura ◽  
N. Kosakai ◽  
K. Shimao ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Standard Procedure ◽  
Protein Fractions ◽  
Cellulose Acetate Electrophoresis
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