Determination of Optimum Solvent Systems for Countercurrent Distribution from Paper Chromatographic Data. Binary Organic Solvent/Formamide Systems.

Paper chromatographic data are reported on 220 aromatic compounds of biological interest, including phenolic acids, indoles, imidazoles, purines, pyrimidines, and some miscellaneous substances. Rf data in four solvent systems and color reactions with 12 spray reagents are tabulated. Some correlations between structure of these compounds, relative Rf, and color with various spray reagents are noted.

Download Full-text

Determination of the Azeotropic Composition for Cyclohexane and Ethanol Using Gas Chromatographic Data

Journal of Chemical Education ◽

10.1021/ed086p764.1 ◽

2009 ◽

Vol 86 (6) ◽

pp. 764

Author(s):

Beatriz H. Cardelino

Keyword(s):

Chromatographic Data

Download Full-text

Newly Developed Highly Sensitive Method for the Determination of Capecitabine by Using UV-Spectroscopy

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2019.110304 ◽

2019 ◽

Vol 11 (03) ◽

Author(s):

Manas Ranjan Mishra ◽

Punam Agrawal ◽

Surya Narayan Das

Keyword(s):

Colon Cancer ◽

Uv Spectroscopy ◽

First Line ◽

Spectrophotometric Methods ◽

Advanced Colon Cancer ◽

Ich Guidelines ◽

Highly Sensitive ◽

Solvent Systems ◽

Sensitive Method

Capecitabine is a 'pro-drug' to the cytotoxic agent 5-fluorouracil (5-FU) intended to administered orally. Capecitabine is generally used as first line monotherapy for advanced colon cancer. Simple, rapid, accurate UV spectrophotometric methods were developed in the present study and validated for the estimation of Capecitabine in bulk and its formulations as per ICH guidelines. Three solvent systems viz., 0.1N NaOH, 0.1N HCl and Methanol: Water (1:3) was tried. The results suggest that the developed method shows linearity over the range of concentration 2-24μg/ml and a correlation coefficient of 0.9999. Accuracy, precision, linearity, robustness, and ruggedness were statistically validated as per ICH guidelines for all the developed methods. The % RSD values for validated methods were found to be less than 1.5 and methods will find application in routine analysis of drug formulations containing Capecitabine.

Download Full-text

With Solvent Extraction Method, and via new Organic Reagent 2-(Benzo thiazolyl azo)-4,5- Diphenyl Imidazole for Spectrophotometric Determination of Copper (II) in different Samples

Baghdad Science Journal ◽

10.21123/bsj.11.1.147-157 ◽

2014 ◽

Vol 11 (1) ◽

pp. 147-157

Author(s):

Baghdad Science Journal

Keyword(s):

Organic Solvent ◽

Spectrophotometric Determination ◽

Extraction Method ◽

Complex Structure ◽

Organic Reagent ◽

Complexing Agent ◽

Solvent Extraction Method ◽

Determination Of Copper ◽

Synergism Effect

The new organic reagent 2-[Benzo thiazolyl azo]-4,5-diphenyl imidazole was prepared and used as complexing agent for separation and spectrophotometric determination of Cu2+ ion in some samples include plants, soil, water and human blood serum. Initially determined all factors effect on extraction method and the results show optimum pH was (pHex=9), optimum concentration was 40?g/5mLCu2+ and optimum shaking time was (15min.), as well stoichiometry study appears the complex structure was 1:1 Cu2+: BTADPI. Interferences effect of cations were studied. Synergism effect shows MIBK gave increasing in distribution ratio (D). Organic solvent effect appears there is no any linear relation between dielectric constant for organic solvent used and distribution ration (D). Thermodynamically found the reaction was Endothermic reaction, with ?Hex= 0.0131 KJ.mole-1,?Gex=-54.20 KJ.mole-1 ,?Sex= 167.84 J.mole-1.Beer’s law was obeyed over the concentration 1-30?g/5mL, and ?=922.90 Lmol-1.cm-1,with detection limit 1.7×10-5and Sandell’s sensitivity 6.8× 10-7 gcm-2.

Download Full-text